{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,3]],"date-time":"2026-03-03T12:59:51Z","timestamp":1772542791755,"version":"3.50.1"},"reference-count":14,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2014,9,25]],"date-time":"2014-09-25T00:00:00Z","timestamp":1411603200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Nature Science Foundation of China","award":["81271137"],"award-info":[{"award-number":["81271137"]}]},{"name":"Lundbeck Foundation - Lundbeck Foundation Nanomedicine Center for Individualized Management of Tissue Damage and Regeneration"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2015,8]]},"abstract":"<jats:p>We established a murine periodontitis model by local injection of lipopolysaccharide of <jats:italic>Porphyromonas gingivalis (Pg\u2010<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content>)<\/jats:italic> into the gingival sulcus of mandibular left incisor four times with 48\u2010h interval. The histological examination of the periodontal tissues demonstrated that significant loss of periodontal bone and ligaments was observed in the lesion side with abundant inflammatory cell infiltration. Two days after the last injection, Cy5\u2010labelled si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\/chitosan particles were injected intraperitoneally (ip). The chitosan\/si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> particles were taken up by peritoneal macrophages, which subsequently migrated to the inflamed gingival area evaluated by <jats:italic>in vivo<\/jats:italic> imaging. The localization of macrophages in the inflamed region was further confirmed by immunofluorescent staining. The present report demonstrates that intragingival injection of <jats:italic>Pg\u2010<jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content><\/jats:italic> can be used to create an experimental model of periodontal inflammation in mice and that recruitment of macrophages with chitosan\/si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> nanoparticles to the inflamed area opens the possibility of an <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>i\u2010based therapeutic approach using chitosan as a carrier in periodontitis.<\/jats:p>","DOI":"10.1111\/jop.12269","type":"journal-article","created":{"date-parts":[[2014,9,25]],"date-time":"2014-09-25T14:13:36Z","timestamp":1411654416000},"page":"538-542","source":"Crossref","is-referenced-by-count":12,"title":["Macrophage\u2010mediated nanoparticle delivery to the periodontal lesions in established murine model <i>via<\/i> Pg\u2010<scp>LPS<\/scp> induction"],"prefix":"10.1111","volume":"44","author":[{"given":"Zhiwei","family":"Ma","sequence":"first","affiliation":[{"name":"State Key Laboratory of Military Stomatology Department of Periodontology School of Stomatology The Fourth Military Medical University Xi\u2010an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frederik","family":"Dagn\u00e6s\u2010Hansen","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Aarhus University Aarhus C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Henrik","family":"L\u00f8vschall","sequence":"additional","affiliation":[{"name":"Department of Dentistry, Health Aarhus University Aarhus C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wen","family":"Song","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Military Stomatology Department of Prothodontics School of Stomatology The Fourth Military Medical University Xi\u2010an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gitte K.","family":"Nielsen","sequence":"additional","affiliation":[{"name":"Department of Biomedicine Aarhus University Aarhus C Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuanxu","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Interdisciplinary Nanoscience Center (iNANO) Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qintao","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Military Stomatology Department of Periodontology School of Stomatology The Fourth Military Medical University Xi\u2010an China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J\u00f8rgen","family":"Kjems","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Interdisciplinary Nanoscience Center (iNANO) Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shan","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Molecular Biology and Genetics Interdisciplinary Nanoscience Center (iNANO) Aarhus University Aarhus Denmark"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,9,25]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67728-8"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1155\/2011\/754857"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2009.01393.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2009.01207.x"},{"issue":"1","key":"e_1_2_6_6_1","first-page":"19","article-title":"Biological activities of lipopolysaccharides from oral bacteria and their relevance to the pathogenesis of chronic periodontitis","volume":"78","author":"Wilson M","year":"1995","journal-title":"Sci Prog"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12023"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.5051\/jpis.2013.43.4.147"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2008.220"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.radonc.2010.09.010"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.3109\/0284186X.2012.726373"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2004.00528.x"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.7150\/thno.5743"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1177\/41.9.7689084"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-009-1031-x"}],"container-title":["Journal of Oral Pathology &amp; 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