{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T07:16:03Z","timestamp":1778656563648,"version":"3.51.4"},"reference-count":28,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"},{"start":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T00:00:00Z","timestamp":1771977600000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J of Oral Rehabilitation"],"published-print":{"date-parts":[[2026,6]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Unstimulated whole saliva (UWS) sialometry is fundamental for diagnosing salivary gland hypofunction, but single\u2010timepoint measurements are limited by physiological variability.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To evaluate intraindividual variability and reproducibility of a repeated\u2010measures UWS collection protocol in healthy young adults, and to propose a standardised approach to improve diagnostic accuracy.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Sixty\u2010two healthy subjects (19\u201333\u2009years old) collected UWS by passive drooling three times daily (morning, afternoon and evening) over three consecutive days (nine timepoints). Data were analysed using linear mixed\u2010effects models, intraclass correlation coefficients (ICC), Bland\u2013Altman plots and coefficients of variation (CV%).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Mean UWS flow rate collected over 5\u2009min remained stable across days (0.38\u20130.39\u2009mL\/min), ranging from 0.14 to 0.93\u2009mL\/min. Aggregated daily means showed the highest reproducibility (ICC\n                      <jats:sub>3<\/jats:sub>\n                      \u2009=\u20090.890 for Days 1\u20132), while single timepoints were less consistent (afternoon ICC\n                      <jats:sub>3<\/jats:sub>\n                      \u2009=\u20090.524). Evening collections demonstrated the best reproducibility among individual timepoints (ICC\n                      <jats:sub>3<\/jats:sub>\n                      \u2009=\u20090.751). Bland\u2013Altman analysis indicated low bias and acceptable agreement across days; within\u2010subject variability (CVw%) was 32.24%.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Single UWS measurements are influenced by substantial physiological variability, which may lead to diagnostic misclassification. Averaging multiple standardised collections, particularly in the evening, enhances reliability and may optimise diagnostic accuracy. This protocol supports refinement of clinical guidelines and the implementation of home\u2010based saliva testing for salivary gland dysfunction.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/joor.70172","type":"journal-article","created":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T12:35:55Z","timestamp":1772973355000},"page":"1169-1176","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Repeated Unstimulated Whole Saliva Collection: A Reliable Approach to Improve Diagnostic Accuracy"],"prefix":"10.1111","volume":"53","author":[{"ORCID":"https:\/\/orcid.org\/0009-0005-3576-4670","authenticated-orcid":false,"given":"Gennaro","family":"Musella","sequence":"first","affiliation":[{"name":"Department of Clinical and Experimental Medicine University of Foggia  Foggia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Filippo","family":"Sisto","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine University of Foggia  Foggia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Eleonora","family":"Bizzoca","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine University of Foggia  Foggia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicola","family":"Cirillo","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine University of Foggia  Foggia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lorenzo","family":"Lo Muzio","sequence":"additional","affiliation":[{"name":"Department of Clinical and Experimental Medicine University of Foggia  Foggia Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5647-4414","authenticated-orcid":false,"given":"Giuseppe","family":"Troiano","sequence":"additional","affiliation":[{"name":"Department of Medicine and Surgery University LUM  Casamassima Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosa Mar\u00eda","family":"L\u00f3pez\u2010Pintor","sequence":"additional","affiliation":[{"name":"ORALMED Research Group, Department of Dental Clinical Specialties, School of Dentistry Complutense University  Madrid Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5080-5921","authenticated-orcid":false,"given":"Vito Carlo Alberto","family":"Caponio","sequence":"additional","affiliation":[{"name":"ORALMED Research Group, Department of Dental Clinical Specialties, School of Dentistry Complutense University  Madrid Spain"},{"name":"Department of Life Sciences, Health and Health Professions Link Campus University  Rome Italy"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2026,2,25]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.1067\/mpr.2001.113778"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1186\/s42358-020-00158-0"},{"key":"e_1_2_11_4_1","first-page":"45","article-title":"Diagnosis and Management of Xerostomia and Hyposalivation","volume":"11","author":"Villa A.","year":"2015","journal-title":"Therapeutics and Clinical Risk Management"},{"issue":"4","key":"e_1_2_11_5_1","first-page":"219","article-title":"The Unstimulated Salivary Flow Rate in a Jordanian Healthy Adult Population","volume":"1","author":"Sawair F. 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