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One of the most remarkable malignancies of the head and neck is the cancer of the nasopharynx, with a strong metastatic tendency already in the early stage. Besides the conventional pathways of metastasis formation, the information content of exosomes produced by the cancer cells may play a key role in metastatic transformation. The aim of this study was to investigate how stressors alter the characteristic of tumor derived exosomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In our experimental model, we compared the quantity and content of exosomes produced by a nasopharyngeal carcinoma cell line (5\u20108F) under conventional (chemotherapy) and alternative (Ag\u2013TiO<jats:sub>2<\/jats:sub>\u2010catalyzed reactive oxygen species generation) cytostatic treatment. After isolation, exosomes were identified by atomic force microscopy and quantified with Nanosight <jats:styled-content style=\"fixed-case\">NS<\/jats:styled-content>500 device. Micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> content of them was analyzed using <jats:styled-content style=\"fixed-case\">SOL<\/jats:styled-content>iD 5500xl technology. The sequences were annotated in <jats:styled-content style=\"fixed-case\">CLC<\/jats:styled-content> Genomics Workbench version 5.5.1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Beyond the classic chemotherapeutic agent (doxorubicin), Ag\u2013TiO<jats:sub>2<\/jats:sub> in a photo\u2010catalytic process also showed cytostatic activity. Tumor cell damage induced by the cytostatic treatments significantly altered the number of released exosomes and led to the predominance of tumor suppressors in the exosomal mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> profile.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our results suggest that the intercellular communication between tumor cells and surrounding stroma cells can be altered by microenvironment which increased quantity of exosomes and diversity of mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in this study. Imbalance of oncogenic and tumor suppressor mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s caused by cytostatic treatments may influence the antiproliferative and metastasis inhibitory effect of cytostatic agents.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12486","type":"journal-article","created":{"date-parts":[[2016,9,6]],"date-time":"2016-09-06T14:17:01Z","timestamp":1473171421000},"page":"259-266","source":"Crossref","is-referenced-by-count":46,"title":["Stressors alter intercellular communication and exosome profile of nasopharyngeal carcinoma cells"],"prefix":"10.1111","volume":"46","author":[{"given":"Maria","family":"Harmati","sequence":"first","affiliation":[{"name":"Laboratory of Microscopic Image Analysis and Machine Learning Institute of Biochemistry Biological Research Centre of the Hungarian Academy of Sciences Szeged 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