{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,27]],"date-time":"2026-05-27T16:44:16Z","timestamp":1779900256468,"version":"3.53.1"},"reference-count":29,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2016,10,15]],"date-time":"2016-10-15T00:00:00Z","timestamp":1476489600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100006345","name":"Uniwersytet Mikolaja Kopernika w Toruniu","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100006345","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2017,5]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The role of <jats:italic><jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content><\/jats:italic> mutations in cancerogenesis has been demonstrated in several solid tumor types. However, in salivary gland tumors, this genetic alteration is very uncommon, and its role still remains unclear. Thus, the aim of this study was to analyze <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> V600E (<jats:styled-content style=\"fixed-case\">VE<\/jats:styled-content>1) protein expression with <jats:italic><jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content><\/jats:italic> mutation status in codon 600, in malignant and benign salivary gland tumors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Studies were performed on archived formalin\u2010fixed paraffin\u2010embedded tissue sections derived from 95 patients who underwent surgery for tumors of the salivary gland. Immunohistochemical staining (<jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content>) on tissue microarray slides was performed for evaluation of <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> V600E (<jats:styled-content style=\"fixed-case\">VE<\/jats:styled-content>1) protein expression, and the automatic molecular diagnostics platform was used for the evaluation of mutations in codon 600 of <jats:italic><jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content><\/jats:italic> gene.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content> cytoplasmic expression of <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> V600E (<jats:styled-content style=\"fixed-case\">VE<\/jats:styled-content>1) protein was found in two of 95 cases: one case of adenocarcinoma <jats:styled-content style=\"fixed-case\">NOS<\/jats:styled-content> (one of three; 33%) and one case of carcinoma ex pleomorphic adenoma (one of five; 20%). Although, in <jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content> studies, nuclear <jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> V600E (<jats:styled-content style=\"fixed-case\">VE<\/jats:styled-content>1) protein expression was found in 14 (15%) of the analyzed cases: nine of 28 (32%) cases of pleomorphic adenoma, three of five (60%) cases of ductal carcinoma, one of nine (11%) case of mucoepidermoid carcinoma, and in one of five (20%) case of carcinoma ex pleomorphic adenoma. All cases were negative for polymerase chain reaction <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010based analyses of <jats:italic><jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content><\/jats:italic> mutations in codon 600.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In studied salivary gland cancers, no <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>\u2010based prove mutations of <jats:italic><jats:styled-content style=\"fixed-case\">BRAF<\/jats:styled-content> V600<\/jats:italic> were detected. Further molecular analyses are necessary to rapid molecular arrays for the identification of specific mutations, optimal for individualized targeted therapies.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12504","type":"journal-article","created":{"date-parts":[[2016,9,28]],"date-time":"2016-09-28T14:15:31Z","timestamp":1475072131000},"page":"340-345","source":"Crossref","is-referenced-by-count":9,"title":["Assessment of <scp>BRAF<\/scp> V600E (<scp>VE<\/scp>1) protein expression and <i>BRAF<\/i> gene mutation status in codon 600 in benign and malignant salivary gland neoplasms"],"prefix":"10.1111","volume":"46","author":[{"given":"Magdalena","family":"Bodnar","sequence":"first","affiliation":[{"name":"Department of Clinical Pathomorphology Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Torun Bydgoszcz Poland"},{"name":"Department of Otolaryngology and Laryngeal Oncology K. Marcinkowski University of Medical Sciences Poznan Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pawe\u0142","family":"Burduk","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology and Laryngological Oncology Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Torun Bydgoszcz Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Antosik","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathomorphology Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Torun Bydgoszcz Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ma\u0142gorzata","family":"Jarmuz\u2010Szymczak","sequence":"additional","affiliation":[{"name":"Institute of Human Genetics Department of Cancer Genetics Polish Academy of Sciences Poznan Poland"},{"name":"Department of Hematology and Bone Marrow Transplantation K. Marcinkowski University of Medical Sciences Poznan Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Malgorzata","family":"Wierzbicka","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology and Laryngeal Oncology K. Marcinkowski University of Medical Sciences Poznan Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrzej","family":"Marszalek","sequence":"additional","affiliation":[{"name":"Department of Clinical Pathomorphology Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Torun Bydgoszcz Poland"},{"name":"Oncologic Pathology and Prophylaxis Poznan University of Medical Sciences &amp; Greater Poland Cancer Center Poznan Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,10,15]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21254"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2006.12.012"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2015.12.015"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1517\/14728222.2011.645805"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.07.092"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAT.0b013e328360b61d"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAT.0000000000000119"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.5430\/jst.v5n1p44"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12443"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2015.09.004"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.24332"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.4015"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12105-013-0462-z"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.leu.2402945"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature08314"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm3979"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/2193\u20101801\u20102\u2010679"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-12-1842"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1593\/neo.10356"},{"key":"e_1_2_5_21_1","first-page":"3","article-title":"Three cases of rare salivary gland tumours: a molecular study of TP53, CDKN2A\/ARF, RAS, BRAF, PTEN, MAPK2 and EGFR genes","volume":"26","author":"Falbo V","year":"2011","journal-title":"Oncol Rep"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/mdt338"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1155\/2009\/501736"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.22238"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.semdp.2015.02.010"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.humpath.2012.07.010"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00401-011-0887-y"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1097\/PAS.0b013e318246b527"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1111\/his.12992"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.2013.126"}],"container-title":["Journal of Oral Pathology &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12504","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12504","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.12504","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T19:05:46Z","timestamp":1696187146000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jop.12504"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,10,15]]},"references-count":29,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2017,5]]}},"alternative-id":["10.1111\/jop.12504"],"URL":"https:\/\/doi.org\/10.1111\/jop.12504","archive":["Portico"],"relation":{},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,10,15]]}}}