{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T09:22:51Z","timestamp":1781860971866,"version":"3.54.5"},"reference-count":26,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2013,5,31]],"date-time":"2013-05-31T00:00:00Z","timestamp":1369958400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Functional inactivation of p16 is an early and frequent event in the carcinogenesis of tumours of the head and neck region. However, the prognostic relevance of p16 protein expression for these tumours has been controversial. This study aims to examine immunohistochemical expression of p16 and <jats:styled-content style=\"fixed-case\">HP<\/jats:styled-content> virus in a large number of oral carcinomas, and possible correlation with clinical features of the tumours and survival of the patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Two tissue microarrays composed of 222 oral carcinomas and 427 squamous cell carcinomas of the head and neck region were used for this study. Sections were stained immunohistochemically (anti\u2010p16), and <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> analysis (<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> status) was carried out. Correlation of p16 expression\/<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> status with features of tumours and with survival of the patients was analysed by means of <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>hi\u2010squared test and using <jats:styled-content style=\"fixed-case\">K<\/jats:styled-content>aplan\u2013<jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>eier analysis, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>p16 expression was found immunohistochemically in 74% of tumours, but was not significantly correlated with features of the tumours, but recurrence\u2010free survival of the patients (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.009) if located predominantly nuclear. On the other hand neither intensity of p16 expression (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.41) nor <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> status (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.82) had any effect on these two aspects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Immunohistochemical expression of p16 alone provides a limited tool for diagnosis and prognosis of carcinomas of the head and neck region. Immunohistochemical analysis of p16 depending on its intracellular location might serve as a surrogate marker for <jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content> infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12086","type":"journal-article","created":{"date-parts":[[2013,5,31]],"date-time":"2013-05-31T06:04:13Z","timestamp":1369980253000},"page":"676-681","source":"Crossref","is-referenced-by-count":23,"title":["Immunohistochemical analysis of p16 expression, <scp>HPV<\/scp> infection and its prognostic utility in oral squamous cell carcinoma"],"prefix":"10.1111","volume":"42","author":[{"given":"Alexander","family":"Gr\u00f6be","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henning","family":"Hanken","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lan","family":"Kluwe","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maximilian","family":"Sch\u00f6llchen","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Silke","family":"Tribius","sequence":"additional","affiliation":[{"name":"Department of Radiotherapy and Radio\u2010Oncology University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Pohlenz","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Till","family":"Clauditz","sequence":"additional","affiliation":[{"name":"Department of Pathology University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tobias","family":"Grob","sequence":"additional","affiliation":[{"name":"Department of Pathology University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronald","family":"Simon","sequence":"additional","affiliation":[{"name":"Department of Pathology University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Sauter","sequence":"additional","affiliation":[{"name":"Department of Pathology University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Max","family":"Heiland","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Blessmann","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,5,31]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.27316"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.22355"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.1998.16.3.1197"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434\u2010012\u20102713\u20108"},{"key":"e_1_2_5_6_1","first-page":"979","article-title":"Loss of tumour suppressor p16 expression in initial stages of oral oncogenesis","volume":"27","author":"Vairaktaris E","year":"2007","journal-title":"Anticancer Res"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-0448"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/gyno.1998.5041"},{"key":"e_1_2_5_9_1","first-page":"3445","article-title":"p16 and p53 Protein expression as prognostic indicators of survival and disease recurrence from head and neck cancer","volume":"8","author":"Geisler SA","year":"2002","journal-title":"Clin Cancer Res"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1159\/000096714"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0798-844"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2012.264"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2002.tb01212.x"},{"key":"e_1_2_5_14_1","first-page":"3481","article-title":"p16\/p14(ARF) cell cycle regulatory pathways in primary neuroblastoma: p16 expression is associated with advanced stage disease","volume":"7","author":"Omura\u2010Minamisawa M","year":"2001","journal-title":"Clin Cancer Res"},{"key":"e_1_2_5_15_1","first-page":"544","article-title":"Overexpression of the cell cycle inhibitor p16INK4A in high\u2010grade prostatic intraepithelial neoplasia predicts early relapse in prostate cancer patients","volume":"7","author":"Henshall SM","year":"2001","journal-title":"Clin Cancer Res"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2007.01.010"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-11-3277"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.20872"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.55.1.58"},{"key":"e_1_2_5_20_1","first-page":"1922","article-title":"The expression of p53, p16 proteins and prevalence of apoptosis in oral squamous cell carcinoma. Correlation with mode of invasion grading system","volume":"25","author":"Tokman B","year":"2004","journal-title":"Saudi Med J"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318253a2d1"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2012.07.002"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10006-002-0412-5"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2100"},{"key":"e_1_2_5_25_1","first-page":"2810","article-title":"Cyclin D1 and p16INK4A expression predict reduced survival in carcinoma of the anterior tongue","volume":"5","author":"Bova RJ","year":"1999","journal-title":"Clin Cancer Res"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.21581"},{"key":"e_1_2_5_27_1","first-page":"4619","article-title":"Immunohistochemical expression of the p53, p21\/Waf\u20101, Rb, p16 and Ki67 proteins in multiple myeloma","volume":"20","author":"Kanavaros P","year":"2000","journal-title":"Anticancer Res"}],"container-title":["Journal of Oral Pathology &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12086","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12086","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.12086","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T17:51:49Z","timestamp":1696528309000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jop.12086"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,5,31]]},"references-count":26,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,10]]}},"alternative-id":["10.1111\/jop.12086"],"URL":"https:\/\/doi.org\/10.1111\/jop.12086","archive":["Portico"],"relation":{},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,5,31]]}}}