{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,17]],"date-time":"2026-04-17T01:29:52Z","timestamp":1776389392984,"version":"3.51.2"},"reference-count":31,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,11,27]],"date-time":"2012-11-27T00:00:00Z","timestamp":1353974400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Pemphigus vulgaris (PV) is an autoimmune mucocutaneous disease presenting clinically with blisters or erosions of the skin and mucous membrane. The main histopathologic characteristic of this disease is suprabasal vesicles due to loss of cell\u2013cell adhesion between keratinocytes named acantholysis. Studies have shown that apoptosis is increased in PV. The purpose of this study is to investigate the role of apoptosis in blister formation in PV.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This cross\u2010sectional study was conducted on 25 specimens of oral PV. The presence of apoptosis was evaluated using the <jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content> technique in the normal perilesional region, vesicle area, and acantholytic cells. Also, the expression of Bax pro\u2010apoptotic marker was assessed by the biotin\u2013streptavidin immunohistochemical method. <jats:styled-content style=\"fixed-case\">SPSS<\/jats:styled-content> software was used for Wilcoxon test analysis. <jats:italic>P<\/jats:italic> values &lt;0.05 were considered significant.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The percentage and intensity staining of <jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content>\u2010positive cells were noteworthy. There were statistically significant differences between basal and parabasal (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.05), tombstone with vesicle roof (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.038) and basal with tombstone (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.038). However, the expression and staining intensity of pro\u2010apoptotic marker Bax were weak, and no statistically significant differences were observed between the various areas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results obtained in the present study suggest that the process of apoptosis occurs early in PV because it was observed in the perilesional normal appearing tissue. Also, the process of apoptosis may cause exacerbation or speeding of the bulla formation. In other words, inhibition of apoptosis in the patients could reduce the severity of the lesions. .<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12022","type":"journal-article","created":{"date-parts":[[2012,11,27]],"date-time":"2012-11-27T22:47:46Z","timestamp":1354056466000},"page":"409-414","source":"Crossref","is-referenced-by-count":16,"title":["Study of apoptosis in oral pemphigus vulgaris using immunohistochemical marker Bax and TUNEL technique"],"prefix":"10.1111","volume":"42","author":[{"given":"Parviz","family":"Deyhimi","sequence":"first","affiliation":[{"name":"Torabinegad Dental Research Center and Department of Oral &amp; Maxillofacial Pathology School of Dentistry, Isfahan University of Medical Sciences Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Payam","family":"Tavakoli","sequence":"additional","affiliation":[{"name":"Torabinegad Dental Research Center and Department of Oral &amp; Maxillofacial Pathology School of Dentistry, Isfahan University of Medical Sciences Isfahan Iran"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,11,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjoms.2007.07.205"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00418-008-0420-0"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s004030050347"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1023\/B:APPT.0000018795.05766.1f"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)61239-4"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1313.040"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1080\/08916930600971836"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.03.107"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2003.12014.x"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-313X.2004.02040.x"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.genet.33.1.29"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4401186"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.17.1.331"},{"key":"e_1_2_6_15_1","first-page":"219","article-title":"Differentiation between cellular apoptosis and necrosis by the combined use of in situ tailing and nick translation techniques","volume":"71","author":"Gold R","year":"1994","journal-title":"Lab Invest"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1080\/000155598441800"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2004.05919.x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00161.2008"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.3109\/08916934.2011.606444"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0625.2009.00934.x"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2230.2011.04092.x"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2009.01.011"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12274679"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200605125"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M611365200"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2009.03162.x"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.5858\/133.1.97"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.182.1.711"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2133.2006.07654.x"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1747.2000.00835.x"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-4362.2001.01083.x"},{"key":"e_1_2_6_32_1","first-page":"200","article-title":"Soluble Fas in pemphigus vulgaris","volume":"14","author":"Moravvej H","year":"2011","journal-title":"Arch Iran Med"}],"container-title":["Journal of Oral Pathology &amp; 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