{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T14:16:35Z","timestamp":1768313795011,"version":"3.49.0"},"reference-count":28,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,10,21]],"date-time":"2016-10-21T00:00:00Z","timestamp":1477008000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100008233","name":"Shanghai Jiao Tong University School of Medicine","doi-asserted-by":"publisher","award":["14XJ10010"],"award-info":[{"award-number":["14XJ10010"]}],"id":[{"id":"10.13039\/501100008233","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate microarchitectural changes in condylar cartilage and associated subchondral bone after bisphosphonates treatment using an ovariectomized (<jats:styled-content style=\"fixed-case\">OVX<\/jats:styled-content>) osteoporosis rat model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty six\u2010month\u2010old female Sprague\u2010Dawley rats were randomly divided into sham, <jats:styled-content style=\"fixed-case\">OVX<\/jats:styled-content>, and risedronate (<jats:styled-content style=\"fixed-case\">RIS<\/jats:styled-content>)\u2010treated groups. Both <jats:styled-content style=\"fixed-case\">OVX<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">RIS<\/jats:styled-content> groups received bilateral ovariectomy. <jats:styled-content style=\"fixed-case\">OVX<\/jats:styled-content> group was treated subcutaneously with saline, whereas <jats:styled-content style=\"fixed-case\">RIS<\/jats:styled-content> group received risedronate treatment (2.4 \u03bcg\/kg) subcutaneously for 3 months. At the end of 3 months, animals were sacrificed and the entire condyles were harvested for micro\u2010<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content> and histological analyses. Immunohistochemistry (<jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content>) was performed to assess the expression of type I\/<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> collagen protein by semiquantitative imaging analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Micro\u2010<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content> analysis showed <jats:styled-content style=\"fixed-case\">OVX<\/jats:styled-content> group had significant condylar subchondral bone loss compared to sham as shown by significant decrease in bone volume fraction (<jats:italic>P<\/jats:italic> = 0.028), trabecular thickness (<jats:italic>P<\/jats:italic> = 0.041), and significant increase in trabecular spacing (<jats:italic>P<\/jats:italic> = 0.003). In <jats:styled-content style=\"fixed-case\">RIS<\/jats:styled-content> group, partial inhibition of <jats:styled-content style=\"fixed-case\">OVX<\/jats:styled-content>\u2010induced bone loss was detected. <jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content> staining showed proliferative layer of condylar cartilage reduced, while hypertrophic chondrocyte layer increased significantly in <jats:styled-content style=\"fixed-case\">RIS<\/jats:styled-content> group compared to sham and <jats:styled-content style=\"fixed-case\">OVX<\/jats:styled-content> groups. <jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content> showed reduced expression of Col I in both the <jats:styled-content style=\"fixed-case\">OVX<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">RIS<\/jats:styled-content> groups, whereas expression of Col <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> was reduced in the <jats:styled-content style=\"fixed-case\">OVX<\/jats:styled-content> group but increased in the <jats:styled-content style=\"fixed-case\">RIS<\/jats:styled-content> group.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings suggest that systemic bisphosphonate treatment influences the structure and ossification of condylar cartilage and it has a dual action on condyle in a postmenopausal osteoporosis rat model which raises the concerns for the potential side effects of <jats:styled-content style=\"fixed-case\">BP<\/jats:styled-content>s on condyle to elder patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12499","type":"journal-article","created":{"date-parts":[[2016,10,21]],"date-time":"2016-10-21T06:23:15Z","timestamp":1477030995000},"page":"398-404","source":"Crossref","is-referenced-by-count":16,"title":["Effects of bisphosphonates on mandibular condyle of ovariectomized osteoporotic rats using micro\u2010ct and histomorphometric 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