{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T13:42:39Z","timestamp":1790602959272,"version":"4.1.0"},"reference-count":24,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T00:00:00Z","timestamp":1508716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Key Disciplines Constructional Project Funding, China"},{"name":"Nanjing Municipal Key Medical Laboratory Constructional Project Funding (Since 2012)"},{"name":"Center of Nanjing Clinical Medicine Tumor (Since 2014)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2018,1]]},"abstract":"<jats:sec><jats:title>Objectives<\/jats:title><jats:p>Margin status and invasion pattern are prognostic factors for oral tongue squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">OTSCC<\/jats:styled-content>). Current methods to identify these factors are limited to 2<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content> observation; it is necessary to explore 3<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content> reconstruction with whole\u2010mount sample to improve the accuracy of analysis. This study aimed to study the tissue preparation, section generation, and 3<jats:styled-content style=\"fixed-case\">D<\/jats:styled-content> reconstruction with whole\u2010mount <jats:styled-content style=\"fixed-case\">OTSCC<\/jats:styled-content> specimen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Study design<\/jats:title><jats:p>Two <jats:styled-content style=\"fixed-case\">OTSCC<\/jats:styled-content> samples were retrieved from Nanjing Stomatological Hospital, Medical School of Nanjing University. One sample was sliced into 3 equal\u2010sized pieces and subjected to different processing schedules to determine the best method. The second sample was processed accordingly. Serial whole\u2010mount sections of the second sample were generated, stained with <jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content>\/anticytokine antibody in intersection manner, and scanned into digital images. Digital images were aligned and reconstructed into 3D images with Hetero Genius Medical Image Manager 3D Pathology Add\u2010On [<jats:styled-content style=\"fixed-case\">HGMIM<\/jats:styled-content>3D].<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Successful serial whole\u2010mount sections of comparable quality to traditional sections were generated. Three\u2010dimensional images with serial whole\u2010mount sections were successfully generated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Whole\u2010mount histopathological 3D reconstruction of <jats:styled-content style=\"fixed-case\">OTSCC<\/jats:styled-content> was successfully generated, providing a solid foundation for comprehensive margin and invasion analysis. Although future study and improvement were needed, whole\u2010mount histopathological 3D reconstruction proved to be a promising method in <jats:styled-content style=\"fixed-case\">OTSCC<\/jats:styled-content> study.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12644","type":"journal-article","created":{"date-parts":[[2017,9,28]],"date-time":"2017-09-28T16:28:35Z","timestamp":1506616115000},"page":"53-59","source":"Crossref","is-referenced-by-count":5,"title":["Three\u2010dimensional reconstruction with serial whole\u2010mount sections of oral tongue squamous cell carcinoma: A preliminary study"],"prefix":"10.1111","volume":"47","author":[{"given":"Yujia","family":"Wang","sequence":"first","affiliation":[{"name":"Nangjing Stomatological Hospital Medical School of Nanjing University Nanjing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheng","family":"Chen","sequence":"additional","affiliation":[{"name":"Nangjing Stomatological Hospital Medical School of Nanjing 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