{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:38:47Z","timestamp":1791319127953,"version":"4.3.1"},"reference-count":27,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T00:00:00Z","timestamp":1392163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>The aim of this study was to study the loss of asymmetrical proliferation in oral tumorigenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>Samples: 92 oral squamous cell carcinomas (<jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content>) with associated non\u2010tumor epithelia (<jats:styled-content style=\"fixed-case\">NTE<\/jats:styled-content>). <jats:styled-content style=\"fixed-case\">NTE<\/jats:styled-content> and tumor were classified as distant from or close to the invasion point. Immunohistochemistry was performed using <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>ib\u20101 antibody. Ki\u201067 was assessed in basal, parabasal layer, medium and upper third, counting total and positive cells. Proliferative patterns were classified according to the ki\u201067 expression: 1\u00a0=\u00a0expression in parabasal layers of well\u2010differentiated tumor nest (<jats:styled-content style=\"fixed-case\">WDTN<\/jats:styled-content>); 2\u00a0=\u00a0expression in parabasal and basal layers of <jats:styled-content style=\"fixed-case\">WDTN<\/jats:styled-content>; 3\u00a0=\u00a0ki\u201067 expression in &lt;20% cells in tumor tissue without <jats:styled-content style=\"fixed-case\">WDTN<\/jats:styled-content>; 4\u00a0=\u00a0ki\u201067 expression in \u226520% of cells in tumor tissue without <jats:styled-content style=\"fixed-case\">WDTN<\/jats:styled-content>; and 5\u00a0= ki\u201067 expression exclusively found in basal layers of <jats:styled-content style=\"fixed-case\">WDTN<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Ki\u201067 expression was highest in parabasal layers of both close and distant <jats:styled-content style=\"fixed-case\">NTE<\/jats:styled-content> (39.7\u00a0\u00b1\u00a027.6 and 30.1\u00a0\u00b1\u00a020) and was also elevated in the close (43.4\u00a0\u00b1\u00a021.3) and distant (48.8\u00a0\u00b1\u00a021.9) tumor tissue samples. Close tumors largely corresponded to proliferation patterns 2 and 4, while distant tumors generally followed pattern 4. Of the 92 close <jats:styled-content style=\"fixed-case\">NTE<\/jats:styled-content> samples, 23 showed reduced basal proliferation with increased parabasal proliferation. Tumors derived from these epithelia followed patterns 2 (52%, 12\/23 cases) or 4 (30.4%, 7\/23 cases). Parabasal proliferation in distant <jats:styled-content style=\"fixed-case\">NTE<\/jats:styled-content> was significantly increased in patients with multiple vs. single tumors (36.7% vs. 25.4%; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.032).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The change from asymmetrical to symmetrical division appears to be an oncogenic mechanism in oral carcinogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12164","type":"journal-article","created":{"date-parts":[[2014,2,12]],"date-time":"2014-02-12T12:47:08Z","timestamp":1392209228000},"page":"507-513","source":"Crossref","is-referenced-by-count":11,"title":["Asymmetrical proliferative pattern loss during malignant transformation of the oral mucosa"],"prefix":"10.1111","volume":"43","author":[{"given":"M. A.","family":"Gonz\u00e1lez\u2010Moles","sequence":"first","affiliation":[{"name":"School of Dentistry Instituto de Biomedicina de Granada University of Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Plaza\u2010Campillo","sequence":"additional","affiliation":[{"name":"School of Dentistry Instituto de Biomedicina de Granada University of Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Ruiz\u2010\u00c1vila","sequence":"additional","affiliation":[{"name":"Unidad de Gesti\u00f3n Cl\u00ednica de Anatom\u00eda Patol\u00f3gica Instituto de biomedicina de Granada Complejo Hospitalario san Cecilio Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Herrera","sequence":"additional","affiliation":[{"name":"School of Dentistry Instituto de Biomedicina de Granada University of Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Bravo","sequence":"additional","affiliation":[{"name":"School of Dentistry Instituto de Biomedicina de Granada University of Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J. A.","family":"Gil\u2010Montoya","sequence":"additional","affiliation":[{"name":"School of Dentistry Instituto de Biomedicina de Granada University of Granada Granada Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,2,12]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1740"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00208-9"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1099513"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-3126"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcancer.2012.03.002"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/35102167"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2499"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2013.04.002"},{"key":"e_1_2_5_10_1","first-page":"525","article-title":"Analysis of Ki\u201067 expression in oral squamous cell carcinoma: why Ki\u201067 is not a prognostic indicator","volume":"46","author":"Gonzalez\u2010Moles MA","year":"2010","journal-title":"Oraloncology"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2009.01611.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.1242"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199009203231205"},{"key":"e_1_2_5_14_1","first-page":"168","volume-title":"World Health Organization classification of tumours pathology and genetics of head and neck tumours","author":"Johnson N","year":"2005"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64266-6"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2004.10.023"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(1950)3:1<32::AID-CNCR2820030106>3.0.CO;2-3"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/157488808783489426"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2011.01845.x"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81631-1"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1095"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-011-0605-0"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2006.01264.x"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2009.12.016"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/bies.201200166"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1218835"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05574"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2007.09.014"}],"container-title":["Journal of Oral Pathology &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12164","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12164","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.12164","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T16:35:43Z","timestamp":1695573343000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jop.12164"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,2,12]]},"references-count":27,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1111\/jop.12164"],"URL":"https:\/\/doi.org\/10.1111\/jop.12164","archive":["Portico"],"relation":{},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2,12]]}}}