{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T20:35:01Z","timestamp":1790800501815,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,12,29]],"date-time":"2015-12-29T00:00:00Z","timestamp":1451347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81302358"],"award-info":[{"award-number":["81302358"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100007219","name":"Natural Science Foundation of Shanghai","doi-asserted-by":"publisher","award":["12ZR1417000"],"award-info":[{"award-number":["12ZR1417000"]}],"id":[{"id":"10.13039\/100007219","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Oral lichen planus (<jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content>) is a T cell\u2010mediated autoimmune disease involving oral mucosa. Interleukin\u201022 (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022) as the signature cytokine of T helper 22 cells is increasingly recognized as a key regulator in various autoimmune diseases. Our previous study reported that <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 immunoexpression in <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content> was significantly increased compared with the normal controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The objective of this preliminary study was to compare the <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 expression levels in oral biopsies from patients with <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content> (<jats:italic>n<\/jats:italic> = 50) against normal oral mucosa (<jats:italic>n<\/jats:italic> = 19) using <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> and Western blot, identify the potential targeting mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022, and examine the mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression levels in <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Interleukin\u201022 expression level in <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content> was significantly increased compared with the normal controls. The Dual\u2010Luciferase reporter assay system in human embryonic kidney 293 (<jats:styled-content style=\"fixed-case\">HEK<\/jats:styled-content>293) cells demonstrated that miR\u2010562 and miR\u2010203 were the target mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022, which was consistent with predictions from bioinformatics software analyses. Interestingly, miR\u2010562 expression in <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content> was significantly decreased, but miR\u2010203 expression in <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content> was significantly increased compared with the normal controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This preliminary study for the first time reported that aberrant expression levels of miR\u2010562 and miR\u2010203 were associated with high expression of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 and demonstrated the target relationship between mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 in <jats:styled-content style=\"fixed-case\">HEK<\/jats:styled-content>293 cells. Our data indicated that <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 and its targeting mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s contribute to the pathogenesis of <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content>. Further studies are required to investigate the regulatory pathways of <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u201022 and miR\u2010562 and miR\u2010203 in <jats:styled-content style=\"fixed-case\">OLP<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12404","type":"journal-article","created":{"date-parts":[[2015,12,29]],"date-time":"2015-12-29T09:17:09Z","timestamp":1451380629000},"page":"523-527","source":"Crossref","is-referenced-by-count":15,"title":["Aberrant expression of interleukin\u201022 and its targeting micro<scp>RNA<\/scp>s in oral lichen planus: a preliminary study"],"prefix":"10.1111","volume":"45","author":[{"given":"Zhengyu","family":"Shen","sequence":"first","affiliation":[{"name":"Department of Dermatology Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guanhuan","family":"Du","sequence":"additional","affiliation":[{"name":"Department of Oral Mucosal Diseases Shanghai Key Laboratory of Stomatology Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zengtong","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Oral Mucosal Diseases Shanghai Key Laboratory of Stomatology Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Oral Mucosal Diseases Shanghai Key Laboratory of Stomatology Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Linjun","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Oral Mucosal Diseases Shanghai Key Laboratory of Stomatology Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Dermatology Ninth People's Hospital Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2015,12,29]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2012.01953.x"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.3109\/08923973.2010.498014"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2010.00946.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2013.04.004"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.2013.736497"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jdermsci.2013.04.028"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1155\/2014\/813926"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrg1379"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.06.017"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2010.06.005"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1601-0825.2011.01869.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2011.01063.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2011.01084.x"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0063015"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00403-011-1197-x"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0714.2003.00125.x"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12168"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4176"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2013.11.008"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/801974"},{"key":"e_1_2_5_23_1","doi-asserted-by":"crossref","first-page":"970607","DOI":"10.1155\/2014\/970607","article-title":"Mechanisms of iRNA\u2010mediated gene regulation from common downregulation to mRNA\u2010specific upregulation","volume":"2014","author":"Valinezhad Orang A","year":"2014","journal-title":"Int J Genomics"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2012.07.031"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000610"}],"container-title":["Journal of Oral Pathology &amp; 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