{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T12:28:00Z","timestamp":1790857680617,"version":"4.1.0"},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2016,1,13]],"date-time":"2016-01-13T00:00:00Z","timestamp":1452643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81302356"],"award-info":[{"award-number":["81302356"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2016,8]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>TGF\u03b21 and Smad3 play an important role in the process of EMT. TGF\u03b21 regulates the expression of Jagged1 by modulating Notch signaling. Jagged1 is related to tumor invasion, metastasis, chemotherapy resistance, and tumor immune escape. The aims of this study are to examine deregulation of TGF\u03b21\u2010Smad3\u2010Jagged1\u2010Notch1\u2010Slug signaling in TSCC and to investigate its roles in TSCC progression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Notch1, Smad3, Jagged1 and Slug proteins and mRNA expression were detected in specimens from 89 cases of patients. We analyzed the correlation between their expressions and histological grade, clinical stage and lymph node metastasis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Notch1, Smad3, Jagged1 and Slug mRNA expressions in TSCC were higher than normal tissue (<jats:italic>P<\/jats:italic> &lt;0.05). The protein expression of Notch1 and Smad3 in TSCC were higher (\u03c7<jats:sup>2<\/jats:sup> =7.30, <jats:italic>P<\/jats:italic> &lt;0.01 and \u03c7<jats:sup>2<\/jats:sup> = 5.84, <jats:italic>P<\/jats:italic> &lt;0.05). Notch1 and Smad3 expressions were correlated with clinical stage (\u03c7<jats:sup>2<\/jats:sup> =18.81, <jats:italic>P<\/jats:italic>&lt;0.01; \u03c7<jats:sup>2<\/jats:sup> =22.29, <jats:italic>P<\/jats:italic>&lt;0.01), but not Jagged1 (\u03c7<jats:sup>2<\/jats:sup> =0.53, <jats:italic>P<\/jats:italic>&gt;0.05). The Slug protein expression was correlated with clinical stage. The positive rate of Notch1 was higher in lymph node metastases positive cases (\u03c7<jats:sup>2<\/jats:sup>=7.30, <jats:italic>P<\/jats:italic>&lt;0.01). Moreover, higher expression of Jagged1 was found in lymph node positive cases (\u03c7<jats:sup>2<\/jats:sup> =10.82, <jats:italic>P<\/jats:italic>&lt;0.01).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The key protein expression in TGF\u03b21\u2010Smad3\u2010Jagged1\u2010Notch1\u2010Slug signaling pathway significantly correlated with the clinicopathological features of TSCC patients. It's potential as a biomarker and a novel therapeutic target for TSCC patients at risk of metastasis. It may play an irreplaceable role in TSCC progression which may attribute to promoting EMT which enhances cell migration, invasion and metastasis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12406","type":"journal-article","created":{"date-parts":[[2016,1,14]],"date-time":"2016-01-14T09:00:53Z","timestamp":1452762053000},"page":"486-493","source":"Crossref","is-referenced-by-count":21,"title":["TGF\u03b21\u2010Smad3\u2010Jagged1\u2010Notch1\u2013Slug signaling pathway takes part in tumorigenesis and progress of tongue squamous cell carcinoma"],"prefix":"10.1111","volume":"45","author":[{"given":"Tonghan","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Affiliated Zhongshan Hospital Sun Yat\u2010sen University Zhongshan Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lizhong","family":"Liang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Fifth Affiliated Hospital Sun Yat\u2010sen University Zhuhai Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoling","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Medicine Intensive Care Unit Affiliated Zhongshan Hospital Sun Yat\u2010sen University Zhongshan Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji\u2010nan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Affiliated Zhongshan Hospital Sun Yat\u2010sen University Zhongshan Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jueyao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Affiliated Zhongshan Hospital Sun Yat\u2010sen University Zhongshan Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kui","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Affiliated Zhongshan Hospital Sun Yat\u2010sen University Zhongshan Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qiaoyi","family":"Zheng","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Affiliated Zhongshan Hospital Sun Yat\u2010sen University Zhongshan Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongzhang","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Guanghua School of Stomatology Hospital of Stomatology Sun Yat\u2010sen University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gui\u2010qing","family":"Liao","sequence":"additional","affiliation":[{"name":"Department of Oral and 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