{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,19]],"date-time":"2026-03-19T12:23:29Z","timestamp":1773923009058,"version":"3.50.1"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,4,15]],"date-time":"2016-04-15T00:00:00Z","timestamp":1460678400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2016,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Unicystic ameloblastoma, an odontogenic neoplasm, presents clinical and radiographic similarities with dentigerous and radicular cysts, non\u2010neoplastic lesions. It is not always possible to reach a final diagnosis with the incisional biopsy, leading to inappropriate treatment. The <jats:styled-content style=\"fixed-case\">BRAFV<\/jats:styled-content>600E activating mutation has been reported in a high proportion of ameloblastomas. The purpose of the study was to assess the utility of the detection of the <jats:styled-content style=\"fixed-case\">BRAFV<\/jats:styled-content>600E mutation in the differential diagnosis of unicystic ameloblastoma with dentigerous and radicular cysts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010six archival samples were included, comprising eight unicystic ameloblastomas (UAs), nine dentigerous and nine radicular cysts. The mutation was assessed in all samples by anti\u2010<jats:styled-content style=\"fixed-case\">BRAFV<\/jats:styled-content>600E (clone <jats:styled-content style=\"fixed-case\">VE<\/jats:styled-content>1) immunohistochemistry (<jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content>) and by TaqMan mutation detection <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> assay. Sanger sequencing was further carried out when samples showed conflicting results in the <jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Although all UAs (8\/8) showed positive uniform <jats:styled-content style=\"fixed-case\">BRAFV<\/jats:styled-content>600E staining along the epithelial lining length, the mutation was not confirmed by <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> and Sanger sequencing in three samples. Positive staining for the <jats:styled-content style=\"fixed-case\">BRAFV<\/jats:styled-content>600E protein was observed in one dentigerous cyst, but it was not confirmed by the molecular methods. Furthermore, 2\/9 dentigerous cysts and 2\/9 radicular cysts showed non\u2010specific immunostaining of the epithelium or plasma cells. None of the dentigerous or radicular cysts cases presented the <jats:styled-content style=\"fixed-case\">BRAFV<\/jats:styled-content>600E mutation in the <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">BRAFV<\/jats:styled-content>600E antibody (clone <jats:styled-content style=\"fixed-case\">VE<\/jats:styled-content>1) <jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content> may show non\u2010specific staining, but molecular assays may be useful for the diagnosis of unicystic ameloblastoma, in conjunction with clinical, radiological and histopathological features.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12443","type":"journal-article","created":{"date-parts":[[2016,4,16]],"date-time":"2016-04-16T01:56:08Z","timestamp":1460771768000},"page":"780-785","source":"Crossref","is-referenced-by-count":57,"title":["<scp>BRAFV<\/scp>600E mutation in the diagnosis of unicystic ameloblastoma"],"prefix":"10.1111","volume":"45","author":[{"given":"N\u00fabia Braga","family":"Pereira","sequence":"first","affiliation":[{"name":"Department of Oral Surgery and Pathology School of Dentistry Universidade Federal de Minas Gerais\u2013UFMG Belo Horizonte Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Karuza Maria Alves","family":"Pereira","sequence":"additional","affiliation":[{"name":"School of Dentistry Universidade Federal do Cear\u00e1\u2013UFC Cear\u00e1 Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruna Pizziolo","family":"Coura","sequence":"additional","affiliation":[{"name":"Department of Oral 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