{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T18:09:55Z","timestamp":1767895795787,"version":"3.49.0"},"reference-count":32,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2013,4,23]],"date-time":"2013-04-23T00:00:00Z","timestamp":1366675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004886","name":"Cancer Research Foundation in Northern Sweden","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004886","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Lion's Cancer Research Foundation"},{"DOI":"10.13039\/501100002960","name":"V\u00e4sterbotten County Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002960","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100008530","id-type":"DOI","asserted-by":"crossref"}]},{"name":"State Budget of the Czech Republic"},{"name":"RECAMO","award":["CZ.1.05\/2.1.00\/03.0101"],"award-info":[{"award-number":["CZ.1.05\/2.1.00\/03.0101"]}]},{"name":"IGA","award":["NT\/13794-4\/2012"],"award-info":[{"award-number":["NT\/13794-4\/2012"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2014,1]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>p63 proteins are important in formation of the oral mucosa. Normal oral mucosa shows a balance between the six protein isoforms, whereas an imbalance between them is seen in squamous cell carcinomas (<jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>). There is controversy over the clinical impact of p63 in <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>, which may relate to different expression in different areas. In addition, p63 isoforms can act as p53\u2010like molecules (<jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content>p63) or can inhibit p53 functions (\u0394<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>p63) and expression of these isoforms varies in different tumours. Here, we chose to concentrate on the most common intra\u2010oral sub\u2010site, <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content> of the mobile tongue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Total p63, \u0394<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>p63 and <jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content>p63 were analysed separately using immunohistochemistry. The percentage of cells and intensity of expression of different isoforms of p63 was evaluated using a quick score method and correlated with clinical data in a group of 87 patients with tongue <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All tumours expressed p63 in at least 60% of the cells when using two different antibodies detecting all 6 isoforms. p63 expression correlated significantly with 2\u2010year survival (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.018), with fewer patients surviving 2\u00a0years if their tumours expressed p63 with strong intensity in at least 80% of the cells (quick score 18). Looking at 5\u2010year survival, this was even more emphasized. \u0394<jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>p63 was expressed in all tumours, whereas expression of <jats:styled-content style=\"fixed-case\">TA<\/jats:styled-content>p63 was seen only in 59\/87 patients, usually at very low levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Based on the present data, we recommend using expression of p63 as an additional factor contributing prognostic information in analysis of <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content> in the tongue.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12074","type":"journal-article","created":{"date-parts":[[2013,4,23]],"date-time":"2013-04-23T10:27:53Z","timestamp":1366712873000},"page":"14-19","source":"Crossref","is-referenced-by-count":18,"title":["High expression of p63 is correlated to poor prognosis in squamous cell carcinoma of the 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