{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,6]],"date-time":"2025-10-06T09:05:28Z","timestamp":1759741528888,"version":"3.37.3"},"reference-count":30,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,6,19]],"date-time":"2018-06-19T00:00:00Z","timestamp":1529366400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100006756","name":"Brigham Young University","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100006756","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Gas6 protein is involved in the progression of cancers and has been demonstrated to have a role in inflammation. Oral squamous cell carcinoma is a common form of oral cancer, and it commonly expresses Gas6. Our objective was to determine the effects of Gas6 on oral squamous cell carcinoma invasion and identify signaling molecules and cytokines associated with Gas6\u2010mediated invasion.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Ca9\u201022 cells were cultured in the presence or absence of Gas6. Real\u2010time cell invasion was evaluated, and cultured cells were lysed for Western blot analysis. Cell medium was collected and assayed for cytokine elaboration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Treatment of cells with Gas6 resulted in: (i) increased invasion, (ii) increased expression of Gas6 and <jats:styled-content style=\"fixed-case\">AXL<\/jats:styled-content> receptor, (iii) reduced invasion when <jats:styled-content style=\"fixed-case\">AXL<\/jats:styled-content> was inhibited, (iv) decreased <jats:styled-content style=\"fixed-case\">ERK<\/jats:styled-content> activation, (v) increased <jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content> activation, and (vi) decreased secretion of G\u2010<jats:styled-content style=\"fixed-case\">CSF<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> IL<\/jats:styled-content>\u20102, <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106, and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Gas6 increases invasion of oral squamous cell carcinoma, and the invasion correlates with the increased <jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content> and the downregulation of pro\u2010inflammatory cytokines. These results may prove useful in providing avenues that explain the role of Gas6 in the development and progression of oral squamous cell carcinoma.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12738","type":"journal-article","created":{"date-parts":[[2018,6,1]],"date-time":"2018-06-01T09:41:08Z","timestamp":1527846068000},"page":"748-754","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Gas6 protein induces invasion and reduces inflammatory cytokines in oral squamous cell carcinoma"],"prefix":"10.1111","volume":"47","author":[{"given":"Kelsey M.","family":"Hirschi","sequence":"first","affiliation":[{"name":"Lung and Placenta Research Laboratory Department of Physiology and Developmental Biology Brigham Young University Provo UT USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Steven","family":"Chapman","sequence":"additional","affiliation":[{"name":"Lung and Placenta Research Laboratory 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