{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T06:10:16Z","timestamp":1769148616432,"version":"3.49.0"},"reference-count":30,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2017,7,25]],"date-time":"2017-07-25T00:00:00Z","timestamp":1500940800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The binary system of oral epithelial dysplasia (<jats:styled-content style=\"fixed-case\">OED<\/jats:styled-content>) has never been investigated with reference to the carcinogenesis\u2010related biomarkers. Hence, Ki67, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>105, and \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> immune\u2010expressions were studied in oral potentially malignant disorders (<jats:styled-content style=\"fixed-case\">OPMD<\/jats:styled-content>s) to assess their relationship with the binary <jats:styled-content style=\"fixed-case\">OED<\/jats:styled-content> grading system of World Health Organization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study was carried out on paraffin\u2010embedded tissues of 30 normal oral mucosa (<jats:styled-content style=\"fixed-case\">NOM<\/jats:styled-content>) and 140 <jats:styled-content style=\"fixed-case\">OPMD<\/jats:styled-content> cases. <jats:styled-content style=\"fixed-case\">OPMD<\/jats:styled-content> cases were classified into two groups \u201cno\/questionable\/hyperkeratosis\/mild\u201d=low\u2010risk epithelial dysplasia (<jats:styled-content style=\"fixed-case\">LRED<\/jats:styled-content>) and \u201cmoderate or severe\u201d=high\u2010risk epithelial dysplasia (<jats:styled-content style=\"fixed-case\">HRED<\/jats:styled-content>). The immunohistochemistry was carried out to evaluate the expression of Ki67, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content> 105, and \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> antigen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>According to the binary grading system of <jats:styled-content style=\"fixed-case\">WHO<\/jats:styled-content>, 69 (49.28%) cases were <jats:styled-content style=\"fixed-case\">LRED<\/jats:styled-content>, while 71 (50.71%) case showed <jats:styled-content style=\"fixed-case\">HRED<\/jats:styled-content>. There was significant increase in Ki67 labeling index (<jats:styled-content style=\"fixed-case\">LI<\/jats:styled-content>) from <jats:styled-content style=\"fixed-case\">NOM<\/jats:styled-content> to <jats:styled-content style=\"fixed-case\">LRED<\/jats:styled-content> to <jats:styled-content style=\"fixed-case\">HRED<\/jats:styled-content> (<jats:italic>P<\/jats:italic>=.000). Similarly, mean vascular density (<jats:styled-content style=\"fixed-case\">MVD<\/jats:styled-content>) also increased significantly from <jats:styled-content style=\"fixed-case\">NOM<\/jats:styled-content> to <jats:styled-content style=\"fixed-case\">LRED<\/jats:styled-content> to <jats:styled-content style=\"fixed-case\">HRED<\/jats:styled-content> (<jats:italic>P<\/jats:italic>=.000). The \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> expression was significantly higher in <jats:styled-content style=\"fixed-case\">HERD<\/jats:styled-content> compared to <jats:styled-content style=\"fixed-case\">LRED<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">NOM<\/jats:styled-content> (<jats:italic>P<\/jats:italic>=.000). A positive correlation was noted among Ki67 <jats:styled-content style=\"fixed-case\">LI<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> MVD<\/jats:styled-content>, and \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> expressions in <jats:styled-content style=\"fixed-case\">NOM<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> LRED<\/jats:styled-content>, and HRED (<jats:italic>P<\/jats:italic>=.000).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The expressions of ki67, <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>105, and \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> markers compliment binary grading system of <jats:styled-content style=\"fixed-case\">OED<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">OPMDS<\/jats:styled-content>, thus justifying its use in clinical practice.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12612","type":"journal-article","created":{"date-parts":[[2017,7,3]],"date-time":"2017-07-03T17:53:23Z","timestamp":1499104403000},"page":"921-927","source":"Crossref","is-referenced-by-count":17,"title":["Ki67, <scp>CD<\/scp>105, and \u03b1\u2010<scp>SMA<\/scp> expressions better relate the binary oral epithelial dysplasia grading system of World Health Organization"],"prefix":"10.1111","volume":"46","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6546-7694","authenticated-orcid":false,"given":"Amol Ramchandra","family":"Gadbail","sequence":"first","affiliation":[{"name":"Department of Dentistry Indira Gandhi Government Medical College and Hospital Nagpur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Minal S.","family":"Chaudhary","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Microbiology Sharad Pawar Dental College &amp; Hospital Datta Meghe Institute of Medical Sciences Sawangi (M) Wardha Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1856-0957","authenticated-orcid":false,"given":"Sachin C.","family":"Sarode","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Microbiology Dr. D.Y. Patil Dental College and Hospital Pune Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madhuri","family":"Gawande","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Microbiology Sharad Pawar Dental College &amp; Hospital Datta Meghe Institute of Medical Sciences Sawangi (M) Wardha Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheetal","family":"Korde","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Microbiology Yerala Dental College and Hospital Kharghar Mumbai India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2288-5167","authenticated-orcid":false,"given":"Satyajit Ashok","family":"Tekade","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Microbiology Modern Dental College &amp; Research Centre Indore Madhya Pradesh India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shailesh","family":"Gondivkar","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine and Radiology Government Dental College &amp; Hospital Nagpur Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8576-7897","authenticated-orcid":false,"given":"Alka","family":"Hande","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Microbiology Sharad Pawar Dental College &amp; Hospital Datta Meghe Institute of Medical Sciences Sawangi (M) Wardha Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rupesh","family":"Maladhari","sequence":"additional","affiliation":[{"name":"Department of Oral Pathology and Microbiology Sharad Pawar Dental College &amp; Hospital Datta Meghe Institute of Medical Sciences Sawangi (M) Wardha Maharashtra India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,7,25]]},"reference":[{"key":"e_1_2_7_2_1","volume-title":"Histological Grading of Oral Epithelial Atypia by the Use of Photographic Standards","author":"Smith CJ","year":"1969"},{"key":"e_1_2_7_3_1","first-page":"177","volume-title":"Pathology & Genetics. 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