{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T07:53:47Z","timestamp":1740124427816,"version":"3.37.3"},"reference-count":68,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2015,11,2]],"date-time":"2015-11-02T00:00:00Z","timestamp":1446422400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100011913","name":"Kaohsiung Veterans General Hospital","doi-asserted-by":"crossref","award":["VGHKS103-007","VGHKS104-050","VGHKS104-051"],"award-info":[{"award-number":["VGHKS103-007","VGHKS104-050","VGHKS104-051"]}],"id":[{"id":"10.13039\/501100011913","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2016,7]]},"abstract":"<jats:sec><jats:title>Backgrounds<\/jats:title><jats:p>Oral cancer is the 4th leading cause of cancer death for males and the top cancer in young adult males in Taiwan. Tongue squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>) is a common oral cancer and generally associated with poor prognosis. Global <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> hypomethylation at the 5 position of cytosine (5mC) is a well\u2010known epigenetic feature of cancer. Therefore, the purpose of this study was to investigate the relationship of the global 5mC content with the tumorigenesis and prognosis of patients with <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The levels of global 5mC were evaluated by immunohistochemistry using tissue microarray slides of 248 surgically resected <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> and 202 corresponding tumor adjacent normal (<jats:styled-content style=\"fixed-case\">TAN<\/jats:styled-content>) tissues.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that the level of 5mC in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> (<jats:italic>P<\/jats:italic> &lt; 0.001) was significantly decreased as compared to <jats:styled-content style=\"fixed-case\">TAN<\/jats:styled-content>. Among <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> tissues, decreased levels of 5mC were associated with female gender (<jats:italic>P<\/jats:italic> = 0.036). In addition, the global hypomethylation was associated with the poor disease\u2010specific survival in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> patients (adjusted hazard ratio: 1.55, <jats:italic>P<\/jats:italic> = 0.043), especially for patients in older age group (&gt; 50 years, <jats:italic>P<\/jats:italic> = 0.013), with moderate or poor cell differentiation (<jats:italic>P<\/jats:italic> = 0.044), early stage of disease (I\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>,<jats:italic> P<\/jats:italic> = 0.046), small tumor size (T1\u2013T2, <jats:italic>P<\/jats:italic> = 0.005), without lymph node involvement (<jats:italic>P<\/jats:italic> = 0.041), and ever received postoperative radiotherapy (<jats:italic>P<\/jats:italic> = 0.009).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Global hypomethylation was an independent biomarker for the development and poor prognosis of <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12381","type":"journal-article","created":{"date-parts":[[2015,11,3]],"date-time":"2015-11-03T04:51:59Z","timestamp":1446526319000},"page":"409-417","source":"Crossref","is-referenced-by-count":18,"title":["Global <scp>DNA<\/scp> hypomethylation is associated with the development and poor prognosis of tongue squamous cell carcinoma"],"prefix":"10.1111","volume":"45","author":[{"given":"Hung\u2010Chih","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Stomatology Kaohsiung Veterans General Hospital Kaohsiung Taiwan"},{"name":"Department of Dental Technology Shu\u2010Zen Junior College of Medicine and Management Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheng\u2010Mei","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Stomatology Kaohsiung Veterans General Hospital Kaohsiung Taiwan"},{"name":"Department of Dental Technology Shu\u2010Zen Junior College of Medicine and Management Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jiin\u2010Tsuey","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Biological Sciences National Sun Yat\u2010Sen University Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuo\u2010Wang","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Medical Education and Research Kaohsiung Veterans General Hospital Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ting\u2010Ying","family":"Fu","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine Kaohsiung Veterans General Hospital Kaohsiung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Huei\u2010Han","family":"Liou","sequence":"additional","affiliation":[{"name":"Department of Medical Education and Research 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