{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T19:44:49Z","timestamp":1791143089298,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,4,6]],"date-time":"2013-04-06T00:00:00Z","timestamp":1365206400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Fundamental Research Grant Scheme","award":["FP028-2010A"],"award-info":[{"award-number":["FP028-2010A"]}]},{"name":"University of Malaya Research Grant","award":["RG471-12HTM"],"award-info":[{"award-number":["RG471-12HTM"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Epithelial\u2010to\u2010mesenchymal transition (<jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content>) via the mechanism of transcription repression is a crucial process for the induction of invasiveness in many human tumors. Ameloblastoma is a benign odontogenic epithelial neoplasm with a locally infiltrative behavior. Twist, an <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content> promoter, has been implicated in its invasiveness. The roles of the other transcription factors remain unclarified.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Four transcription factors, namely Snail, Slug, <jats:styled-content style=\"fixed-case\">SIP<\/jats:styled-content>1, and Twist, were examined immunohistochemically in 64 ameloblastoma [18 unicystic (<jats:styled-content style=\"fixed-case\">UA<\/jats:styled-content>), 20 solid\/multicystic (<jats:styled-content style=\"fixed-case\">SA<\/jats:styled-content>), 4 desmoplastic (<jats:styled-content style=\"fixed-case\">DA<\/jats:styled-content>), and 22 recurrent (<jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>)].<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All four transcription factors were differentially expressed in ameloblastoma [Snail: <jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>60\/64 (94%); Slug: <jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>21\/64 (33%); <jats:styled-content style=\"fixed-case\">SIP<\/jats:styled-content>:<jats:italic> n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>18\/64 (28%); Twist: <jats:italic>n\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>26\/64 (41%)] (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05). Their distribution patterns were heterogeneous and were not significantly different between the tumor invasive front and central area (<jats:italic>P\u00a0<\/jats:italic>&gt;<jats:italic>\u00a0<\/jats:italic>0.05). Intracellular protein localization was predominantly nuclear for Snail, cytoplasmic&gt;nuclear for Slug and <jats:styled-content style=\"fixed-case\">SIP<\/jats:styled-content>1, and cytoplasmic\/nuclear for Twist. Overexpression of Snail in most subsets (<jats:styled-content style=\"fixed-case\">UA<\/jats:styled-content>\u00a0=\u00a018\/18; <jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>\u00a0=\u00a019\/20; <jats:styled-content style=\"fixed-case\">DA<\/jats:styled-content>\u00a0=\u00a04\/4; <jats:styled-content style=\"fixed-case\">RA<\/jats:styled-content>\u00a0=\u00a019\/22) compared with the other transcription factors (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05) and selective expression for Slug, <jats:styled-content style=\"fixed-case\">SIP<\/jats:styled-content>1, and Twist in squamous\/keratinous foci and at sites of epithelial cystic degeneration were among the main observations made. Stromal cells surrounding immunoreactive tumor cells tended to stain positive.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Present findings suggest that these transcription factors probably play differential roles in mediating local invasiveness in ameloblastoma. Overexpression of Snail in most subsets suggests that this molecule is most likely the prototype transcription factor involved in inducing <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content> in the ameloblastoma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12065","type":"journal-article","created":{"date-parts":[[2013,4,6]],"date-time":"2013-04-06T10:38:41Z","timestamp":1365244721000},"page":"45-52","source":"Crossref","is-referenced-by-count":26,"title":["Differential expression of transcription factors Snail, Slug, <scp>SIP<\/scp>1, and Twist in ameloblastoma"],"prefix":"10.1111","volume":"43","author":[{"given":"Chong","family":"Huat Siar","sequence":"first","affiliation":[{"name":"Department of Oral Pathology Oral Medicine and Periodontology Faculty of Dentistry University of Malaya Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kok Han","family":"Ng","sequence":"additional","affiliation":[{"name":"Formerly Unit of Stomatology Cancer Research Centre Institute for Medical Research Kuala Lumpur Malaysia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,4,6]]},"reference":[{"key":"e_1_2_5_2_1","first-page":"296","volume-title":"World Health Organization Classification of Tumours. 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