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This study aimed to explore the regulation of disk angiogenesis in the perforated disks.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Expressions of vascular endothelial growth factor (<jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>), angiogenin\u20101 (Ang\u20101), chondromodulin\u20101 (ChM\u20101), and thrombospondins\u20101 (<jats:styled-content style=\"fixed-case\">TSP<\/jats:styled-content>\u20101) were compared between healthy and perforated <jats:styled-content style=\"fixed-case\">TMJ<\/jats:styled-content> disk cells with or without interleukin\u20101\u03b2 (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2) incubation. The tube formation, cell migration, and expressions of matrix\u2010metalloproteinases (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>s) in human umbilical vein endothelial cell line (<jats:styled-content style=\"fixed-case\">HUV<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">EC<\/jats:styled-content>\u2010C) were investigated in conditional media of disk cells. Western blot was performed to determine protein level of <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content>, Ang\u20101, ChM\u20101 and <jats:styled-content style=\"fixed-case\">TSP<\/jats:styled-content>\u20101 in <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2\u2010induced disk cells cultured by <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB\u2010 or P38\u2010specific pathway inhibitors, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Conditional media from perforated disk cells induced more tube formation, cell migration, and <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>s\u2019 expression in <jats:styled-content style=\"fixed-case\">HUV<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">EC<\/jats:styled-content>\u2010C. Expressions of <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> and Ang\u20101 were significantly higher, and ChM\u20101 and <jats:styled-content style=\"fixed-case\">TSP<\/jats:styled-content>\u20101 were lower in perforated disks compared to healthy disks. The <jats:styled-content style=\"fixed-case\">VEGFA<\/jats:styled-content> concentration was 291.1 \u00b1 36.09 pg\/ml in perforated disk cell conditioned media, markedly larger than that in <jats:styled-content style=\"fixed-case\">NDCCM<\/jats:styled-content> (144.9 \u00b1 33.69 pg\/ml). <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 induced <jats:styled-content style=\"fixed-case\">VEGF<\/jats:styled-content> through <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB signaling pathway and Ang\u20101 through p38 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> pathway, while repressed expression of ChM\u20101 and <jats:styled-content style=\"fixed-case\">TSP<\/jats:styled-content>\u20101 was through <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB pathway. Blockade of each pathway markedly restrained inducing effect of cultural media on <jats:styled-content style=\"fixed-case\">HUV<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">EC<\/jats:styled-content>\u2010C tube formation and migration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Perforated disk cells secreted more angiogenic factors which might induced via <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12420","type":"journal-article","created":{"date-parts":[[2016,1,18]],"date-time":"2016-01-18T04:56:46Z","timestamp":1453093006000},"page":"605-612","source":"Crossref","is-referenced-by-count":13,"title":["<scp>IL<\/scp>\u20101\u03b2\u2010regulating angiogenic factors expression in perforated temporomandibular disk cells via <scp>NF<\/scp>\u2010\u03baB pathway"],"prefix":"10.1111","volume":"45","author":[{"given":"Jie","family":"Xu","sequence":"first","affiliation":[{"name":"State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM) School and Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hengxing","family":"Cai","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM) School and Hospital of Stomatology Wuhan University Wuhan China"},{"name":"Department of Oral and Maxillofacial Surgery School and Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qinggong","family":"Meng","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM) School and Hospital of Stomatology Wuhan University Wuhan China"},{"name":"Department of Oral and Maxillofacial Surgery School and Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yingjie","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM) School and Hospital of Stomatology Wuhan University Wuhan China"},{"name":"Department of Oral and Maxillofacial Surgery School and Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Guoxin","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM) School and Hospital of Stomatology Wuhan University Wuhan China"},{"name":"Department of Oral and Maxillofacial Surgery School and Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wei","family":"Fang","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM) School and Hospital of Stomatology Wuhan University Wuhan China"},{"name":"Department of Oral and Maxillofacial Surgery School and Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xing","family":"Long","sequence":"additional","affiliation":[{"name":"State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM) School and Hospital of Stomatology Wuhan University Wuhan China"},{"name":"Department of Oral and Maxillofacial Surgery School and Hospital of Stomatology Wuhan University Wuhan China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,1,17]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0003-9969(02)00207-8"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2009.00831.x"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a006627"},{"key":"e_1_2_5_5_1","first-page":"293","article-title":"Chondrocyte\u2010like apoptosis in temporomandibular joint disc internal derangement as a repair\u2010limiting mechanism. 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