{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,8]],"date-time":"2026-03-08T23:41:48Z","timestamp":1773013308344,"version":"3.50.1"},"reference-count":59,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,9,12]],"date-time":"2014-09-12T00:00:00Z","timestamp":1410480000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["303075839"],"award-info":[{"award-number":["303075839"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2015,1]]},"abstract":"Background<\/jats:title>Little is known about mesenchymal stem cells (MSC<\/jats:styled-content>s) in normal or inflammatory oral mucosal tissues, such as in oral lichen planus (OLP<\/jats:styled-content>). Our objectives were to identify, isolate, and characterize MSC<\/jats:styled-content>s from normal human oral mucosa and OLP<\/jats:styled-content> lesions, and to evaluate indoleamine 2,3 dioxygenase (IDO<\/jats:styled-content>) activity in mediating immunomodulation of MSC<\/jats:styled-content>s from these tissues.<\/jats:p><\/jats:sec>Methods<\/jats:title>Expressions of MSC<\/jats:styled-content>s\u2010related markers were examined in isolated cells by flow cytometry. Self\u2010renewal and multilineage differentiations were studied to characterize these MSC<\/jats:styled-content>s. Interferon\u2010\u03b3 (IFN<\/jats:styled-content>\u2010\u03b3), IDO<\/jats:styled-content>, and STRO<\/jats:styled-content>\u20101 were assessed by immunofluorescence. MSC<\/jats:styled-content>s from oral mucosa and OLP<\/jats:styled-content> or IFN<\/jats:styled-content>\u2010\u03b3\u2010pretreated MSC<\/jats:styled-content>s were co\u2010cultured with allogeneic mixed lymphocyte reaction assays (MLR<\/jats:styled-content>). Proliferation and apoptosis of MLR<\/jats:styled-content> or MSC<\/jats:styled-content>s were detected by CCK<\/jats:styled-content>8 and the annexin V\u2010FITC<\/jats:styled-content> apoptosis detection kit, respectively. IDO<\/jats:styled-content> expression and activity were measured by real\u2010time PCR<\/jats:styled-content>, Western blotting, and high\u2010performance liquid chromatography.<\/jats:p><\/jats:sec>Results<\/jats:title>Isolated cells from oral mucosa and OLP<\/jats:styled-content> expressed MSC<\/jats:styled-content>\u2010related markers STRO<\/jats:styled-content>\u20101, CD<\/jats:styled-content>105, and CD<\/jats:styled-content>90 but were absent for hematopoietic stem cell markers CD<\/jats:styled-content>34. Besides, they all showed self\u2010renewal and multilineage differentiation capacities. MSC<\/jats:styled-content>s in OLP<\/jats:styled-content> presented STRO<\/jats:styled-content>\u20101\/IDO<\/jats:styled-content>+ phenotype by immunofluorescence. MSC<\/jats:styled-content>s and IFN<\/jats:styled-content>\u2010\u03b3\u2010pretreated MSC<\/jats:styled-content>s could inhibit lymphocyte proliferation via IDO<\/jats:styled-content> activity, but not via cell apoptosis. Long\u2010term IFN<\/jats:styled-content>\u2010\u03b3 could also inhibit MSC<\/jats:styled-content> proliferation via IDO<\/jats:styled-content> activity.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Mesenchymal stem cells can be isolated from human oral mucosa and OLP<\/jats:styled-content> tissues. Besides self\u2010renewal and multilineage differentiation properties, these cells may participate in immunomodulation mediated by IFN<\/jats:styled-content>\u2010\u03b3 via IDO<\/jats:styled-content> activity in human OLP<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12224","type":"journal-article","created":{"date-parts":[[2014,9,12]],"date-time":"2014-09-12T08:20:48Z","timestamp":1410510048000},"page":"15-27","source":"Crossref","is-referenced-by-count":22,"title":["Interferon\u2010\u03b3 regulates the function of mesenchymal stem cells from oral lichen planus via indoleamine 2,3\u2010dioxygenase activity"],"prefix":"10.1111","volume":"44","author":[{"given":"Zhihui","family":"Zhang","sequence":"first","affiliation":[{"name":"School and Hospital of Stomatology Peking University Beijing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying","family":"Han","sequence":"additional","affiliation":[{"name":"School and Hospital of Stomatology 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