{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T12:55:27Z","timestamp":1765976127225},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2016,9,8]],"date-time":"2016-09-08T00:00:00Z","timestamp":1473292800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2017,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Nicotine hinders the regenerative potentials of human periodontal ligament\u2010derived stem cells (<jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s) and delays the healing process of periodontal diseases, but the underlying mechanism remains unclear. miR\u20101305 upregulation and its potential target <jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2 downregulation exist in the <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s exposed to nicotine. In this study, we aimed to investigate whether nicotine inhibits <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content> proliferation, migration, and osteogenic differentiation by increasing miR\u20101305 level and decreasing <jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2 level.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) and Western blot assays were performed to detect the expression levels of miR\u20101305 and <jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2 in the <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s exposed to nicotine, respectively. <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s with miR\u20101305 overexpression, low expression, or <jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2 overexpression were constructed by lipofectin transfection. <jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content>, migration, and Western blot assays were applied to assess the effect of miR\u20101305 on <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content> proliferation, migration, and osteogenic differentiation, respectively. Target prediction and luciferase reporter assays were performed to investigate the targets of miR\u20101305.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Nicotine promoted miR\u20101305 expression and inhibited <jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2 expression in <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s. Cell proliferation, migration, and differentiation detection showed that nicotine suppressed proliferation, migration, and osteogenic differentiation of <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s, and restoration of miR\u20101305 relieved the inhibitory effect of nicotine on <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s. Moreover, we identified and validated that <jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2 was a direct target of miR\u20101305, and upregulation of <jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2 had similar effects with the downregulation of miR\u20101305 on relieving the inhibitory effect of nicotine on <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Nicotine suppresses proliferation, migration, and osteogenic differentiation of <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s, and restoration of miR\u20101305 relieves the inhibitory effect of nicotine on <jats:styled-content style=\"fixed-case\">PDLSC<\/jats:styled-content>s depending on its target <jats:styled-content style=\"fixed-case\">RUNX<\/jats:styled-content>2.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12492","type":"journal-article","created":{"date-parts":[[2016,9,8]],"date-time":"2016-09-08T10:57:21Z","timestamp":1473332241000},"page":"313-320","source":"Crossref","is-referenced-by-count":24,"title":["Restoration of miR\u20101305 relieves the inhibitory effect of nicotine on periodontal ligament\u2010derived stem cell proliferation, migration, and osteogenic differentiation"],"prefix":"10.1111","volume":"46","author":[{"given":"Zhuo","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Stomatology Zhengzhou Central Hospital Affiliated to Zhengzhou University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hui\u2010Li","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Stomatology Zhengzhou Central Hospital Affiliated to Zhengzhou University Zhengzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2016,9,8]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcpe.12368"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-4-431-54070-0_25"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)16627-0"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-051X.2011.01716.x"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2006.00870.x"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1634\/stemcells.2007-0734"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2012.0434"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10059-012-2172-x"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0083102"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.02.003"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature02871"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1080372"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep07828"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0000079"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2280"},{"key":"e_1_2_5_18_1","first-page":"1513","article-title":"Quitting smoking among adults\u2013United States, 2001\u20132010","volume":"60","author":"Centers for Disease Control and Prevention (CDC)","year":"2011","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMc1102459"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.injury.2008.12.011"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1177\/153537020222700206"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.jfas.2009.04.008"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1001\/archsurg.2012.5"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0809890"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00264-011-1243-5"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0765.2006.00915.x"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00784-007-0170-8"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.reprotox.2010.10.007"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2011.09.005"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1089\/ten.tea.2011.0545"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-012-1393-9"}],"container-title":["Journal of Oral Pathology &amp; 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