{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:14:03Z","timestamp":1787015643037,"version":"build-2736575974"},"reference-count":29,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2017,1,16]],"date-time":"2017-01-16T00:00:00Z","timestamp":1484524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100007774","name":"Instituto de Ciencia y Tecnolog\u00eda del Distrito Federal","doi-asserted-by":"publisher","award":["286\u201011"],"award-info":[{"award-number":["286\u201011"]}],"id":[{"id":"10.13039\/100007774","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003141","name":"Consejo Nacional de Ciencia y Tecnolog\u00eda","doi-asserted-by":"publisher","award":["230110"],"award-info":[{"award-number":["230110"]}],"id":[{"id":"10.13039\/501100003141","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2017,9]]},"abstract":"<jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      Dentigerous cyst (DC) occurs in approximately 20% of jaw cysts, being the second major common odontogenic cyst, after radicular cyst. This oral lesion has the ability to destroy maxillary bones and could be the origin of several odontogenic tumors. However, molecules implicated in its pathogenesis as well as those involved in its neoplastic transformation remain unknown. Here, we established a cell population derived from a DC as an\n                      <jats:italic>in vitro<\/jats:italic>\n                      model for the study of this oral lesion.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Cell culture was performed from a DC from a 44\u2010year\u2010old male. Cells were cultured at 37\u00b0C in\n                      <jats:styled-content style=\"fixed-case\">DMEM<\/jats:styled-content>\n                      \/F12 medium containing 10% fetal bovine serum. Expression of epithelial markers was analyzed by Western blot and immunofluorescence. Ultrastructural characterization was carried out by transmission electron microscopy. Conditioned media were obtained and characterized by zymography and Western blot.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Cells showed spindle\u2010shaped morphology, but they express epithelial markers, such as cytokeratins and the odontogenic ameloblast\u2010associated protein. The ultrastructural analysis showed well\u2010formed desmosomes present in adhering contiguous cells, confirming the epithelial lineage of this cell population. Cells also contain several vesicles adjacent to plasma membrane, suggesting an active secretion. Indeed, the analysis of the conditioned medium revealed the presence of several secreted proteins, among them the matrix metalloproteinase\u20102.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>Our work provides a useful model to identify the molecular mechanisms involved in the pathogenesis of DC.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/jop.12528","type":"journal-article","created":{"date-parts":[[2016,11,24]],"date-time":"2016-11-24T02:17:04Z","timestamp":1479953824000},"page":"603-610","source":"Crossref","is-referenced-by-count":3,"title":["Establishment and characterization of a cell population derived from a dentigerous cyst"],"prefix":"10.1111","volume":"46","author":[{"given":"Marcos A.","family":"Mu\u00f1iz\u2010Lino","sequence":"first","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Rodr\u00edguez\u2010V\u00e1zquez","sequence":"additional","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bibiana","family":"Ch\u00e1vez\u2010Mungu\u00eda","sequence":"additional","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josu\u00e9 Z.","family":"Ortiz\u2010Garc\u00eda","sequence":"additional","affiliation":[{"name":"Secci\u00f3n de Posgrado e Investigaci\u00f3n Escuela Superior de Medicina del Instituto Polit\u00e9cnico Nacional Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorena","family":"Gonz\u00e1lez\u2010L\u00f3pez","sequence":"additional","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fidel C.","family":"Hern\u00e1ndez\u2010Hern\u00e1ndez","sequence":"additional","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlos","family":"Lic\u00e9aga\u2010Escalera","sequence":"additional","affiliation":[{"name":"Departamento de Cirug\u00eda Maxilofacial Hospital Ju\u00e1rez de M\u00e9xico Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro","family":"Garc\u00eda\u2010Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Odontolog\u00eda ALMARAZ\/UBIMED FES Iztacala UNAM State of Mexico Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mario A.","family":"Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Departamento de Infect\u00f3mica y Patog\u00e9nesis Molecular Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional Mexico City Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,16]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/modpathol.3880527"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/jicd.12044"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2006.00455.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijom.2010.04.048"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-662-02858-2"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bdj.4812082"},{"key":"e_1_2_5_8_1","first-page":"ZD23","article-title":"Ameloblastoma arising from a dentigerous cyst\u2010a case report","volume":"8","author":"Bhushan NS","year":"2014","journal-title":"J Clin Diagn Res"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pedex.2007.08.003"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1067\/mhn.2002.129039"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjoms.2010.11.016"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-007-9109-9"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0714.2000.290305.x"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1998.tb01943.x"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12125"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-427150-0.50060-7"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.2119\/2008-00010.Kestler"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986710793213724"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.367680"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200603161"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1080\/01913120600689806"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-15-0293"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-014-1989-3"},{"key":"e_1_2_5_24_1","first-page":"59","article-title":"Expression of odontogenic ameloblast\u2010associated and amelotin proteins in the junctional epithelium","volume":"2013","author":"Nishio C","year":"2000","journal-title":"Periodontology"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1080\/13506120802005965"},{"key":"e_1_2_5_26_1","first-page":"219","article-title":"Scanning electron microscopy of odontogenic cyst epithelium","volume":"47","author":"Philipsen HP","year":"1992","journal-title":"J Dent Assoc S Afr"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/0030-4220(81)90313-3"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/1097-0142(197210)30:4<1036::AID-CNCR2820300426>3.0.CO;2-0"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1677\/erc.1.01226"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2009.00771.x"}],"container-title":["Journal of Oral Pathology &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12528","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.12528","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T06:21:33Z","timestamp":1695536493000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jop.12528"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,1,16]]},"references-count":29,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2017,9]]}},"alternative-id":["10.1111\/jop.12528"],"URL":"https:\/\/doi.org\/10.1111\/jop.12528","archive":["Portico"],"relation":{},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,1,16]]}}}