{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,18]],"date-time":"2026-03-18T13:40:22Z","timestamp":1773841222128,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T00:00:00Z","timestamp":1715126400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100005294","name":"Massachusetts General Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100005294","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Oral Rehabilitation"],"published-print":{"date-parts":[[2024,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Current clinical approaches to regenerate temporomandibular joint (TMJ) articulating cartilage defects only treat the symptoms (i.e. pain and dysfunction) and do not seek to restore joint integrity for long\u2010term relief. Therefore, we investigated a novel self\u2010assembling tissue\u2010engineered cartilage to overcome this significant clinical issue for TMJ regenerative purposes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Objectives<\/jats:title><jats:p>Examine the maturation of dynamic self\u2010regenerating cartilage (dSRC) using auricular chondrocytes and evaluate a novel combinatorial approach with fractional laser treatment and dSRC implantation for TMJ cartilage repair.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>A suspension of 10<jats:sup>7<\/jats:sup> freshly harvested rabbit ear chondrocytes was cultured under a continuous reciprocating motion to form the dSRC. After 2, 4 and 8\u2009weeks of culture, dSRC samples were stained with H&amp;E, Safranin\u2010O and Toluidine Blue. Immunohistochemistry (IHC) was performed for collagens type I and II. Channels (300\u2013500\u2009\u03bcm diameter and 1.2\u20131.5\u2009mm depth) were created in six freshly harvested condyles using a fractional Erbium laser. Two groups were tested: dSRC in a laser\u2010ablated lesion (experimental) and an empty laser\u2010ablated channel (control). TMJ condyles were cultured for up to 8\u2009weeks and analysed as described above.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>H&amp;E staining showed a high cell density in dSRC compared to native cartilage. All dSRC groups demonstrated intense Safranin\u2010O staining, indicating high glycosaminoglycan (GAG) production and intense Toluidine Blue staining showed high proteoglycan content. IHC confirmed that dSRC consisted predominantly of collagen type II. The experimental group showed improved cartilage repair at both time points compared to the empty channels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>dSRC viability and successful matrix formation were demonstrated in\u00a0vitro. The combination of fractional laser ablation and dSRC implantation enhanced cartilage repair.<\/jats:p><\/jats:sec>","DOI":"10.1111\/joor.13728","type":"journal-article","created":{"date-parts":[[2024,5,8]],"date-time":"2024-05-08T07:44:49Z","timestamp":1715154289000},"page":"1521-1529","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["A regenerative approach for temporomandibular joint repair: An in\u00a0vitro and ex\u00a0vivo study"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8554-8849","authenticated-orcid":false,"given":"Fernando P. S.","family":"Guastaldi","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Massachusetts General Hospital, Harvard School of Dental Medicine  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrique R.","family":"Matheus","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Massachusetts General Hospital, Harvard School of Dental Medicine  Boston Massachusetts USA"},{"name":"Department of Diagnosis and Surgery, Division of Periodontics S\u00e3o Paulo State University (UNESP), School of Dentistry  Ara\u00e7atuba SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrique","family":"Hadad","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Massachusetts General Hospital, Harvard School of Dental Medicine  Boston Massachusetts USA"},{"name":"Department of Diagnosis and Surgery, Oral &amp; Maxillofacial Surgery Division S\u00e3o Paulo State University (UNESP), School of Dentistry  Ara\u00e7atuba SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark A.","family":"Randolph","sequence":"additional","affiliation":[{"name":"Division of Plastic and Reconstructive Surgery Massachusetts General Hospital, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert W.","family":"Redmond","sequence":"additional","affiliation":[{"name":"Wellman Center for Photomedicine Massachusetts General Hospital, Harvard Medical School  Boston Massachusetts USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2024,5,8]]},"reference":[{"key":"e_1_2_10_2_1","volume-title":"StatPearls","author":"Bordoni B","year":"2021"},{"key":"e_1_2_10_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jgs.15354"},{"key":"e_1_2_10_4_1","unstructured":"National Institute of Dental and Craniofacial Research. 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