{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:12:33Z","timestamp":1787015553454,"version":"build-2736575974"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,11,17]],"date-time":"2017-11-17T00:00:00Z","timestamp":1510876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81000446"],"award-info":[{"award-number":["81000446"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81500848"],"award-info":[{"award-number":["81500848"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81630025"],"award-info":[{"award-number":["81630025"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81771070"],"award-info":[{"award-number":["81771070"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2018,2]]},"abstract":"\n Background<\/jats:title>\n \n We recently reported that the\n CC<\/jats:styled-content>\n chemokine ligand 2 (\n CCL<\/jats:styled-content>\n 2)\u2010\n CC<\/jats:styled-content>\n receptor 2 (\n CCR<\/jats:styled-content>\n 2) axis was involved in the pathogenesis of oral lichen planus (\n OLP<\/jats:styled-content>\n ). However, the exact mechanism for the high expression of\n CCL<\/jats:styled-content>\n 2 in\n OLP<\/jats:styled-content>\n specimens is not clear. Therefore, this study was designed to investigate the potential role of the toll\u2010like receptor 4 (\n TLR<\/jats:styled-content>\n \u20104) pathway in overproduction of\n CCL<\/jats:styled-content>\n 2 in\n OLP<\/jats:styled-content>\n lesions.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n Immunohistochemical staining and real\u2010time\n RT<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n were used to detect\n TLR<\/jats:styled-content>\n \u20104,\n CCL<\/jats:styled-content>\n 2, and\n CCR<\/jats:styled-content>\n 2 expression in\n OLP<\/jats:styled-content>\n lesions. Then, gingival epithelial cells from\n OLP<\/jats:styled-content>\n lesions were established and treated with\n Porphyromonas gingivalis<\/jats:italic>\n (\n P.\u00a0gingivalis)<\/jats:italic>\n lipopolysaccharide (\n LPS<\/jats:styled-content>\n ).\n CCL<\/jats:styled-content>\n 2 expression in epithelial cells was determined by Western blotting and real\u2010time\n RT<\/jats:styled-content>\n \u2010\n PCR<\/jats:styled-content>\n . In some experiments,\n TAK<\/jats:styled-content>\n \u2010242, a specific inhibitor of\n TLR<\/jats:styled-content>\n \u20104, was used to block the\n TLR<\/jats:styled-content>\n \u20104 pathway before cells were stimulated with\n LPS<\/jats:styled-content>\n .\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n We found that\n TLR<\/jats:styled-content>\n \u20104 was significantly increased in the epithelium of\n OLP<\/jats:styled-content>\n specimens, compared with controls. Moreover,\n LPS<\/jats:styled-content>\n can induce the over production of\n CCL<\/jats:styled-content>\n 2 in epithelial cells of\n OLP<\/jats:styled-content>\n , in\u00a0vitro.\n TAK<\/jats:styled-content>\n \u2010242 effectively eliminated the increase in\n CCL<\/jats:styled-content>\n 2 expression induced by\n LPS<\/jats:styled-content>\n by blocking the\n TLR<\/jats:styled-content>\n \u20104\/\n NF<\/jats:styled-content>\n \u2010\u03baB pathway. In addition, we again confirmed that expression of\n CCL<\/jats:styled-content>\n 2 and\n CCR<\/jats:styled-content>\n 2 was increased in\n OLP<\/jats:styled-content>\n specimens.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n \n Increased\n TLR<\/jats:styled-content>\n \u20104 expression contributes to the upregulated expression of\n CCL<\/jats:styled-content>\n 2 in the epithelium of\n OLP<\/jats:styled-content>\n lesions, which suggests that oral bacteria participate in the pathogenesis of\n OLP<\/jats:styled-content>\n via the\n TLR<\/jats:styled-content>\n \u20104 pathway.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/jop.12655","type":"journal-article","created":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T04:26:38Z","timestamp":1508732798000},"page":"166-172","source":"Crossref","is-referenced-by-count":17,"title":["Porphyromonas gingivalis<\/i>\n lipopolysaccharide induces over production of\n CC<\/scp>\n chemokine ligand 2 via toll\u2010like receptor\u20104 in oral lichen planus"],"prefix":"10.1111","volume":"47","author":[{"given":"Qi","family":"Zeng","sequence":"first","affiliation":[{"name":"Department of Pediatric Dentistry Guanghua School of Stomatology Guangdong Provincial Key Laboratory of Stomatology Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Periodontology Stomatological Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaobing","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine Guanghua School of Stomatology Guangdong Provincial Key Laboratory of Stomatology Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan","family":"Xia","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine Guanghua School of Stomatology Guangdong Provincial Key Laboratory of Stomatology Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Cheng","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine Guanghua School of Stomatology Guangdong Provincial Key Laboratory of Stomatology Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1114-9698","authenticated-orcid":false,"given":"Xiaoan","family":"Tao","sequence":"additional","affiliation":[{"name":"Department of Oral Medicine Guanghua School of Stomatology Guangdong Provincial Key Laboratory of Stomatology Sun Yat\u2010sen University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,11,17]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.archoralbio.2013.04.004"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1177\/154411130201300405"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.2010.00946.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12090"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/odi.12094"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep29186"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep22943"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80119-3"},{"key":"e_1_2_6_10_1","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.coi.2016.10.005","article-title":"Early innate immune responses to bacterial 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