{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T12:24:04Z","timestamp":1789820644422,"version":"4.0.1"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,1,22]],"date-time":"2018-01-22T00:00:00Z","timestamp":1516579200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Enhancer of zeste homolog 2 (<jats:styled-content style=\"fixed-case\">EZH<\/jats:styled-content>2), the catalytic subunit of the polycomb repressive complex 2, plays an important role in tumor development and progression by interacting with histone and non\u2010histone proteins. <jats:styled-content style=\"fixed-case\">EZH<\/jats:styled-content>2 represents a putative therapeutic target and has been suggested as a prognostic marker in several cancer types.<\/jats:p><\/jats:sec><jats:sec><jats:title>Material and Methods<\/jats:title><jats:p>This study investigates the prognostic relevance of immunohistochemical <jats:styled-content style=\"fixed-case\">EZH<\/jats:styled-content>2 expression in head and neck squamous cell carcinoma. Tissue microarray sections with 667 cancers of oral cavity, oro\u2010 and hypopharynx and larynx were analyzed for <jats:styled-content style=\"fixed-case\">EZH<\/jats:styled-content>2 expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Nuclear <jats:styled-content style=\"fixed-case\">EZH<\/jats:styled-content>2 staining was recorded in 322 (81.8%) of 394 cases. Staining was weak in 33 (10.2%), moderate in 128 (39.6%), and strong in 103 (32.0%) cancers. The prevalence of <jats:styled-content style=\"fixed-case\">EZH<\/jats:styled-content>2 expression in tumors of the oral cavity and the orohypopharynx was higher as compared to cancers of the larynx (<jats:italic>P<\/jats:italic> = .0023). <jats:styled-content style=\"fixed-case\">EZH<\/jats:styled-content>2 expression was correlated to presence of lymph node metastasis (<jats:italic>P<\/jats:italic> = .0089) but was unrelated to histological grade, tumor stage, surgical margin, or distant metastasis. <jats:styled-content style=\"fixed-case\">EZH<\/jats:styled-content>2 expression had no impact on patient survival.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The high prevalence of <jats:styled-content style=\"fixed-case\">EZH<\/jats:styled-content>2 expression in head and neck squamous cell carcinoma stresses its capability as a therapeutic target.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12673","type":"journal-article","created":{"date-parts":[[2017,12,29]],"date-time":"2017-12-29T06:52:42Z","timestamp":1514530362000},"page":"240-245","source":"Crossref","is-referenced-by-count":23,"title":["<scp>EZH<\/scp>2 overexpression in head and neck cancer is related to lymph node metastasis"],"prefix":"10.1111","volume":"47","author":[{"given":"Julie C.","family":"Nienstedt","sequence":"first","affiliation":[{"name":"Department of Voice, Speech and Hearing Disorders Center for Clinical Neurosciences University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelia","family":"Schroeder","sequence":"additional","affiliation":[{"name":"Institute of Pathology University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"},{"name":"Department of General, Visceral and Thoracic Surgery Center for Surgical Sciences University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Till","family":"Clauditz","sequence":"additional","affiliation":[{"name":"Institute of Pathology University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0158-4258","authenticated-orcid":false,"given":"Ronald","family":"Simon","sequence":"additional","affiliation":[{"name":"Institute of Pathology University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guido","family":"Sauter","sequence":"additional","affiliation":[{"name":"Institute of Pathology University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrian","family":"Muenscher","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Center for Clinical Neurosciences University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marco","family":"Blessmann","sequence":"additional","affiliation":[{"name":"Department of Plastic, Reconstructive and Aesthetic Surgery Center for Surgical Sciences University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henning","family":"Hanken","sequence":"additional","affiliation":[{"name":"Center for Clinical Neurosciences, Oral &amp; Maxillofacial surgery University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christina","family":"Pflug","sequence":"additional","affiliation":[{"name":"Department of Voice, Speech and Hearing Disorders Center for Clinical Neurosciences University Medical Center Hamburg\u2010Eppendorf Hamburg Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,22]]},"reference":[{"key":"e_1_2_11_2_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.21208"},{"key":"e_1_2_11_3_1","doi-asserted-by":"publisher","DOI":"10.1159\/000048770"},{"key":"e_1_2_11_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2016.02.004"},{"key":"e_1_2_11_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2010.08.002"},{"key":"e_1_2_11_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4092"},{"key":"e_1_2_11_7_1","doi-asserted-by":"publisher","DOI":"10.2217\/epi.14.23"},{"key":"e_1_2_11_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1933744100"},{"key":"e_1_2_11_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature14122"},{"key":"e_1_2_11_10_1","doi-asserted-by":"publisher","DOI":"10.1200\/JCO.2005.01.5180"},{"key":"e_1_2_11_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2006.00203.x"},{"key":"e_1_2_11_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12943-014-0284-y"},{"key":"e_1_2_11_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6602531"},{"key":"e_1_2_11_14_1","doi-asserted-by":"publisher","DOI":"10.1159\/000087804"},{"key":"e_1_2_11_15_1","doi-asserted-by":"publisher","DOI":"10.5858\/arpa.2012-0525-OA"},{"key":"e_1_2_11_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3799"},{"key":"e_1_2_11_17_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms7051"},{"key":"e_1_2_11_18_1","doi-asserted-by":"publisher","DOI":"10.4132\/KoreanJPathol.2014.48.4.263"},{"key":"e_1_2_11_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2015.11.002"},{"key":"e_1_2_11_20_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.5606"},{"key":"e_1_2_11_21_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.21848"},{"key":"e_1_2_11_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12111"},{"key":"e_1_2_11_23_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.3180"},{"key":"e_1_2_11_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2008.03.016"},{"key":"e_1_2_11_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2016.09.055"},{"key":"e_1_2_11_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/cncr.26575"},{"key":"e_1_2_11_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature11606"},{"key":"e_1_2_11_28_1","doi-asserted-by":"publisher","DOI":"10.1158\/1940-6207.CAPR-11-0130"},{"key":"e_1_2_11_29_1","first-page":"1179","article-title":"Tissue microarray analysis reveals site\u2010specific prevalence of oncogene amplifications in head and neck squamous cell carcinoma","volume":"63","author":"Freier K","year":"2003","journal-title":"Cancer Res"},{"key":"e_1_2_11_30_1","first-page":"5213","article-title":"CCND1 amplification predicts sensitivity to chemotherapy and chemoradiotherapy in head and neck squamous cell carcinoma","volume":"23","author":"Ishiguro R","year":"2003","journal-title":"Anticancer Res"},{"key":"e_1_2_11_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.2910520405"},{"key":"e_1_2_11_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.4036"}],"container-title":["Journal of Oral Pathology &amp; Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjop.12673","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jop.12673","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,25]],"date-time":"2023-09-25T13:16:07Z","timestamp":1695647767000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jop.12673"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,22]]},"references-count":31,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,3]]}},"alternative-id":["10.1111\/jop.12673"],"URL":"https:\/\/doi.org\/10.1111\/jop.12673","archive":["Portico"],"relation":{},"ISSN":["0904-2512","1600-0714"],"issn-type":[{"value":"0904-2512","type":"print"},{"value":"1600-0714","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,22]]}}}