{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T07:14:00Z","timestamp":1790666040679,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,12,9]],"date-time":"2013-12-09T00:00:00Z","timestamp":1386547200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100012246","name":"Priority Academic Program Development of Jiangsu Higher Education Institutions","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100012246","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>\u03b1\u2010Smooth muscle actin (\u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>)\u2010positive myofibroblasts play a pivotal role in progression and metastasis of solid carcinomas. Epithelial\u2013mesenchymal transition (<jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content>) of cancer cells and lymphogenesis of tumor microenvironment are the important events in tumor metastasis. This study aimed to investigate the relationship between the expression of myofibroblasts marker, \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>, and clinicopathological features, <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content>, lymphogenesis, and prognostic status in oral tongue squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">OTSCC<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Immunohistochemisty was used to detect \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> expression in 50 <jats:styled-content style=\"fixed-case\">OTSCC<\/jats:styled-content>s. <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content> and lymphogenesis were also identified by immunostaining with N\u2010cadherin, vimentin, and lymphatic vessel endothelial hyaluronan receptor 1 (<jats:styled-content style=\"fixed-case\">LYVE<\/jats:styled-content>\u20101).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>There was a significant correlation respectively between the \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.002), vimentin (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.001), N\u2010cadherin (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.025) expression and cervical lymph node metastasis of <jats:styled-content style=\"fixed-case\">OTSCC<\/jats:styled-content>. Carcinomas with \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.001), vimentin (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.003), and N\u2010cadherin (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.012) expression were more advanced in terms of tumor\u2010node\u2010metastases status. Univariate analysis showed that pathologic node status (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001), \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.001), and vimentin expression (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.044) was significantly associated with overall survival time, but multivariate analysis just showed the \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> expression (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.008) and pathologic node status (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.003) was independently predictive of prognosis. Furthermore, statistical analysis showed significant correlation between \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> expression and vimentin (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.037), N\u2010cadherin (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.019), or <jats:styled-content style=\"fixed-case\">LYVE<\/jats:styled-content>\u20101 positive vessel count (<jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.041).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results indicate that \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>\u2010positive myofibroblasts have important impacts on cancer progression, metastasis, and survival prognosis of patients with <jats:styled-content style=\"fixed-case\">OTSCC<\/jats:styled-content>. The functions of \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>\u2010positive myofibroblasts in <jats:styled-content style=\"fixed-case\">OTSCC<\/jats:styled-content> may be associated with promoting <jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content> of tumor cells and lymphogenesis of metastasis microenvironment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12143","type":"journal-article","created":{"date-parts":[[2013,12,9]],"date-time":"2013-12-09T05:51:03Z","timestamp":1386568263000},"page":"335-343","source":"Crossref","is-referenced-by-count":79,"title":["\u03b1\u2010Smooth muscle actin\u2010positive myofibroblasts, in association with epithelial\u2013mesenchymal transition and lymphogenesis, is a critical prognostic parameter in patients with oral tongue squamous cell carcinoma"],"prefix":"10.1111","volume":"43","author":[{"given":"Lei","family":"Ding","sequence":"first","affiliation":[{"name":"Department of Oral and 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