{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T07:33:51Z","timestamp":1776497631046,"version":"3.51.2"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2012,12,10]],"date-time":"2012-12-10T00:00:00Z","timestamp":1355097600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology","award":["22390388"],"award-info":[{"award-number":["22390388"]}]},{"name":"Scientific Research from the Ministry of Education, Culture, Sports, Science and Technology","award":[". 23792365"],"award-info":[{"award-number":[". 23792365"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>During tumor development, cells are exposed to a hypoxic microenvironment. Tumor hypoxia also has a profound influence on the sensitivity of cancer chemotherapy.<\/jats:p><jats:p>The objective of this study was to investigate the mechanism of cisplatin (<jats:styled-content style=\"fixed-case\">CDDP<\/jats:styled-content>) resistance of oral squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content>) cells under hypoxia by analyzing gene expression profiles to identify key genes and factors involved.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Cell viability was measured following culture of the cells in the presence or absence of CDDP, under normoxic or hypoxic conditions, using a CCK\u20108 assay. Analysis of the expression of HIF target genes in hypoxia\u2010treated cells was performed using an HIF\u2010regulated <jats:styled-content style=\"fixed-case\">cDNA<\/jats:styled-content> plate array. Changes in the <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression of selected HIF target genes were analyzed using RT\u2010PCR, and changes in the protein levels of these genes were analyzed by Western blotting. Tumor cell apoptosis was assessed by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content> cell lines responded differently to <jats:styled-content style=\"fixed-case\">CDDP<\/jats:styled-content> under normoxic and hypoxic conditions. The expression of glucose transporter protein\u20101 (<jats:styled-content style=\"fixed-case\">GLUT<\/jats:styled-content>\u20101) was up\u2010regulated in human squamous cell carcinoma of mouth (HSC\u20102) cells under hypoxia. Furthermore, there was little correlation between the cisplatin sensitivity of <jats:styled-content style=\"fixed-case\">human squamous cell carcinoma of tongue (SAS)<\/jats:styled-content> in normoxia and hypoxia. After <jats:styled-content style=\"fixed-case\">GLUT<\/jats:styled-content>\u20101 knockdown, <jats:styled-content style=\"fixed-case\">CDDP<\/jats:styled-content> treatment resulted in increased rates of apoptosis under hypoxia as compared with normoxia in cell lines <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>\u20102, Ca9\u201022, and <jats:styled-content style=\"fixed-case\">SAS<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>=\u00a00.025).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results of this study suggest that knockdown of <jats:styled-content style=\"fixed-case\">GLUT<\/jats:styled-content>\u20101 inhibits sensitization of oral squamous cells to <jats:styled-content style=\"fixed-case\">CDDP<\/jats:styled-content> during hypoxia in <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>\u20102, Ca9\u201022, and <jats:styled-content style=\"fixed-case\">SAS<\/jats:styled-content> cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12028","type":"journal-article","created":{"date-parts":[[2012,12,12]],"date-time":"2012-12-12T03:08:01Z","timestamp":1355281681000},"page":"382-388","source":"Crossref","is-referenced-by-count":40,"title":["Silencing of <scp>GLUT<\/scp>\u20101 inhibits sensitization of oral cancer cells to cisplatin during hypoxia"],"prefix":"10.1111","volume":"42","author":[{"given":"Makoto","family":"Shimanishi","sequence":"first","affiliation":[{"name":"Department of Oral Surgery Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Ogi","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yohei","family":"Sogabe","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Takeshi","family":"Kaneko","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hironari","family":"Dehari","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Akihiro","family":"Miyazaki","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery Sapporo Medical University School of Medicine Sapporo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiroyoshi","family":"Hiratsuka","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery Sapporo Medical University School of Medicine Sapporo 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