{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,3]],"date-time":"2026-06-03T04:50:47Z","timestamp":1780462247099,"version":"3.54.1"},"reference-count":47,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,7,24]],"date-time":"2014-07-24T00:00:00Z","timestamp":1406160000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo-FAPESP","award":["2006\/04264-9"],"award-info":[{"award-number":["2006\/04264-9"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo-FAPESP","award":["2009\/14127-7"],"award-info":[{"award-number":["2009\/14127-7"]}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2014,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Actinic cheilitis (<jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content>) is an oral potentially malignant lesion which is the counterpart of actinic keratosis of the skin and has potential to develop into squamous cell carcinoma. Regulatory T cells (Tregs) have a critical role in modulating the antitumor immune responses. The presence of regulatory T cells in potentially malignant lesions has not been described. We chose investigate the involvement of regulatory T cells in potentially malignant lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The frequency, phenotype, and activity of <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4+<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25+ T cells isolated from blood and lesion of <jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content> patients were analyzed by flow cytometry. Cytokines were quantified by <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>. Data were compared with samples from healthy subjects.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The frequency and suppressor activity of circulating <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4+<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25+ T cells was similar in <jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content> patients and control subjects. However, the frequencies of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010\u2010positive Tregs were higher in <jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content> patients, and these cells inhibited interferon\u2010gamma (<jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3) and increased interleukin (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>)\u201010 productions in co\u2010cultures. Furthermore, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>4+<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>25+ T cells accumulate in <jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content> lesions. Lesions\u2010derived regulatory T cells suppressed lymphocyte proliferation and pro\u2010inflammatory cytokine production. Moreover, high levels of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201010 and transforming growth factor\u2010\u03b2 (<jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b2), and low <jats:styled-content style=\"fixed-case\">IFN<\/jats:styled-content>\u2010\u03b3 were detected in the potentially malignant lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Therefore, our data show that Tregs accumulate in <jats:styled-content style=\"fixed-case\">AC<\/jats:styled-content> lesions, and these cells could be suppressing immune responses in a potentially malignant microenvironment.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12207","type":"journal-article","created":{"date-parts":[[2014,7,24]],"date-time":"2014-07-24T07:20:12Z","timestamp":1406186412000},"page":"754-760","source":"Crossref","is-referenced-by-count":10,"title":["Regulatory T cells in the actinic cheilitis"],"prefix":"10.1111","volume":"43","author":[{"given":"Tha\u00eds Helena","family":"Gasparoto","sequence":"first","affiliation":[{"name":"Department of Biological Sciences Bauru School of Dentistry \u2013 University of S\u00e3o Paulo Bauru Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tatiana Salles","family":"de Souza Malaspina","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Bauru School of Dentistry \u2013 University of S\u00e3o Paulo Bauru Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jos\u00e9 Humberto","family":"Damante","sequence":"additional","affiliation":[{"name":"Department of Stomatology Bauru School of Dentistry \u2013 University of S\u00e3o Paulo Bauru Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Edgard Franco","family":"de Mello","sequence":"additional","affiliation":[{"name":"Lauro de Souza Lima Institute Bauru Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maura Rosane Val\u00e9rio","family":"Ikoma","sequence":"additional","affiliation":[{"name":"Amaral Carvalho Hospital Ja\u00fa Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo Pompermaier","family":"Garlet","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Bauru School of Dentistry \u2013 University of S\u00e3o Paulo Bauru Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Renata Sales Nogueira","family":"Costa","sequence":"additional","affiliation":[{"name":"Lauro de Souza Lima Institute Bauru Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen Ang\u00e9lica","family":"Cavassani","sequence":"additional","affiliation":[{"name":"Department of Pathology Medical School University of Michigan Ann Arbor MC USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jo\u00e3o Santana","family":"da Silva","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Immunology School of Medicine of Ribeir\u00e3o Preto \u2013 University of S\u00e3o Paulo Ribeir\u00e3o Preto Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ana Paula","family":"Campanelli","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences Bauru School of Dentistry \u2013 University of S\u00e3o Paulo Bauru Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,24]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joms.2006.09.016"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1590\/S0365-05962012000100013"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2004.00299.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2004.00242.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-387827-4.00004-8"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-11-2941"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-06-0369"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.7150\/ijbs.7.651"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.25429"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2008.04.008"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgs103"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-1574"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-1681"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-4217"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0408197102"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.194.5.629"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2009.03550.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1086\/502980"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humimm.2013.08.269"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni904"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.24.021605.090737"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200423395"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.2.834"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1180"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.192.2.303"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri1806"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-009-0803-7"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2010.09.002"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2012.00030"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2013.00106"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070104"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2097"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI65180"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2742"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1001-1118"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2012.04657.x"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1186\/1479-5876-11-148"},{"key":"e_1_2_5_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10875-007-9076-0"},{"key":"e_1_2_5_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/ni1102-999"},{"key":"e_1_2_5_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2011.06.005"},{"key":"e_1_2_5_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.306"},{"key":"e_1_2_5_43_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.8456301"},{"key":"e_1_2_5_44_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.62.4.450"},{"key":"e_1_2_5_45_1","first-page":"2426","article-title":"Multiple roles of interferon\u2010\u03b3 in the mediation of interleukin 12\u2010induced tumor regression","volume":"58","author":"Ogawa M","year":"1998","journal-title":"Cancer Res"},{"key":"e_1_2_5_46_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2037"},{"key":"e_1_2_5_47_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(12)70582-X"},{"key":"e_1_2_5_48_1","doi-asserted-by":"publisher","DOI":"10.5021\/ad.2011.23.1.101"}],"container-title":["Journal of Oral Pathology &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12207","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12207","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.12207","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,5]],"date-time":"2023-10-05T18:21:59Z","timestamp":1696530119000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jop.12207"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,7,24]]},"references-count":47,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2014,11]]}},"alternative-id":["10.1111\/jop.12207"],"URL":"https:\/\/doi.org\/10.1111\/jop.12207","archive":["Portico"],"relation":{},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,7,24]]}}}