{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:14:22Z","timestamp":1787015662317,"version":"build-2736575974"},"reference-count":35,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,7,11]],"date-time":"2017-07-11T00:00:00Z","timestamp":1499731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001782","name":"University of Melbourne","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001782","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2017,11]]},"abstract":"\n Background<\/jats:title>\n \n Subjective histopathology is currently used to diagnose oral squamous cell carcinoma (\n OSCC<\/jats:styled-content>\n ). We tested if abundances of a panel of micro\n RNA<\/jats:styled-content>\n could be an objective\n OSCC<\/jats:styled-content>\n indicator.\n <\/jats:p>\n <\/jats:sec>\n \n Method<\/jats:title>\n \n Literature review enabled identification of 10 micro\n RNA<\/jats:styled-content>\n s associated with oral and head and neck malignancies. We extracted\n RNA<\/jats:styled-content>\n from formalin\u2010fixed paraffin\u2010embedded biopsies; 20 each with\n OSCC<\/jats:styled-content>\n , dysplasia, or histologically normal epithelium (\n HNE<\/jats:styled-content>\n ) and 10 with oral lichen planus (\n OLP<\/jats:styled-content>\n ). Relative abundances of micro\n RNA<\/jats:styled-content>\n s in\n HNE<\/jats:styled-content>\n and\n OSCC<\/jats:styled-content>\n were determined using reverse transcription and then real\u2010time\n PCR<\/jats:styled-content>\n with global mean normalization. Micro\n RNA<\/jats:styled-content>\n s differentially expressed (test micro\n RNA<\/jats:styled-content>\n , T\u2010miR) and non\u2010differentially expressed (normalization micro\n RNA<\/jats:styled-content>\n , N\u2010miR) were identified. The raw micro\n RNA<\/jats:styled-content>\n Cq data were incorporated in a developed algorithm that output a T\u2010miR expression value (T\u2010mi\n REV<\/jats:styled-content>\n ) score. Raw Cq data from\n HNE<\/jats:styled-content>\n ,\n OSCC<\/jats:styled-content>\n , dysplasia, and\n OLP<\/jats:styled-content>\n samples were then used to test the algorithm scoring and\n OSCC<\/jats:styled-content>\n classification.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Four test and normalization micro\n RNA<\/jats:styled-content>\n s were identified. Algorithm output of T\u2010mir\n EV<\/jats:styled-content>\n >1 or <\u22121 indicated high and low\n OSCC<\/jats:styled-content>\n probability score, respectively, and gave 88.9% sensitivity, 100% specificity, and 93.5% accuracy. Grouping high and intermediate T\u2010mir\n EV<\/jats:styled-content>\n scores (T\u2010mi\n REV<\/jats:styled-content>\n \u2265\u22121) resulted in sensitivity of 90%, specificity of 65%, and accuracy of 77.5% in\n OSCC<\/jats:styled-content>\n classification. All 20 dysplasias and eight of 10\n OLP<\/jats:styled-content>\n had T\u2010mi\n REV<\/jats:styled-content>\n \u2265\u22121 indicating intermediate to high probability of malignant changes.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n A micro\n RNA<\/jats:styled-content>\n panel combined with our algorithm can identify tissue with probable oncogenic changes.\n <\/jats:p>\n <\/jats:sec>\n \n Impact<\/jats:title>\n The developed algorithm serves as a baseline for prospective trials, which may result in potential clinical utility.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jop.12592","type":"journal-article","created":{"date-parts":[[2017,5,17]],"date-time":"2017-05-17T03:16:53Z","timestamp":1494991013000},"page":"940-948","source":"Crossref","is-referenced-by-count":15,"title":["A panel of micro\n RNA<\/scp>\n s can be used to determine oral squamous cell carcinoma"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1316-7091","authenticated-orcid":false,"given":"Gareema","family":"Prasad","sequence":"first","affiliation":[{"name":"Melbourne Dental School The University of Melbourne Melbourne Vic. Australia"},{"name":"Oral Health Cooperative Research Centre The University of Melbourne Melbourne Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine","family":"Seers","sequence":"additional","affiliation":[{"name":"Melbourne Dental School The University of Melbourne Melbourne Vic. Australia"},{"name":"Oral Health Cooperative Research Centre The University of Melbourne Melbourne Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eric","family":"Reynolds","sequence":"additional","affiliation":[{"name":"Melbourne Dental School The University of Melbourne Melbourne Vic. Australia"},{"name":"Oral Health Cooperative Research Centre The University of Melbourne Melbourne Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael J.","family":"McCullough","sequence":"additional","affiliation":[{"name":"Melbourne Dental School The University of Melbourne Melbourne Vic. Australia"},{"name":"Oral Health Cooperative Research Centre The University of Melbourne Melbourne Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2005.08.011"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/154411130301400105"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2008.01.002"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2013.03.183"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2011.03.019"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2013.01.001"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1177\/2211068214561788"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-08-3131"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i34.12007"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddp446"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki863"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.03.201"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-0666"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2007.00584.x"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1593\/tlo.08163"},{"key":"e_1_2_7_17_1","first-page":"e102","article-title":"Oral lichen planus: a report and review of an automimmune\u2010mediated condition in gingiva","volume":"33","author":"Pendyala G","year":"2012","journal-title":"Compend Contin Educ Dent."},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1468-3083.2011.04306.x"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1593\/tlo.08163"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddp446"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1093\/carcin\/bgp277"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-0666"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2007.03.201"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.23831"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2009.080731"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-5194"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-08-3131"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/hed.21017"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1186\/1756-9966-28-64"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-09-0736"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1177\/154405910808701116"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-08-3053"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/gcc.20666"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki863"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0801130105"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6604948"}],"container-title":["Journal of Oral Pathology & Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12592","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.12592","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T17:44:27Z","timestamp":1696009467000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jop.12592"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,7,11]]},"references-count":35,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2017,11]]}},"alternative-id":["10.1111\/jop.12592"],"URL":"https:\/\/doi.org\/10.1111\/jop.12592","archive":["Portico"],"relation":{},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,7,11]]}}}