{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,9]],"date-time":"2026-07-09T14:45:22Z","timestamp":1783608322966,"version":"3.55.0"},"reference-count":55,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,9,22]],"date-time":"2014-09-22T00:00:00Z","timestamp":1411344000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2015,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Juvenile idiopathic arthritis (<jats:styled-content style=\"fixed-case\">JIA<\/jats:styled-content>) of the temporomandibular joint (<jats:styled-content style=\"fixed-case\">TMJ<\/jats:styled-content>) can cause severe growth disturbances of the craniomandibular system. Antigen\u2010induced arthritis (<jats:styled-content style=\"fixed-case\">AIA<\/jats:styled-content>) of the rabbit <jats:styled-content style=\"fixed-case\">TMJ<\/jats:styled-content> is simulating the inflammatory process of the <jats:styled-content style=\"fixed-case\">TMJ<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">JIA<\/jats:styled-content>. The aim of this study was to investigate the effect of a systemic administration of methotrexate (<jats:styled-content style=\"fixed-case\">MTX<\/jats:styled-content>) on <jats:styled-content style=\"fixed-case\">AIA<\/jats:styled-content> in rabbits by means of three different histological staining methods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>After sensitization, a bilateral arthritis of the <jats:styled-content style=\"fixed-case\">TMJ<\/jats:styled-content> was induced by an intra\u2010articular administration of ovalbumin in 12 <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>ew <jats:styled-content style=\"fixed-case\">Z<\/jats:styled-content>ealand white rabbits aged 10\u00a0weeks. From the 13th week of age, six of the 12 rabbits received weekly intramuscular injections of <jats:styled-content style=\"fixed-case\">MTX<\/jats:styled-content>, and the other six animals remained without therapy. Another six animals served as controls, receiving no treatment or intra\u2010articular injections at all. After euthanasia at the age of 22\u00a0weeks, all <jats:styled-content style=\"fixed-case\">TMJ<\/jats:styled-content>s were retrieved en bloc. Sagittal sections were cut and stained with haematoxylin\u2013eosin (<jats:styled-content style=\"fixed-case\">H<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">E<\/jats:styled-content>), <jats:styled-content style=\"fixed-case\">S<\/jats:styled-content>afranin\u2010<jats:styled-content style=\"fixed-case\">O<\/jats:styled-content> for the evaluation of the <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>ankin score and tartrate\u2010resistant acid phosphatase (<jats:styled-content style=\"fixed-case\">TRAP<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In the arthritis group, a chronic inflammation with degeneration of the articular cartilage was visible. In the <jats:styled-content style=\"fixed-case\">MTX<\/jats:styled-content> group, the signs of cartilage degeneration were significantly reduced compared with the arthritis group. In contrast, the joints in the control group were inconspicuous. A correlation between the <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>ankin score and <jats:styled-content style=\"fixed-case\">TRAP<\/jats:styled-content>\u2010positive cells could be found.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Systemic administration of <jats:styled-content style=\"fixed-case\">MTX<\/jats:styled-content> seems to have a positive effect upon the inflammatory process in the rabbit <jats:styled-content style=\"fixed-case\">TMJ<\/jats:styled-content> but fails to eliminate the sign of arthritis completely.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12265","type":"journal-article","created":{"date-parts":[[2014,9,22]],"date-time":"2014-09-22T06:58:22Z","timestamp":1411369102000},"page":"614-621","source":"Crossref","is-referenced-by-count":11,"title":["Effect of methotrexate upon antigen\u2010induced arthritis of the rabbit temporomandibular joint"],"prefix":"10.1111","volume":"44","author":[{"given":"Smbat","family":"Rafayelyan","sequence":"first","affiliation":[{"name":"Department of Orthodontics, Dentofacial Orthopedics and Pedodontics Center for Dental and Craniofacial Sciences Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philipp","family":"Meyer","sequence":"additional","affiliation":[{"name":"Department of Orthodontics, Dentofacial Orthopedics and Pedodontics Center for Dental and Craniofacial Sciences Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ralf J.","family":"Radlanski","sequence":"additional","affiliation":[{"name":"Department of Oral Structural Biology Center for Dental and Craniofacial Sciences Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin Berlin 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