{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:24:55Z","timestamp":1788870295312,"version":"build-2803163510"},"reference-count":23,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2013,10,11]],"date-time":"2013-10-11T00:00:00Z","timestamp":1381449600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIDCR - DART","award":["T32DE017601"],"award-info":[{"award-number":["T32DE017601"]}]},{"name":"NIDCR - DART","award":["T90DE022736"],"award-info":[{"award-number":["T90DE022736"]}]},{"DOI":"10.13039\/100008333","name":"University of Alabama at Birmingham","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008333","id-type":"DOI","asserted-by":"crossref"}]},{"name":"School of Dentistry Institute of Oral Health Research"},{"name":"UAB Global Center for Craniofacial Oral and Dental Disorders"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2014,3]]},"abstract":"\n \n \n Calcifying epithelial odontogenic tumors (\n CEOT<\/jats:styled-content>\n s) are rare neoplasms derived from dental tissue with the unique characteristic of calcifying amyloid\u2010like material.\n <\/jats:p>\n <\/jats:sec>\n \n Objectives<\/jats:title>\n \n To establish primary\n CEOT<\/jats:styled-content>\n epithelial\u2010derived cell populations, investigate the expression of enamel matrix proteins (\n EMP<\/jats:styled-content>\n s), and identify potential ameloblastin (\n \n AMBN<\/jats:styled-content>\n <\/jats:italic>\n ) and patched 1 (\n \n PTCH<\/jats:styled-content>\n 1\n <\/jats:italic>\n ) gene alterations.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n A 28\u2010year\u2010old patient with a lesion of the posterior maxilla, radiographically characterized by a radiolucency with well\u2010defined borders containing mixed radiopacities, agreed to participate with informed consent. The patient's biopsy confirmed the diagnosis of\n CEOT<\/jats:styled-content>\n , and a small representative tumor fragment was ascertained for cell culture. Explant cultures were established and used to establish primary cell populations. These were analyzed for morphology, cell proliferation, mineralization activity, expression of epithelial\u2010associated markers (\n qRT<\/jats:styled-content>\n \u2010PCR and immunocytochemistry), and gene mutations of\n \n AMBN<\/jats:italic>\n <\/jats:styled-content>\n or\n \n PTCH1<\/jats:italic>\n <\/jats:styled-content>\n .\n DNA<\/jats:styled-content>\n was extracted from tumor cells and gene coding and exon\u2013intron boundaries overlapping fragments amplified.\n PCR<\/jats:styled-content>\n products were bidirectional\n DNA<\/jats:styled-content>\n sequenced and compared against reference sequence.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n A\n CEOT<\/jats:styled-content>\n cell population was established and proliferated in culture and could be maintained for several passages. Expression of\n EMP<\/jats:styled-content>\n s, cytokeratin 14 and 17, and patched (\n \n PTCH1<\/jats:italic>\n <\/jats:styled-content>\n ), as well as\n ALP<\/jats:styled-content>\n activity, was detected. These cells also had the ability to mineralize, similar to the primary tumor. Two\n \n AMBN<\/jats:italic>\n <\/jats:styled-content>\n alterations were identified in the sample: c.1323G>A\/A441A (rs7680880) and c.1344*+111delA. Two single\u2010nucleotide polymorphisms were identified in the\n \n PTCH1<\/jats:italic>\n <\/jats:styled-content>\n gene.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n Our data support the establishment of a\n CEOT<\/jats:styled-content>\n \u2010derived cell population, which expresses known epithelial\u2010associated proteins.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jop.12125","type":"journal-article","created":{"date-parts":[[2013,10,14]],"date-time":"2013-10-14T07:42:15Z","timestamp":1381736535000},"page":"183-190","source":"Crossref","is-referenced-by-count":11,"title":["Establishment and characterization of a primary calcifying epithelial odontogenic tumor cell population"],"prefix":"10.1111","volume":"43","author":[{"given":"Hope M.","family":"Amm","sequence":"first","affiliation":[{"name":"Institute of Oral Health Research University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas L.","family":"Rollins","sequence":"additional","affiliation":[{"name":"Institute of Oral Health Research University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changchun","family":"Ren","sequence":"additional","affiliation":[{"name":"Institute of Oral Health Research University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Dong","sequence":"additional","affiliation":[{"name":"Institute of Oral Health Research University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patricia","family":"DeVilliers","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Rivera","sequence":"additional","affiliation":[{"name":"Head of Oral Pathology Laboratory School of Dentistry Institute of Dental Research Central University of Venezuela Ciudad Universitaria Caracas Venezuela"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary","family":"MacDougall","sequence":"additional","affiliation":[{"name":"Institute of Oral Health Research University of Alabama at Birmingham Birmingham AL USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,10,11]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1368-8375(99)00061-5"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2006.06.050"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-2143(03)00149-5"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2008.11.004"},{"key":"e_1_2_5_6_1","first-page":"3065","article-title":"Mutation of ameloblastin gene in calcifying epithelial odontogenic 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