{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,27]],"date-time":"2025-06-27T07:30:28Z","timestamp":1751009428116,"version":"3.37.3"},"reference-count":20,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2017,12,18]],"date-time":"2017-12-18T00:00:00Z","timestamp":1513555200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100004663","name":"Ministry of Science and Technology, Taiwan","doi-asserted-by":"publisher","award":["NSC 100\u20102314\u2010B\u2010040\u2010005\u2010MY3"],"award-info":[{"award-number":["NSC 100\u20102314\u2010B\u2010040\u2010005\u2010MY3"]}],"id":[{"id":"10.13039\/501100004663","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">STRO<\/jats:styled-content>\u20101 is a mesenchymal cell marker present on all clonogenic stromal precursors. Current evidence has indicated that the pathogenesis of fibrotic changes may be mediated by stemness properties. The aim of this study was to investigate the role of <jats:styled-content style=\"fixed-case\">STRO<\/jats:styled-content>\u20101 in areca quid chewing\u2010associated oral submucous fibrosis (<jats:styled-content style=\"fixed-case\">OSF<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Thirty <jats:styled-content style=\"fixed-case\">OSF<\/jats:styled-content> specimens and ten normal buccal mucosae were examined by immunohistochemistry. The activity of STRO\u20101 from fibroblasts cultured from normal buccal mucosa (BMFs) and OSF (OSFFs) were measureed and the effect of arecoline, a major areca nut alkaloid, on STRO\u20101 in BMFs was evaluated. Compared the activities between sorted <jats:styled-content style=\"fixed-case\">STRO<\/jats:styled-content>\u20101<jats:sup>+<\/jats:sup> cells and <jats:styled-content style=\"fixed-case\">STRO<\/jats:styled-content>\u20101<jats:sup>\u2212<\/jats:sup> cells from <jats:styled-content style=\"fixed-case\">OSFF<\/jats:styled-content> were measured by collagen gel contraction, migration, invasion abilities, and the expression of \u03b1\u2010smooth muscle actin (\u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>) and pro\u2010\u03b11 (I) chain of type I collagen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Our results first showed that the expression of <jats:styled-content style=\"fixed-case\">STRO<\/jats:styled-content>\u20101 was more evident in areca quid chewing\u2010associated <jats:styled-content style=\"fixed-case\">OSF<\/jats:styled-content> than normal buccal mucosa tissues (<jats:italic>P<\/jats:italic> &lt; .05). Arecoline dose\u2010dependently activated the level of <jats:styled-content style=\"fixed-case\">STRO<\/jats:styled-content>\u20101 in <jats:styled-content style=\"fixed-case\">BMF<\/jats:styled-content>s (<jats:italic>P<\/jats:italic> &lt; .05). The relative expression of <jats:styled-content style=\"fixed-case\">STRO<\/jats:styled-content>\u20101 was significantly higher in <jats:styled-content style=\"fixed-case\">OSFF<\/jats:styled-content>s compared with <jats:styled-content style=\"fixed-case\">BMF<\/jats:styled-content>s (<jats:italic>P<\/jats:italic> &lt; .05). In addition, the sorted <jats:styled-content style=\"fixed-case\">STRO<\/jats:styled-content>\u20101<jats:sup>+<\/jats:sup> cells from <jats:styled-content style=\"fixed-case\">OSFF<\/jats:styled-content>s exhibited higher collagen gel contraction, migration, and invasion abilities as well as elevated expression of \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> and pro\u2010\u03b11 (I) chain of type I collagen than the negative subset (<jats:italic>P<\/jats:italic> &lt; .05).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings suggested that the stemness marker <jats:styled-content style=\"fixed-case\">STRO<\/jats:styled-content>\u20101 may be a crucial factor in the pathogenesis of areca quid chewing\u2010associated <jats:styled-content style=\"fixed-case\">OSF<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12668","type":"journal-article","created":{"date-parts":[[2017,12,6]],"date-time":"2017-12-06T06:32:46Z","timestamp":1512541966000},"page":"299-305","source":"Crossref","is-referenced-by-count":6,"title":["<scp>STRO<\/scp>\u20101 confers myofibroblast transdifferentiation in fibroblasts derived from oral submucous fibrosis"],"prefix":"10.1111","volume":"47","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2720-3145","authenticated-orcid":false,"given":"Cheng\u2010Chia","family":"Yu","sequence":"first","affiliation":[{"name":"Institute of Oral Sciences Chung Shan Medical University Taichung Taiwan"},{"name":"School of Dentistry Chung Shan Medical University Taichung Taiwan"},{"name":"Department of Dentistry Chung Shan Medical University Hospital Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Wen","family":"Liao","sequence":"additional","affiliation":[{"name":"School of Dentistry Chung Shan Medical University Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chuan\u2010Hang","family":"Yu","sequence":"additional","affiliation":[{"name":"School of Dentistry Chung Shan Medical University Taichung Taiwan"},{"name":"Department of Dentistry Chung Shan Medical University Hospital Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9731-2577","authenticated-orcid":false,"given":"Yu\u2010Chao","family":"Chang","sequence":"additional","affiliation":[{"name":"School of Dentistry Chung Shan Medical University Taichung Taiwan"},{"name":"Department of Dentistry Chung Shan Medical University Hospital Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,12,18]]},"reference":[{"doi-asserted-by":"publisher","key":"e_1_2_7_2_1","DOI":"10.1111\/jop.12570"},{"key":"e_1_2_7_3_1","article-title":"Changes in prevalence of pre\u2010cancerous oral submucous fibrosis from 1996\u20102013 in Taiwan: a nationwide population\u2010based retrospective study","author":"Yang SF","year":"2013","journal-title":"J Formos Med Assoc"},{"doi-asserted-by":"publisher","key":"e_1_2_7_4_1","DOI":"10.1080\/13556210020091437"},{"doi-asserted-by":"publisher","key":"e_1_2_7_5_1","DOI":"10.1016\/j.oooo.2016.04.003"},{"doi-asserted-by":"publisher","key":"e_1_2_7_6_1","DOI":"10.1111\/j.1600-0714.2010.00995.x"},{"doi-asserted-by":"publisher","key":"e_1_2_7_7_1","DOI":"10.1371\/journal.pone.0055122"},{"doi-asserted-by":"publisher","key":"e_1_2_7_8_1","DOI":"10.1111\/jcmm.12219"},{"doi-asserted-by":"publisher","key":"e_1_2_7_9_1","DOI":"10.18632\/oncotarget.7763"},{"doi-asserted-by":"publisher","key":"e_1_2_7_10_1","DOI":"10.1038\/srep37004"},{"doi-asserted-by":"publisher","key":"e_1_2_7_11_1","DOI":"10.1182\/blood.V78.1.55.55"},{"doi-asserted-by":"publisher","key":"e_1_2_7_12_1","DOI":"10.1016\/S0140-6736(04)16627-0"},{"doi-asserted-by":"publisher","key":"e_1_2_7_13_1","DOI":"10.1359\/jbmr.2003.18.4.696"},{"doi-asserted-by":"publisher","key":"e_1_2_7_14_1","DOI":"10.1016\/j.joen.2007.11.021"},{"doi-asserted-by":"publisher","key":"e_1_2_7_15_1","DOI":"10.1002\/jcp.22405"},{"doi-asserted-by":"publisher","key":"e_1_2_7_16_1","DOI":"10.4317\/jced.51305"},{"doi-asserted-by":"publisher","key":"e_1_2_7_17_1","DOI":"10.1002\/hed.23130"},{"doi-asserted-by":"publisher","key":"e_1_2_7_18_1","DOI":"10.1371\/journal.pone.0051806"},{"doi-asserted-by":"publisher","key":"e_1_2_7_19_1","DOI":"10.1038\/nm.1979"},{"doi-asserted-by":"publisher","key":"e_1_2_7_20_1","DOI":"10.1002\/hep.23544"},{"doi-asserted-by":"publisher","key":"e_1_2_7_21_1","DOI":"10.1038\/onc.2016.299"}],"container-title":["Journal of Oral Pathology &amp; 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