{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,17]],"date-time":"2026-10-17T15:14:01Z","timestamp":1792250041851,"version":"4.4.0"},"reference-count":24,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,1,24]],"date-time":"2017-01-24T00:00:00Z","timestamp":1485216000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2017,7]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Monocyte chemoattractant protein\u20101 (CCL2) is a major profibrotic mediator with a proven role in fibrosis of different organs of the body. Recently, increased fibrosis in oral submucous fibrosis (OSMF) is linked with betel quid\u2010related chronic irritation and myofibroblast. Hence, this study is designed to investigate expression of CCL2 in OSMF and its correlation with myofibroblasts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Paraffin\u2010embedded specimens of 30 OSMF and 10 tissues of normal buccal mucosa were subjected to immunohistochemical analysis for CCL2 and alpha\u2010smooth muscle actin (\u03b1\u2010SMA) expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CCL2 expression in basal cells (CCL2\u2010B) and connective tissue (CCL2\u2010CT), and \u03b1\u2010SMA showed significantly increased expression in advanced OSMF as compared with early OSMF and controls. Significant differences were observed in the expression of CCL2\u2010B between control vs. OSMF (<jats:italic>P<\/jats:italic> = 0.002), control vs. advanced OSMF (<jats:italic>P<\/jats:italic> = 0.005), and early vs. advanced OSMF (<jats:italic>P<\/jats:italic> = 0.0377). Similarly, differences in the CCL2\u2010CT expression were statistically significant between control vs. OSMF (<jats:italic>P<\/jats:italic> = 0.00086), control vs. early OSMF (<jats:italic>P<\/jats:italic> = 0.02914), and control vs. advanced OSMF (<jats:italic>P<\/jats:italic> = 0.0006). For \u03b1\u2010SMA expression, significant differences were observed between control vs. OSMF (<jats:italic>P<\/jats:italic> = 0.0003), control vs. early OSMF (<jats:italic>P<\/jats:italic> = 0.036), control vs. advanced OSMF (<jats:italic>P<\/jats:italic> = 0.00008), and early vs. advanced OSMF (<jats:italic>P<\/jats:italic> = 0.0009). In OSMF group, a significant correlation was observed between CCL2\u2010B and CCL2\u2010CT (<jats:italic>P<\/jats:italic> &lt; 0.00001), CCL2\u2010B and \u03b1\u2010SMA (<jats:italic>P<\/jats:italic> &lt; 0.00001), and CCL2\u2010CT and \u03b1\u2010SMA (<jats:italic>P<\/jats:italic> &lt; 0.00001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CCL2 could be responsible for pathogenesis of OSMF by recruiting myofibroblasts.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12543","type":"journal-article","created":{"date-parts":[[2016,12,28]],"date-time":"2016-12-28T14:58:18Z","timestamp":1482937098000},"page":"443-447","source":"Crossref","is-referenced-by-count":18,"title":["Myofibroblasts could be recruited in a chemokine (C\u2010C motif) ligand 2\u2010dependent manner in pathogenesis of oral submucous fibrosis"],"prefix":"10.1111","volume":"46","author":[{"given":"Gargi","family":"Sarode","sequence":"first","affiliation":[{"name":"Department of Oral Pathology and Microbiology Dr. D. Y. Patil Dental College and Hospital Dr. D.Y. Patil Vidyapeeth Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1856-0957","authenticated-orcid":false,"given":"Sachin C.","family":"Sarode","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Microbiology Dr. D. Y. Patil Dental College and Hospital Dr. D.Y. Patil Vidyapeeth Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Revati","family":"Deshmukh","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Microbiology Dr. D. Y. Patil Dental College and Hospital Dr. D.Y. Patil Vidyapeeth Pune Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prashant","family":"Raktade","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery Pandit Deendayal Upadhay Dental College and Hospital Solapur Maharashtra India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shankargouda","family":"Patil","sequence":"additional","affiliation":[{"name":"Department of Maxillofacial Surgery and Diagnostic Sciences Division of Oral Pathology College of Dentistry Jazan University Jazan Saudi Arabia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,1,24]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2005.08.005"},{"key":"e_1_2_6_3_1","doi-asserted-by":"crossref","first-page":"499","DOI":"10.4103\/0973-1482.137958","article-title":"Betel nut chewing and its deleterious effects on oral cavity","volume":"10","author":"Anand R","year":"2014","journal-title":"J Can Res Ther"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/0140-6736(92)92109-S"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2010.00995.x"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oooo.2016.04.003"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2277"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200712-1827OC"},{"key":"e_1_2_6_9_1","first-page":"ZC14","article-title":"Evaluation of myofibroblasts by expression of alpha smooth muscle actin: a marker in fibrosis, dysplasia and carcinoma","volume":"8","author":"Rao KB","year":"2014","journal-title":"J Clin Diagn Res"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.4103\/0019-509X.175580"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.4103\/0973-1482.147700"},{"key":"e_1_2_6_12_1","first-page":"55","article-title":"An overview of classification schemes for oral submucous fibrosis","volume":"10","author":"Ranganathan K","year":"2006","journal-title":"J Oral Maxillofac Pathol"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2559.2001.01211.x"},{"key":"e_1_2_6_14_1","first-page":"375","article-title":"RelB regulation of chemokine expression modulates local inflammation","volume":"151","author":"Xia Y","year":"1997","journal-title":"Am J Pathol"},{"key":"e_1_2_6_15_1","doi-asserted-by":"crossref","first-page":"2193","DOI":"10.4049\/jimmunol.163.4.2193","article-title":"Differential monocyte chemoattractant protein\u20101 and chemokine receptor 2 expression by murine lung fibroblasts derived from Th1\u2010 and Th2\u2010type pulmonary granuloma models","volume":"163","author":"Hogaboam CM","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1084\/jem.185.7.1371"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2012.11.004"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200106)44:6<1382::AID-ART231>3.0.CO;2-T"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(00)00169-9"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200111)44:11<2665::AID-ART446>3.0.CO;2-S"},{"key":"e_1_2_6_21_1","first-page":"372","article-title":"Promotion of leukocyte transendothelial cell migration by chemokines derived from human biliary epithelial cells in vitro","volume":"109","author":"Morland CM","year":"1997","journal-title":"Proc Assoc Am Physicians"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00308.2005"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.153.10.4733"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/jlb.55.1.120"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00336.2003"}],"container-title":["Journal of Oral Pathology &amp; 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