{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T13:36:21Z","timestamp":1791207381167,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,8,26]],"date-time":"2016-08-26T00:00:00Z","timestamp":1472169600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003399","name":"Science and Technology Commission of Shanghai Municipality","doi-asserted-by":"publisher","award":["15411950300"],"award-info":[{"award-number":["15411950300"]}],"id":[{"id":"10.13039\/501100003399","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2016,11]]},"abstract":"Background<\/jats:title>Oral squamous cell carcinoma (OSCC<\/jats:styled-content>) accounts for 95% of all oral cancer with higher mortality and morbidity rates worldwide. However, the potential molecular mechanism of OSCC<\/jats:styled-content> remains largely unclear. Myosin VI<\/jats:styled-content> (MYO<\/jats:styled-content>6<\/jats:italic>) is a unique actin motor and reported to be overexpressed in several cancers. This study aims to examine the functional relationship between OSCC<\/jats:styled-content> and MYO<\/jats:styled-content>6<\/jats:italic>.<\/jats:p><\/jats:sec>Methods<\/jats:title>The mRNA<\/jats:styled-content> expression of MYO<\/jats:styled-content>6<\/jats:italic> was firstly investigated by analyzing data derived from Oncomine database. On the basis of the results, the expression of MYO<\/jats:styled-content>6<\/jats:italic> was knocked down using lentivirus\u2010delivered RNA<\/jats:styled-content> interference in human OSCC<\/jats:styled-content> cell line CAL<\/jats:styled-content>27, as confirmed by qPCR<\/jats:styled-content> and Western blot analysis. Stable MYO<\/jats:styled-content>6<\/jats:italic> knockdown cells were employed to determine the effects of MYO<\/jats:styled-content>6<\/jats:italic>\u2010silencing on cell growth by MTT<\/jats:styled-content>, colony formation and cell cycle distribution and apoptosis by flow cytometry assay. Moreover, the expressions of cell apoptotic proteins were examined by Western blot analysis.<\/jats:p><\/jats:sec>Results<\/jats:title>We first observed MYO<\/jats:styled-content>6<\/jats:italic> was overexpressed in tongue squamous cell carcinoma TSCC<\/jats:styled-content> belongs to OSCC<\/jats:styled-content>, compared with normal tissues. For cellular analysis, shRNA<\/jats:styled-content> sequences against MYO<\/jats:styled-content>6<\/jats:italic> could efficiently reduce its expression in CAL<\/jats:styled-content>27 cells. Knockdown of MYO<\/jats:styled-content>6<\/jats:italic> significantly decreased cell proliferation, caused cell cycle arrest at G2\/M phase, and promoted cell apoptosis. Moreover, cell apoptosis\u2010associated proteins, caspase\u20103 and PARP<\/jats:styled-content>, were obviously upregulated in CAL<\/jats:styled-content>27 after MYO<\/jats:styled-content>6<\/jats:italic>\u2010silencing.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>MYO<\/jats:styled-content>6<\/jats:italic> could play an essential role in the growth of OSCC<\/jats:styled-content> cells via regulation of cell cycle progression and apoptosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12448","type":"journal-article","created":{"date-parts":[[2016,8,26]],"date-time":"2016-08-26T07:42:59Z","timestamp":1472197379000},"page":"740-745","source":"Crossref","is-referenced-by-count":18,"title":["Knockdown of Myosin 6<\/i> inhibits proliferation of oral squamous cell carcinoma cells"],"prefix":"10.1111","volume":"45","author":[{"given":"Xinyu","family":"Zhang","sequence":"first","affiliation":[{"name":"Department of Oral\u2010maxillofacial Head and Neck Surgery Ninth People's Hospital College of Stomatology Shanghai Key Laboratory of Stomatology Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiquan","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Oral\u2010maxillofacial Head and Neck Surgery Sun Yat\u2010sen Memorial Hospital Guang Zhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yongjie","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Oral\u2010maxillofacial Head and Neck Surgery Ninth People's Hospital College of Stomatology Shanghai Key Laboratory of Stomatology Shanghai Jiao Tong University School of Medicine Shanghai China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liu","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Oral\u2010maxillofacial Head and Neck Surgery Ninth People's Hospital College of Stomatology Shanghai Key Laboratory of Stomatology 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