{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T15:43:28Z","timestamp":1774367008095,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T00:00:00Z","timestamp":1452470400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"publisher","award":["24592766"],"award-info":[{"award-number":["24592766"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000646","name":"Japan Society for the Promotion of Science London","doi-asserted-by":"publisher","award":["26462783"],"award-info":[{"award-number":["26462783"]}],"id":[{"id":"10.13039\/501100000646","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2016,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Cell migration and invasion through interstitial tissues are dependent upon several specialized characteristics of the migratory cell notably generation of proteolytic membranous protrusions or invadopodia. Ameloblastoma is a benign odontogenic epithelial neoplasm with a locally infiltrative behaviour. Cortactin and MMT1\u2010MMP are two invadopodia proteins implicated in its local invasiveness. Other invadopodia regulators, namely N\u2010WASP, WIP and Src kinase remain unclarified. This study addresses their roles in ameloblastoma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and method<\/jats:title><jats:p>Eighty\u2010seven paraffin\u2010embedded ameloblastoma cases (20 unicystic, 47 solid\/multicystic, 3 desmoplastic and 17 recurrent) were subjected to immunohistochemistry for expression of cortactin, N\u2010WASP, WIP, Src kinase and F\u2010actin, and findings correlated with clinicopathological parameters.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Invadopodia proteins (except Src kinase) and F\u2010actin were widely detected in ameloblastoma (cortactin: <jats:italic>n<\/jats:italic> = 73\/87, 83.9%; N\u2010WASP: <jats:italic>n<\/jats:italic> = 59\/87; 67.8%; WIP: <jats:italic>n<\/jats:italic> = 77\/87; 88.5%; and F\u2010actin: <jats:italic>n<\/jats:italic> = 87\/87, 100%). Protein localization was mainly cytoplasmic and\/or membranous, and occasionally nuclear for F\u2010actin. Cortactin, which functions as an actin\u2010scaffolding protein, demonstrated significantly higher expression levels within ameloblastoma tumoral epithelium than in stroma (<jats:italic>P<\/jats:italic> &lt; 0.05). N\u2010WASP, which coordinates actin polymerization and invadopodia\u2010mediated extracellular matrix degradation, was overexpressed in the solid\/multicystic subtype (<jats:italic>P<\/jats:italic> &lt; 0.05). WIP, an upstream regulator of N\u2010WASP, and F\u2010actin were significantly upregulated along the tumour invasive front compared to tumour centres (<jats:italic>P<\/jats:italic> &lt; 0.05). Except for males with cortactin overexpression, other clinical parameters (age, ethnicity and anatomical site) showed no significant correlations.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Present results suggest that local invasiveness of ameloblastoma is dependent upon the migratory potential of its tumour cells as defined by their distribution of cortactin, N\u2010WASP and WIP in correlation with F\u2010actin cytoskeletal dynamics.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12417","type":"journal-article","created":{"date-parts":[[2016,1,11]],"date-time":"2016-01-11T05:48:13Z","timestamp":1452491293000},"page":"591-598","source":"Crossref","is-referenced-by-count":12,"title":["Invadopodia proteins, cortactin, N\u2010WASP and WIP differentially promote local invasiveness in ameloblastoma"],"prefix":"10.1111","volume":"45","author":[{"given":"Chong Huat","family":"Siar","sequence":"first","affiliation":[{"name":"Department of Oro\u2010Maxillofacial Surgical and Medical Sciences Faculty of Dentistry University of Malaya Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zainal Ariff Bin Abdul","family":"Rahman","sequence":"additional","affiliation":[{"name":"Department of Oro\u2010Maxillofacial Surgical and Medical Sciences Faculty of Dentistry University of Malaya Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hidetsugu","family":"Tsujigiwa","sequence":"additional","affiliation":[{"name":"Laboratory of Histopathology Department of Life Science Faculty of Science Okayama University of Science Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kamila","family":"Mohamed Om Alblazi","sequence":"additional","affiliation":[{"name":"Department of Oro\u2010Maxillofacial Surgical and Medical Sciences Faculty of Dentistry University of Malaya Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hitoshi","family":"Nagatsuka","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Medicine Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama University Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kok Han","family":"Ng","sequence":"additional","affiliation":[{"name":"Unit of Stomatology Cancer Research Centre Institute for Medical Research Kuala Lumpur Malaysia"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,1,11]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2006.07.001"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.093799"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2014.07.003"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3141"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20296"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-05-2177"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.008037"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2004.12.055"},{"key":"e_1_2_5_10_1","first-page":"263","article-title":"Roles of cortactin in tumor pathogenesis","volume":"1775","author":"Buday L","year":"2007","journal-title":"Biochim Biophys Acta"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-014-2976-8"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-2050.2007.00775.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.389"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2010.05.030"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12253-009-9245-y"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1002\/cm.20361"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2009-10-6-226"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2012.12.005"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2012.06.002"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2005.08.004"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00017-14"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-09-4149"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.092726"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10585-007-9120-8"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2014.07.008"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2012.06.003"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-10-1432"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2012.02.004"},{"key":"e_1_2_5_29_1","first-page":"296","volume-title":"World Health Organization classification of tumours. Pathology and genetics of head and neck tumours","author":"Gardner DG","year":"2005"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2014.09.001"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2559.2011.03995.x"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tripleo.2008.11.021"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-015-1914-2"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)01035-7"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2010.01654.x"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc822"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1658"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2011.05.008"}],"container-title":["Journal of Oral Pathology &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12417","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12417","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.12417","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,23]],"date-time":"2023-09-23T18:33:52Z","timestamp":1695494032000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jop.12417"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,1,11]]},"references-count":37,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2016,9]]}},"alternative-id":["10.1111\/jop.12417"],"URL":"https:\/\/doi.org\/10.1111\/jop.12417","archive":["Portico"],"relation":{},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,1,11]]}}}