{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T14:20:16Z","timestamp":1780928416738,"version":"3.54.1"},"reference-count":29,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,7,9]],"date-time":"2017-07-09T00:00:00Z","timestamp":1499558400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>\u03b2<jats:styled-content style=\"fixed-case\">III<\/jats:styled-content>\u2010tubulin (<jats:styled-content style=\"fixed-case\">TUBB<\/jats:styled-content>3) is an isotype of microtubules, which are involved in crucial cellular roles including maintenance of cell shape, intracellular transport, and mitosis. Overexpression of <jats:styled-content style=\"fixed-case\">TUBB<\/jats:styled-content>3 was found to be associated with poor prognosis and resistance to tubulin\u2010binding drugs and in several solid tumors including head and neck squamous cell carcinomas (<jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>). Considering the potential high importance of a prognostic biomarker in these cancers, this study aimed to investigate the clinical relevance of immunohistochemical <jats:styled-content style=\"fixed-case\">TUBB<\/jats:styled-content>3 expression in <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tissue microarray (<jats:styled-content style=\"fixed-case\">TMA<\/jats:styled-content>) sections containing samples from 667 cancers of oral cavity, oro\u2010 and hypopharynx, and larynx for which follow\u2010up data were available were analyzed for <jats:styled-content style=\"fixed-case\">TUBB<\/jats:styled-content>3 expression by immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Over 90% of our analyzed cancers showed unequivocal cytoplasmic <jats:styled-content style=\"fixed-case\">TUBB<\/jats:styled-content>3 expression. Staining was considered weak in 69 (15.5%), moderate in 149 (33.5%), and strong in 188 (42.2%) of cancers. The frequent <jats:styled-content style=\"fixed-case\">TUBB<\/jats:styled-content>3 overexpression showed no significant correlation with pathological grading, tumor stage, nodal status, or surgical margin and had no impact on patient outcomes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Despite lacking prognostic utility in <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content>, the remarkable high prevalence of <jats:styled-content style=\"fixed-case\">TUBB<\/jats:styled-content>3 expression in <jats:styled-content style=\"fixed-case\">HNSCC<\/jats:styled-content> emphasizes its putative relevance as a target for future drugs targeting <jats:styled-content style=\"fixed-case\">TUBB<\/jats:styled-content>3.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12607","type":"journal-article","created":{"date-parts":[[2017,6,22]],"date-time":"2017-06-22T16:52:39Z","timestamp":1498150359000},"page":"986-990","source":"Crossref","is-referenced-by-count":21,"title":["High\u2010level \u03b2<scp>III<\/scp>\u2010tubulin overexpression occurs in most head and neck cancers but is unrelated to clinical outcome"],"prefix":"10.1111","volume":"46","author":[{"given":"Julie C.","family":"Nienstedt","sequence":"first","affiliation":[{"name":"Center for Clinical Neurosciences Department of Voice, Speech and Hearing Disorders University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2822-2006","authenticated-orcid":false,"given":"Alexander","family":"Gr\u00f6be","sequence":"additional","affiliation":[{"name":"Center for Clinical Neurosciences, Oral &amp; 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