{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T19:09:04Z","timestamp":1759777744901},"reference-count":29,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,7,28]],"date-time":"2014-07-28T00:00:00Z","timestamp":1406505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Prevention and early diagnosis have the greatest potential for public health and are the most effective method in the long\u2010term to control oral cancer. The aim was to apply <jats:styled-content style=\"fixed-case\">PAP<\/jats:styled-content> staining together with Ag<jats:styled-content style=\"fixed-case\">NOR<\/jats:styled-content> staining and morphometric analysis in oral exfoliative cytology, to determine the sensitivity and specificity of these methods in the detection of malignant changes for the purposes of both initial population monitoring and follow\u2010up.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ag<jats:styled-content style=\"fixed-case\">NOR<\/jats:styled-content>, Papanicolau, and morphometric tests were conducted in samples of patients with oral cancer, oral potentially malignant disorders and controls (opposite side of lesions). Specificity and sensitivity values for each stain method and the curve under <jats:styled-content style=\"fixed-case\">ROC<\/jats:styled-content> area were estimated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The diagnostic variables which allowed greatest accuracy in identifying malignancy relative to the healthy control were cluster (76.92%), satellite (75.64%), and total (90%). The diagnosis was seen to be associated with <jats:styled-content style=\"fixed-case\">PAP<\/jats:styled-content> and total Ag<jats:styled-content style=\"fixed-case\">NOR<\/jats:styled-content>, total Ag<jats:styled-content style=\"fixed-case\">NOR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">PAP<\/jats:styled-content>, total Ag<jats:styled-content style=\"fixed-case\">NOR<\/jats:styled-content> and satellites and clusters, and total Ag<jats:styled-content style=\"fixed-case\">NOR<\/jats:styled-content> nuclear area\/cytoplasmic area ratio.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The total number of Ag<jats:styled-content style=\"fixed-case\">NOR<\/jats:styled-content> is a reliable marker for detecting neoplastic cells; this method increases sensitivity and specificity by decreasing the likelihood of false negatives or positives, as the accuracy obtained was 90%. It is also a low\u2010cost, non\u2010invasive, simple methodology that can be recommended to help the early detection of oral cancer and monitoring of patients with a first diagnosis of cancer.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12219","type":"journal-article","created":{"date-parts":[[2014,7,28]],"date-time":"2014-07-28T05:11:49Z","timestamp":1406524309000},"page":"178-184","source":"Crossref","is-referenced-by-count":14,"title":["Exfoliative cytology as a tool for monitoring pre\u2010malignant and malignant lesions based on combined stains and morphometry techniques"],"prefix":"10.1111","volume":"44","author":[{"given":"Ignacio","family":"Gonzalez Segura","sequence":"first","affiliation":[{"name":"Facultad de Odontolog\u00eda Universidad Nacional de C\u00f3rdoba C\u00f3rdoba Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dante","family":"Secchi","sequence":"additional","affiliation":[{"name":"Facultad de Odontolog\u00eda Universidad Nacional de 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