{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T04:17:04Z","timestamp":1789877824901,"version":"4.0.1"},"reference-count":22,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2013,7,3]],"date-time":"2013-07-03T00:00:00Z","timestamp":1372809600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011842","name":"Centre for International Mobility","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100011842","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Orion-Farmos"},{"DOI":"10.13039\/501100006306","name":"Sigrid Jus\u00e9lius Stiftelse","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100006306","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100002341","name":"Academy of Finland","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002341","id-type":"DOI","asserted-by":"crossref"}]},{"name":"ORTON Orthopaedic Hospital"},{"name":"HUS erva"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2014,2]]},"abstract":"Background<\/jats:title>Recurrent aphthous ulcer (RAU<\/jats:styled-content>) is an ulcerative disease of non\u2010keratinized oral mucosa. Colon and bronchial epithelial cells produce interleukin\u201017C<\/jats:styled-content> (IL<\/jats:styled-content>\u201017C<\/jats:styled-content>) upon stimulation of T<\/jats:styled-content>oll\u2010like receptor 2 (TLR<\/jats:styled-content>2), TLR<\/jats:styled-content>3 and TLR<\/jats:styled-content>5, which are highly expressed in epithelial cells in RAU<\/jats:styled-content> lesions. We therefore investigated the eventual presence and function of IL<\/jats:styled-content>\u201017C<\/jats:styled-content> in cultured human oral keratinocytes (HOK<\/jats:styled-content>) and control biopsies compared to RAU<\/jats:styled-content> lesions.<\/jats:p><\/jats:sec>Methods<\/jats:title>Expression of IL<\/jats:styled-content>\u201017A<\/jats:styled-content>, IL<\/jats:styled-content>\u201017C<\/jats:styled-content>, IL<\/jats:styled-content>\u201017RA<\/jats:styled-content> and IL<\/jats:styled-content>\u201017RE<\/jats:styled-content> was analysed in cultured HOK<\/jats:styled-content> cells using quantitative real\u2010time polymerase chain reaction (qRT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>). HOK<\/jats:styled-content> cells were stimulated with IL<\/jats:styled-content>\u201017C<\/jats:styled-content> and analysed for IL<\/jats:styled-content>\u20108 and tumour necrosis factor\u2010\u03b1 (TNF<\/jats:styled-content>\u2010\u03b1) using qRT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>. Control mucosa (n<\/jats:italic>\u00a0=\u00a05) was immunostained for IL<\/jats:styled-content>\u201017A<\/jats:styled-content>, IL<\/jats:styled-content>\u201017C<\/jats:styled-content>, IL<\/jats:styled-content>\u20108, TNF<\/jats:styled-content>\u2010\u03b1 and mast cell tryptase and compared with RAU<\/jats:styled-content> lesions (n<\/jats:italic>\u00a0=\u00a05) using the mean grey scale value.<\/jats:p><\/jats:sec>Results<\/jats:title>IL<\/jats:styled-content>\u201017C<\/jats:styled-content>, but no IL<\/jats:styled-content>\u201017A<\/jats:styled-content>, mRNA<\/jats:styled-content> was found in cultured HOK<\/jats:styled-content> cells. Components of the heterodimeric IL<\/jats:styled-content>\u201017RA<\/jats:styled-content>\/IL<\/jats:styled-content>\u201017RE<\/jats:styled-content> receptor for IL<\/jats:styled-content>\u201017C<\/jats:styled-content> were also highly expressed. Stimulation of HOK<\/jats:styled-content> with IL<\/jats:styled-content>\u201017C<\/jats:styled-content> increased TNF<\/jats:styled-content>\u2010\u03b1 mRNA<\/jats:styled-content> (P<\/jats:italic>\u00a0=\u00a00.03; IL<\/jats:styled-content>\u20108 increase was not statistically significant). HOK<\/jats:styled-content> in RAU<\/jats:styled-content> lesions stained intensively for IL<\/jats:styled-content>\u201017C<\/jats:styled-content> compared to controls (P<\/jats:italic>\u00a0=\u00a00.006). This was associated with increased epithelial immunostaining of TNF<\/jats:styled-content>\u2010\u03b1 (P<\/jats:italic>\u00a0=\u00a00.04) and IL<\/jats:styled-content>\u20108 (P<\/jats:italic>\u00a0=\u00a00.02). Most of the inflammatory cells which stained for IL<\/jats:styled-content>\u201017A<\/jats:styled-content> in control mucosa and RAU<\/jats:styled-content> lesions were also mast cell tryptase positive.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>IL<\/jats:styled-content>\u201017C<\/jats:styled-content> is highly expressed in epithelial cells in RAU<\/jats:styled-content> lesions, where it seems to stimulate oral keratinocytes via IL<\/jats:styled-content>\u201017RA<\/jats:styled-content>\/IL<\/jats:styled-content>\u201017RE<\/jats:styled-content> to produce pro\u2010inflammatory cytokines. Human oral epithelial cells are probably important inflammatory cells in RAU<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12095","type":"journal-article","created":{"date-parts":[[2013,7,3]],"date-time":"2013-07-03T09:57:45Z","timestamp":1372845465000},"page":"117-124","source":"Crossref","is-referenced-by-count":37,"title":["IL<\/scp>\u201017C<\/scp> and its receptor IL<\/scp>\u201017RA<\/scp>\/IL<\/scp>\u201017RE<\/scp> identify human oral epithelial cell as an inflammatory cell in recurrent aphthous ulcer"],"prefix":"10.1111","volume":"43","author":[{"given":"Ahmed","family":"Al\u2010Samadi","sequence":"first","affiliation":[{"name":"Institute of Clinical Medicine University of Helsinki Helsinki Finland"},{"name":"Institute of Biomedicine University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vesa\u2010Petteri","family":"Kouri","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine University of Helsinki Helsinki Finland"},{"name":"Institute of Biomedicine University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Abdelhakim","family":"Salem","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine University of Helsinki Helsinki Finland"},{"name":"Institute of Biomedicine University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mari","family":"Ainola","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilia","family":"Kaivosoja","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine University of Helsinki Helsinki Finland"},{"name":"School of Electrical Engineering Aalto University Espoo Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gon\u00e7alo","family":"Barreto","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine University of Helsinki Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yrj\u00f6 T.","family":"Konttinen","sequence":"additional","affiliation":[{"name":"Institute of Clinical Medicine University of Helsinki Helsinki Finland"},{"name":"Department of Medicine University of Helsinki and Helsinki University Central Hospital Helsinki Finland"},{"name":"ORTON Orthopaedic Hospital of the Invalid Foundation Helsinki Finland"},{"name":"COXA Hospital for Joint Replacement Tampere Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jarkko","family":"Hietanen","sequence":"additional","affiliation":[{"name":"Institute of Dentistry University of Helsinki Helsinki Finland"},{"name":"HUSLAB Helsinki University Central Hospital Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ritva","family":"H\u00e4yrinen\u2010Immonen","sequence":"additional","affiliation":[{"name":"City of Helsinki Health Care Centre Helsinki Finland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,7,3]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1006\/ijom.2002.0446"},{"key":"e_1_2_5_3_1","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1038\/sj.bdj.4812649","article-title":"Oral medicine\u2010update for the dental practitioner aphthous and other common ulcers","volume":"5","author":"Scully C","year":"2005","journal-title":"Br Dent J"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411980090030401"},{"key":"e_1_2_5_5_1","first-page":"510","article-title":"Immune\u2010inflammatory cells in recurrent oral ulcers (ROU)","volume":"99","author":"H\u00e4yrinen\u2010Immonen R","year":"1991","journal-title":"Scand J Dent Res"},{"key":"e_1_2_5_6_1","first-page":"222","article-title":"Immune\u2010activation in recurrent oral ulcers (ROU)","volume":"1000","author":"H\u00e4yrinen\u2010Immonen R","year":"1992","journal-title":"Scand J Dent Res"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1997.tb00240.x"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0020.2000.290514.x"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1998.tb01927.x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-302X.1994.tb00216.x"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2008.07.009"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-009-0149-7"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2004.08.018"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.25.022106.141557"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2156"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2012-0232OC"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2012.02.008"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni.2155"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2011.01047.x"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2011.01064.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.240671"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1159\/000027650"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1993.tb00116.x"}],"container-title":["Journal of Oral Pathology & Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12095","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12095","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.12095","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T19:25:14Z","timestamp":1696274714000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jop.12095"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,3]]},"references-count":22,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2014,2]]}},"alternative-id":["10.1111\/jop.12095"],"URL":"https:\/\/doi.org\/10.1111\/jop.12095","archive":["Portico"],"relation":{},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,3]]}}}