{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T20:26:35Z","timestamp":1775766395196,"version":"3.50.1"},"reference-count":46,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,1,18]],"date-time":"2013-01-18T00:00:00Z","timestamp":1358467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2013,7]]},"abstract":"Background<\/jats:title>Interleukin\u20101\u03b2 (IL<\/jats:styled-content>\u20101\u03b2) and tumor necrosis factor\u2010\u03b1 (TNF<\/jats:styled-content>\u2010\u03b1) are key mediators of the intracapsular pathological conditions of the temporomandibular joint (TMJ<\/jats:styled-content>). Therefore, the gene expression profiles in synovial fibroblast\u2010like cells (SFC<\/jats:styled-content>s) from patients with internal derangement of the TMJ<\/jats:styled-content> were examined after they were stimulated with IL<\/jats:styled-content>\u20101\u03b2 or TNF<\/jats:styled-content>\u2010\u03b1 to determine which genes were altered.<\/jats:p><\/jats:sec>Methods<\/jats:title>Ribonucleic acid was isolated from SFC<\/jats:styled-content>s after IL<\/jats:styled-content>\u20101\u03b2 or TNF<\/jats:styled-content>\u2010\u03b1 treatment. Gene expression profiling was performed using oligonucleotide microarray analysis. On the basis of the results of this assay, we investigated the kinetics of macrophage inflammatory protein\u20103\u03b1 (MIP<\/jats:styled-content>\u20103\u03b1) gene expression using PCR<\/jats:styled-content>, and protein production in TMJ SFC<\/jats:styled-content>s stimulated by IL<\/jats:styled-content>\u20101\u03b2 or TNF<\/jats:styled-content>\u2010\u03b1 using an ELISA<\/jats:styled-content>. Inhibition experiments were performed with MAPK<\/jats:styled-content> and NF<\/jats:styled-content>\u03baB inhibitors. SFC<\/jats:styled-content>s were stimulated with IL<\/jats:styled-content>\u20101\u03b2 or TNF<\/jats:styled-content>\u2010\u03b1 after treatment with inhibitors. The MIP<\/jats:styled-content>\u20103\u03b1 levels were measured using an ELISA<\/jats:styled-content>.<\/jats:p><\/jats:sec>Results<\/jats:title>Macrophage inflammatory protein\u20103\u03b1 was the gene most upregulated by IL\u20101\u03b2\u2010 or TNF\u2010\u03b1 stimulation. The mRNA<\/jats:styled-content> and protein levels of MIP\u20103\u03b1 increased in response to IL\u20101\u03b2 in a time\u2010dependent manner. In contrast, during TNF\u2010\u03b1 stimulation, the MIP\u20103\u03b1 mRNA<\/jats:styled-content> levels peaked at 4\u00a0h, and the protein levels peaked at 8\u00a0h. In addition, the IL\u20101\u03b2\u2010 and TNF\u2010\u03b1\u2010stimulated MIP\u20103\u03b1 production was potently reduced by the MAPK and NF\u03baB signaling pathway inhibitors.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Interleukin\u20101\u03b2 and TNF<\/jats:styled-content>\u2010\u03b1 increased the MIP<\/jats:styled-content>\u20103\u03b1 production in SFC<\/jats:styled-content>s via<\/jats:italic> the MAPK<\/jats:styled-content> and NF<\/jats:styled-content>\u03baB pathways. These results suggest that the production of MIP<\/jats:styled-content>\u20103\u03b1 from stimulation with IL<\/jats:styled-content>\u20101\u03b2 or TNF<\/jats:styled-content>\u2010\u03b1 is one factor associated with the inflammatory progression of the internal derangement of the TMJ<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12040","type":"journal-article","created":{"date-parts":[[2013,1,18]],"date-time":"2013-01-18T06:47:00Z","timestamp":1358491620000},"page":"491-498","source":"Crossref","is-referenced-by-count":23,"title":["Effects of interleukin\u20101\u03b2 and tumor necrosis factor\u2010\u03b1 on macrophage inflammatory protein\u20103\u03b1 production in synovial fibroblast\u2010like cells from human temporomandibular joints"],"prefix":"10.1111","volume":"42","author":[{"given":"Miwa","family":"Akutsu","sequence":"first","affiliation":[{"name":"Department of Maxillofacial Surgery Nihon University School of Dentistry at Matsudo Matsudo 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