{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T16:10:09Z","timestamp":1782403809739,"version":"3.54.5"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,7,22]],"date-time":"2014-07-22T00:00:00Z","timestamp":1405987200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81361120399"],"award-info":[{"award-number":["81361120399"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81272961"],"award-info":[{"award-number":["81272961"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81372891"],"award-info":[{"award-number":["81372891"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"crossref","award":["81321002"],"award-info":[{"award-number":["81321002"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Fundamental Research Funds of the Central Universities of China"},{"name":"State Key Laboratory of Oral Diseases Special Funded Projects"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2015,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Hyperthermia has been shown promising in the treatment of head and neck squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>); however, the mechanism underlying hyperthermia reducing tumor metastasis is poorly elucidated. <jats:styled-content style=\"fixed-case\">TWIST<\/jats:styled-content>2, an important transcription factor of epithelial\u2010mesenchymal transition (<jats:styled-content style=\"fixed-case\">EMT<\/jats:styled-content>), plays a critical role in the tumor progression and metastasis. The role of <jats:styled-content style=\"fixed-case\">TWIST<\/jats:styled-content>2 in tongue squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>) and its association with hyperthermia still have not been reported.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>The correlations between <jats:styled-content style=\"fixed-case\">TWIST<\/jats:styled-content>2 expression and the clinical\u2010pathologic characteristics of 89 patients with <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> were evaluated by immunohistochemical staining. <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> cell lines transfected with si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> against <jats:styled-content style=\"fixed-case\">TWIST<\/jats:styled-content>2 were heated for 40\u00a0min at 42.5\u00b0C, and the migration capability of cells was examined by migration assay. Xenograft tumors in nude mice were treated by hyperthermia, and <jats:styled-content style=\"fixed-case\">TWIST<\/jats:styled-content>2 expression was measured.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our data showed that <jats:styled-content style=\"fixed-case\">TWIST<\/jats:styled-content>2 expression was associated with the metastasis of human <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>. In Tca8113 and Cal\u201027 cells, <jats:styled-content style=\"fixed-case\">TWIST<\/jats:styled-content>2\u2010si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> treatment can reduce cell migration ability and has no effect on the cell proliferation and apoptosis. Hyperthermia can decrease the level of <jats:styled-content style=\"fixed-case\">TWIST<\/jats:styled-content>2 in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> and inhibit the migration of cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>This demonstrated that hyperthermia might decrease the migration of Tca8113 and Cal\u201027 cells by reducing <jats:styled-content style=\"fixed-case\">TWIST<\/jats:styled-content>2 expression. Altogether, these findings suggest an as yet undescribed link between <jats:styled-content style=\"fixed-case\">TWIST<\/jats:styled-content>2 and hyperthermia in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12237","type":"journal-article","created":{"date-parts":[[2014,7,22]],"date-time":"2014-07-22T06:09:32Z","timestamp":1406009372000},"page":"337-344","source":"Crossref","is-referenced-by-count":10,"title":["Hyperthermia inhibited the migration of tongue squamous cell carcinoma through <scp>TWIST<\/scp>2"],"prefix":"10.1111","volume":"44","author":[{"given":"Ya\u2010ling","family":"Tang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Oral Diseases West China Hospital of Stomatology (Sichuan University) Chengdu Sichuan China"},{"name":"Department of Oral Pathology West China Hospital of Stomatology (Sichuan University) Chengdu Sichuan 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