{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T04:18:07Z","timestamp":1791001087319,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2017,2,11]],"date-time":"2017-02-11T00:00:00Z","timestamp":1486771200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["NSFC81672659, NSFC81472523, NSFC81272953"],"award-info":[{"award-number":["NSFC81672659, NSFC81472523, NSFC81272953"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003453","name":"Natural Science Foundation of Guangdong Province","doi-asserted-by":"publisher","award":["2015A030313017"],"award-info":[{"award-number":["2015A030313017"]}],"id":[{"id":"10.13039\/501100003453","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2017,10]]},"abstract":"Background<\/jats:title>AKT<\/jats:styled-content>1, also known as PKB<\/jats:styled-content>\u03b1, is abnormally expressed in various malignancies. In this study, we aimed to evaluate the role of AKT<\/jats:styled-content>1 in the tongue squamous cell carcinoma (TSCC<\/jats:styled-content>) and further clarify the mechanisms of AKT<\/jats:styled-content>1 in the migration and invasion of TSCC<\/jats:styled-content>.<\/jats:p><\/jats:sec>Methods<\/jats:title>At first, immunohistochemistry (IHC<\/jats:styled-content>) was conducted to detect the expression of AKT<\/jats:styled-content>1 in TSCC<\/jats:styled-content>. Then, we determined the role of AKT<\/jats:styled-content>1 in the migration and invasion of TSCC<\/jats:styled-content> and further investigated whether AKT<\/jats:styled-content>1 was the target gene of miR\u2010138 using dual luciferase reporter assays and Western blot.<\/jats:p><\/jats:sec>Results<\/jats:title>Immunohistochemistry results suggested that AKT<\/jats:styled-content>1 dysregulation was a frequent event in TSCC<\/jats:styled-content>, and upregulation of AKT<\/jats:styled-content>1 was correlated with lymph node metastasis and associated with reduced overall survival. UM<\/jats:styled-content>1 cells with higher migratory and invasive abilities had more robust AKT<\/jats:styled-content>1 protein expression than UM<\/jats:styled-content>2 cells with lower migratory and invasive abilities. The migration and invasion abilities were inhibited in UM<\/jats:styled-content>1 cells upon AKT<\/jats:styled-content>1 knockdown, meanwhile resulted in a decline of metastasis\u2010related proteins (vimentin, slug, and pERK<\/jats:styled-content>1\/2), and upregulation of E\u2010cadherin. Luciferase assays revealed that AKT<\/jats:styled-content>1 was directly targeted by miR\u2010138, and ectopic transfection of miR\u2010138 reduced the expression of AKT<\/jats:styled-content>1 protein.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>Our results confirm that upregulation of AKT<\/jats:styled-content>1, a miR\u2010138 target gene, is a frequent event in TSCC<\/jats:styled-content> and contributes to the aggressive behaviors and poor prognosis of TSCC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12551","type":"journal-article","created":{"date-parts":[[2017,1,25]],"date-time":"2017-01-25T18:03:20Z","timestamp":1485367400000},"page":"731-737","source":"Crossref","is-referenced-by-count":21,"title":["Dysregulation of AKT<\/scp>1, a miR\u2010138 target gene, is involved in the migration and invasion of tongue squamous cell carcinoma"],"prefix":"10.1111","volume":"46","author":[{"given":"Muyuan","family":"Ji","sequence":"first","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery The First Affiliated Hospital of Sun Yat\u2010Sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery The First Affiliated Hospital of Sun Yat\u2010Sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wangxiang","family":"Yan","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery The First Affiliated Hospital of Sun Yat\u2010Sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Oral and Maxillofacial Surgery The First Affiliated Hospital of Sun Yat\u2010Sen 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