{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,6]],"date-time":"2026-05-06T05:36:08Z","timestamp":1778045768357,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2016,5,9]],"date-time":"2016-05-09T00:00:00Z","timestamp":1462752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["478261\/2011\u20100"],"award-info":[{"award-number":["478261\/2011\u20100"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2016,11]]},"abstract":"Background<\/jats:title>Epigenetic modifications, including DNA<\/jats:styled-content> methylation of tumor suppressor genes carried out by DNA<\/jats:styled-content> methyltransferases (DNMT<\/jats:styled-content>s), are important events in carcinogenesis. Although there are studies concerning to its expression in several cancer types, DNMTs<\/jats:styled-content> expression pattern is not known in photoinduced lip carcinogenesis. The aim of this study was to investigate the immunoexpression of DNMT<\/jats:styled-content>s 1, 3a, and 3b in lip precancerous lesion (actinic cheilitis) and cancer.<\/jats:p><\/jats:sec>Methods<\/jats:title>Thirty cases of actinic cheilitis (AC<\/jats:styled-content>), thirty cases of lip squamous cell carcinoma (LSCC<\/jats:styled-content>), and twenty cases of non\u2010neoplastic tissue (NNT<\/jats:styled-content>) were selected for immunohistochemical investigation of DNMT<\/jats:styled-content>s 1, 3a, and 3b.<\/jats:p><\/jats:sec>Results<\/jats:title>Nuclear DNMT<\/jats:styled-content> 1 immunoreactivity was significantly higher in the LSCC<\/jats:styled-content> group (68.6%) compared with NNT<\/jats:styled-content> (47%), and nuclear DNMT<\/jats:styled-content> 3b was higher in LSCC<\/jats:styled-content> (70.9%) than in NNT<\/jats:styled-content> (37.9%) and in AC<\/jats:styled-content> (44%). Only DNMT<\/jats:styled-content> 3a showed both higher nuclear and cytoplasmic expression in AC<\/jats:styled-content> (35.9% and 35.5%, respectively) than in NNT<\/jats:styled-content> (4.4% and 16.1%, respectively) and LSCC<\/jats:styled-content> (8.8% and 13.2%, respectively) (P<\/jats:italic> < 0.05).<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The results suggested that DNMT<\/jats:styled-content> 3a could play a key role in the methylation process of initial steps of UV<\/jats:styled-content> carcinogenesis present in AC<\/jats:styled-content> while DNMT<\/jats:styled-content> 3b could be responsible for de novo<\/jats:italic> methylation in already established lip cancer.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12453","type":"journal-article","created":{"date-parts":[[2016,5,9]],"date-time":"2016-05-09T15:12:08Z","timestamp":1462806728000},"page":"774-779","source":"Crossref","is-referenced-by-count":15,"title":["Immunohistochemical expression of DNA<\/scp> methyltransferases 1, 3a, and 3b in actinic cheilitis and lip squamous cell carcinomas"],"prefix":"10.1111","volume":"45","author":[{"given":"Filipe I.","family":"Daniel","sequence":"first","affiliation":[{"name":"Pathology Department and Dentistry Graduate Program Federal University of Santa Catarina Florianopolis Santa Catarina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Soraia R.","family":"Alves","sequence":"additional","affiliation":[{"name":"Private Dentist Florianopolis Santa Catarina Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniella S. 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