{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T19:53:19Z","timestamp":1775073199367,"version":"3.50.1"},"reference-count":24,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,5,9]],"date-time":"2014-05-09T00:00:00Z","timestamp":1399593600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001332","name":"Council for Scientific and Industrial Research","doi-asserted-by":"crossref","award":["09\/716\/(0141\/2011\/EMR-1)"],"award-info":[{"award-number":["09\/716\/(0141\/2011\/EMR-1)"]}],"id":[{"id":"10.13039\/501100001332","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>A small, albeit significant, number of head and neck squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>) patients has no history of tobacco and alcohol use. Such non\u2010habits associated <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>s may represent a distinct clinical entity and exhibit increased aggressiveness. The objective of the study was to understand differences in molecular etiology of habits, and non\u2010habits associated tongue carcinomas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and methods<\/jats:title><jats:p>High\u2010throughput gene expression profiling of 22 tumor samples was carried out. This was followed by quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> validation of four of the identified differentially expressed genes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and conclusion<\/jats:title><jats:p>Eighteen genes were identified that correlate strongly with the habits\u2010 and non\u2010habits distinction. Among the genes significantly overexpressed in the non\u2010habits group are <jats:italic><jats:styled-content style=\"fixed-case\">CCND<\/jats:styled-content>1<\/jats:italic>, a key cell\u2010cycle regulator, <jats:italic><jats:styled-content style=\"fixed-case\">DACT<\/jats:styled-content>3<\/jats:italic>, a modulator of the <jats:styled-content style=\"fixed-case\">W<\/jats:styled-content>nt\/beta\u2010catenin pathway, and three genes associated with the Notch signaling pathway. <jats:italic><jats:styled-content style=\"fixed-case\">CCND<\/jats:styled-content>1<\/jats:italic> and <jats:italic><jats:styled-content style=\"fixed-case\">DACT<\/jats:styled-content>3<\/jats:italic> overexpression in non\u2010habits associated tongue carcinomas were subsequently validated by quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> in an independent cohort (<jats:italic>n<\/jats:italic>\u00a0=\u00a018) of patient samples. Gene expression data were integrated with publicly available protein interaction data to build a small protein interaction network containing five of 18 differentially expressed genes. This suggested that a functional \u2018network module\u2019 can be implicated in the subgroup distinction. All the tumors analyzed here were human papillomavirus (<jats:styled-content style=\"fixed-case\">HPV<\/jats:styled-content>) negative samples. An association between <jats:italic><jats:styled-content style=\"fixed-case\">CCND<\/jats:styled-content>1<\/jats:italic> overexpression in oral tumors and poor prognosis has previously been reported. Thus, <jats:italic><jats:styled-content style=\"fixed-case\">CCND<\/jats:styled-content>1<\/jats:italic> overexpression in non\u2010habits associated anterior tongue carcinomas may contribute to their increased clinical aggressiveness.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12175","type":"journal-article","created":{"date-parts":[[2014,5,9]],"date-time":"2014-05-09T05:21:15Z","timestamp":1399612875000},"page":"593-599","source":"Crossref","is-referenced-by-count":6,"title":["Anterior tongue cancer with no history of tobacco and alcohol use may be a distinct molecular and clinical entity"],"prefix":"10.1111","volume":"43","author":[{"given":"Paul","family":"Sebastian","sequence":"first","affiliation":[{"name":"Division of Surgical Oncology Regional Cancer Centre Thiruvananthapuram India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janki Mohan","family":"Babu","sequence":"additional","affiliation":[{"name":"Cancer Research Program Rajiv Gandhi Centre for Biotechnology Thiruvananthapuram India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"R","family":"Prathibha","sequence":"additional","affiliation":[{"name":"Cancer Research Program Rajiv Gandhi Centre for Biotechnology Thiruvananthapuram India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramkumar","family":"Hariharan","sequence":"additional","affiliation":[{"name":"Cancer Research Program Rajiv Gandhi Centre for Biotechnology Thiruvananthapuram India"},{"name":"RIKEN center for Integrative Medical Sciences Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhavan Radhakrishna","family":"Pillai","sequence":"additional","affiliation":[{"name":"Cancer Research Program Rajiv Gandhi Centre for Biotechnology Thiruvananthapuram India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,5,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2982"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2008.00657.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2006.01274.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.2880160202"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1001\/archotol.1996.01890240021006"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.26195"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-011-0245-3"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.21549"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2008.12.012"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2011.01792.x"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/ng0506-500"},{"key":"e_1_2_5_13_1","doi-asserted-by":"crossref","unstructured":"GouldJ GetzG MontiS ReichM MesirovJP.Comparative gene marker selection suite.Bioinformatics2006;22:1924\u20135.","DOI":"10.1093\/bioinformatics\/btl196"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.286.5439.531"},{"key":"e_1_2_5_15_1","first-page":"baq 020","article-title":"GeneCards Version 3: the human gene integrator","volume":"5","author":"Safran M","year":"2010","journal-title":"Database (Oxford)"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1094"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1916-0216-42-23"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc3090"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2010.03.026"},{"key":"e_1_2_5_20_1","first-page":"8763","article-title":"TIP30 deficiency increases susceptibility to tumorigenesis","volume":"63","author":"Ito M","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M401809200"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.3892\/or_00000565"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2010.00931.x"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1208130"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2011.01861.x"}],"container-title":["Journal of Oral Pathology &amp; 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