{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,18]],"date-time":"2026-07-18T00:40:11Z","timestamp":1784335211658,"version":"3.55.0"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,7,16]],"date-time":"2017-07-16T00:00:00Z","timestamp":1500163200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Medical Scientific Research Foundation of Guangdong Province","award":["A2014106"],"award-info":[{"award-number":["A2014106"]}]},{"name":"Science and Technology Project of Guangdong Province","award":["2013B021800196"],"award-info":[{"award-number":["2013B021800196"]}]},{"name":"Medical Technological Foundation of Guangzhou City","award":["20161A011088"],"award-info":[{"award-number":["20161A011088"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>This study sought to study the expression of the long non\u2010coding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (lnc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>) taurine\u2010upregulated gene 1 (<jats:styled-content style=\"fixed-case\">TUG<\/jats:styled-content>1) in tongue squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>) and reveal its possible function.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> was used to evaluate 27 samples of fresh <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> tissues and adjacent normal tongue tissues. si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> technology was employed to downregulate <jats:styled-content style=\"fixed-case\">TUG<\/jats:styled-content>1 expression in <jats:styled-content style=\"fixed-case\">CAL<\/jats:styled-content>\u201027 and <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>\u20109 cell lines. The 3\u2010(4,5\u2010dimethyl\u20102\u2010thiazolyl)\u20102,5\u2010diphenyl\u20102\u2010H\u2010tetrazolium bromide (<jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content>) assay was utilized to assess cell proliferation ability; apoptosis and cell\u2010cycle phases were analysed via flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> findings indicated that the lnc<jats:styled-content style=\"fixed-case\">RNA TUG<\/jats:styled-content>1 was upregulated in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content> tissues compared with adjacent normal tongue tissues (<jats:italic>P<\/jats:italic>&lt;.05). After <jats:styled-content style=\"fixed-case\">TUG<\/jats:styled-content>1 expression was downregulated using si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> technology, cell proliferation was significantly inhibited (<jats:italic>P<\/jats:italic>&lt;.05), and the number of cells in S phase was reduced (<jats:italic>P<\/jats:italic>&lt;.05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The lnc<jats:styled-content style=\"fixed-case\">RNA TUG<\/jats:styled-content>1 may represent a potential oncogene in <jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12599","type":"journal-article","created":{"date-parts":[[2017,6,9]],"date-time":"2017-06-09T11:49:02Z","timestamp":1497008942000},"page":"956-960","source":"Crossref","is-referenced-by-count":11,"title":["An in\u00a0vitro study of the long non\u2010coding <scp>RNA TUG<\/scp>1 in tongue squamous cell 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