{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T00:45:03Z","timestamp":1780447503923,"version":"3.54.1"},"reference-count":39,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,12,20]],"date-time":"2013-12-20T00:00:00Z","timestamp":1387497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Nihon University Sato"},{"name":"Grant-in-Aid for Scientific Research","award":["#24593066"],"award-info":[{"award-number":["#24593066"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:p>\n                    Acinar cell regeneration from tubular structures has been reported to occur in duct\u2010deligated salivary glands. However, the detailed process of acinar cell regeneration has not been clarified. We have developed a mouse duct ligation model to clarify the mechanisms underlying acinar cell regeneration, and we analyzed the epidermal growth factor receptor (\n                    <jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content>\n                    ) and epidermal growth factor (\n                    <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content>\n                    ) ligands using the model. We studied these ligands expressions in the course of acinar cell regeneration using immunohistochemistry and\n                    <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\n                    \u2010\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    methods. In the duct\u2010ligated portion of the submandibular gland (\n                    <jats:styled-content style=\"fixed-case\">SMG<\/jats:styled-content>\n                    ) that underwent atrophy, newly formed acinar cells were observed arising from the tubular structures after the release of the duct obstruction. The constitutive expression of\n                    <jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content>\n                    was observed by immunohistochemistry in both the duct\u2010ligated and duct\u2010deligated animals as well as in normal controls. The\n                    <jats:styled-content style=\"fixed-case\">EGFR<\/jats:styled-content>\n                    phosphorylation detected on the tubular structures after duct ligation paralleled the acinar cell regeneration.\n                    <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\n                    \u2010\n                    <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\n                    showed an increase in the epiregulin and heparin\u2010binding\n                    <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content>\n                    levels from day 0 to day 3 after the release of the duct obstruction. The\n                    <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content>\n                    level was increased only after day 7.\n                    <jats:italic>In vitro<\/jats:italic>\n                    , cultured cells isolated from ligated\n                    <jats:styled-content style=\"fixed-case\">SMG<\/jats:styled-content>\n                    s proliferated and produced\n                    <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content>\n                    ligands following the addition of epiregulin to the culture medium. These findings suggest that the tubular structures localized in an atrophic gland are the source of acinar cell regeneration of the salivary gland. The induction of\n                    <jats:styled-content style=\"fixed-case\">EGF<\/jats:styled-content>\n                    ligands, in particular epiregulin, may play an important role in acinar cell regeneration in this model.\n                  <\/jats:p>","DOI":"10.1111\/jop.12145","type":"journal-article","created":{"date-parts":[[2013,12,20]],"date-time":"2013-12-20T03:38:34Z","timestamp":1387510714000},"page":"378-387","source":"Crossref","is-referenced-by-count":23,"title":["Epiregulin is critical for the acinar cell regeneration of the submandibular gland in a mouse duct ligation model"],"prefix":"10.1111","volume":"43","author":[{"given":"Koichi","family":"Nagai","sequence":"first","affiliation":[{"name":"Department of Pathology Nihon University School of Dentistry Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hideo","family":"Arai","sequence":"additional","affiliation":[{"name":"Department of Oral Maxillofacial Surgery Nihon University School of Dentistry Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michisato","family":"Okudera","sequence":"additional","affiliation":[{"name":"Department of Pathology Nihon University School of Dentistry Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Yamamura","sequence":"additional","affiliation":[{"name":"Department of Pathology Nihon University School of Dentistry Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidero","family":"Oki","sequence":"additional","affiliation":[{"name":"Department of Oral Maxillofacial Surgery Nihon University School of Dentistry Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazuo","family":"Komiyama","sequence":"additional","affiliation":[{"name":"Department of Pathology Nihon University School of Dentistry Tokyo 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