{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2024,2,23]],"date-time":"2024-02-23T01:03:39Z","timestamp":1708650219427},"reference-count":42,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,4,17]],"date-time":"2013-04-17T00:00:00Z","timestamp":1366156800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["CA139137"],"award-info":[{"award-number":["CA139137"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Oral cancer in the form of squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content>) is typically detected in advanced stages when treatment is complex and may not be curative. The need for surgical biopsy may contribute to delays in diagnosis and impede early detection. Multiple studies of <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> from surgically obtained tumor samples have revealed many genes differentially expressed with this disease. We sought to determine whether the identified m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s could be used as markers by a non\u2010invasive detection system for <jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content> using <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> from brush cytology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Levels of m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s from 21 genes known to be differentially expressed in head and neck squamous cell carcinoma surgical samples, compared with controls, were shown to be quantifiable in oral brush cytology samples. These m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were quantified in a training set of 14 tumor and 20 non\u2010malignant brush cytology samples from tobacco\/betel nut users. With the measurement of two additional m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and analysis using support vector machines algorithm for class prediction of these cancers was produced.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>This <jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content> classifier based on the levels of 5 m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> from brush cytology initially showed 0.93 sensitivity and 0.91 specificity in differentiating <jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content> from benign oral mucosal lesions based on leave\u2010one\u2010out cross\u2010validation. When used on a test set of 19 samples from 6 <jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content>s and 13 non\u2010malignant oral lesions, we found misclassification of only one <jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content> and one benign lesion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This shows the promise of using <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> from brush cytology for early <jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content> detection and the potential for clinical usage of this non\u2010invasive classifier.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12068","type":"journal-article","created":{"date-parts":[[2013,4,17]],"date-time":"2013-04-17T11:11:55Z","timestamp":1366197115000},"page":"663-669","source":"Crossref","is-referenced-by-count":7,"title":["A prototype tobacco\u2010associated oral squamous cell carcinoma classifier using <scp>RNA<\/scp> from brush cytology"],"prefix":"10.1111","volume":"42","author":[{"given":"Antonia","family":"Kolokythas","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery College of Dentistry University of Illinois at Chicago Chicago IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mitchell J.","family":"Bosman","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Oral Diagnostics Center for Molecular Biology of Oral Diseases University of Illinois at Chicago Chicago IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kristen B.","family":"Pytynia","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology College of Medicine University of Illinois at Chicago Chicago IL USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suchismita","family":"Panda","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Oral Diagnostics Center for 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