{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T22:23:37Z","timestamp":1778711017952,"version":"3.51.4"},"reference-count":55,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2014,8,28]],"date-time":"2014-08-28T00:00:00Z","timestamp":1409184000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2015,3]]},"abstract":"<jats:sec><jats:title>Purpose<\/jats:title><jats:p>The aim of this study was to evaluate whether sleep restriction (<jats:styled-content style=\"fixed-case\">SR<\/jats:styled-content>) could affect the mechanisms and pathways\u2019 essentials for cancer cells in tongue cancer induced by 4\u2010nitroquinoline 1\u2010oxide in Wistar rats.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The animals were distributed into 4 groups of 5 animals each treated with 50\u00a0ppm 4 <jats:styled-content style=\"fixed-case\">NQO<\/jats:styled-content> solution through their drinking water for 4 and 12\u00a0weeks. The animals were submitted to sleep restriction for 21\u00a0days using the modified multiple platform method, which consisted of placing 5 rats in a cage (41\u00a0\u00d7\u00a034\u00a0\u00d7\u00a016\u00a0cm) containing 10 circular platforms (3.5\u00a0cm in diameter) with water 1\u00a0cm below the upper surface. The investigations were conducted using immunohistochemistry of p53, Bax and Bcl\u20102 proteins related to apoptosis and its pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Although no histopathologic abnormalities were induced in the epithelium after 4\u00a0weeks of carcinogen exposure in all groups, in 12\u00a0weeks were observed pre\u2010neoplastic lesions. Data analysis revealed statistically significant differences (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.05) in 4\u00a0weeks group for p53, and for bcl\u20102. Following 12\u00a0weeks of 4<jats:styled-content style=\"fixed-case\">NQO<\/jats:styled-content> administration, we found significant differences between <jats:styled-content style=\"fixed-case\">SR<\/jats:styled-content> and control groups in p53, bax, and bcl\u20102 immunoexpression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results reveal that sleep restriction exerted alterations in proteins associated with proliferation and apoptosis in carcinogenesis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12225","type":"journal-article","created":{"date-parts":[[2014,8,28]],"date-time":"2014-08-28T16:25:56Z","timestamp":1409243156000},"page":"222-228","source":"Crossref","is-referenced-by-count":6,"title":["The influence of sleep restriction on expression of apoptosis regulatory proteins p53, Bcl\u20102 and Bax following rat tongue carcinogenesis induced by 4\u2010nitroquinoline 1\u2010oxide"],"prefix":"10.1111","volume":"44","author":[{"given":"Juliana","family":"Noguti","sequence":"first","affiliation":[{"name":"Departamento de Patologia Universidade Federal de S\u00e3o Paulo UNIFESP S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tathiana A.","family":"Alvarenga","sequence":"additional","affiliation":[{"name":"Departamento de Psicobiologia Universidade Federal de S\u00e3o Paulo UNIFESP S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patr\u00edcia","family":"Marchi","sequence":"additional","affiliation":[{"name":"Departamento de Patologia Universidade Federal de S\u00e3o Paulo UNIFESP S\u00e3o Paulo SP Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Celina T. 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