{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,10]],"date-time":"2026-02-10T19:42:51Z","timestamp":1770752571149,"version":"3.50.0"},"reference-count":23,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,9]],"date-time":"2018-12-09T00:00:00Z","timestamp":1544313600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100008903","name":"Ministry of Health and Welfare","doi-asserted-by":"publisher","award":["MOHW107\u2010TDU\u2010B\u2010212\u2010114025"],"award-info":[{"award-number":["MOHW107\u2010TDU\u2010B\u2010212\u2010114025"]}],"id":[{"id":"10.13039\/100008903","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Oral submucous fibrosis (OSF) is a progressive scarring disease and has been considered as a premalignant condition of the oral cavity. However, the detailed molecular mechanisms underlying the pathogenesis of OSF are still unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Here, we examined the expression of a novel long non\u2010coding RNA LINC00974 in OSF and investigated its function role in myofibroblast transdifferentiation. Phenotypic analyses, including collagen gel contraction, migration, invasion and wound healing assays, were used to assess the myofibroblast activities following overexpression or inhibition of LINC00974.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that the expression of LINC00974 in OSF tissues or myofibroblasts was aberrantly upregulated, and there was a positive correlation between LINC00974 and myofibroblast markers. Our results showed that inhibition of\u00a0LINC00974 suppressed the myofibroblast activities, while overexpression of LINC00974 increased the activation. We demonstrated that the expression levels of \u03b1\u2010SMA, \u03b1\u20101 type I collagen, fibronectin were downregulated in the LINC00974\u2010inhibited myofibroblasts. Additionally, the TGF\u2010\u03b2 secretion and phosphorylated Smad2 expression were also repressed in the LINC00974\u2010inhibited myofibroblasts. We further demonstrated that silence of LINC00974 prevented the arecoline\u2010induced myofibroblast activation, and LINC00974\u2010increased myofibroblast activities were via TGF\u2010\u03b2 pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Altogether, these findings suggested that arecoline\u2010increased myofibroblast transdifferentiation was via LINC00974\u2010mediated activation of TGF\u2010\u03b2 signaling.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12805","type":"journal-article","created":{"date-parts":[[2018,11,17]],"date-time":"2018-11-17T13:32:21Z","timestamp":1542461541000},"page":"151-158","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["LncRNA LINC00974 activates TGF\u2010\u03b2\/Smad signaling to promote oral fibrogenesis"],"prefix":"10.1111","volume":"48","author":[{"given":"Chih\u2010Yuan","family":"Fang","sequence":"first","affiliation":[{"name":"School of Dentistry College of Oral Medicine Taipei Medical University Taipei Taiwan"},{"name":"Division of Oral and Maxillofacial Surgery Department of Dentistry Wan Fang Hospital Taipei Medical University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2720-3145","authenticated-orcid":false,"given":"Cheng\u2010Chia","family":"Yu","sequence":"additional","affiliation":[{"name":"Institute of Oral Sciences Chung Shan Medical University Taichung Taiwan"},{"name":"Department of Dentistry Chung Shan Medical University Hospital Taichung Taiwan"},{"name":"School of Dentistry Chung Shan Medical University Taichung Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yi\u2010Wen","family":"Liao","sequence":"additional","affiliation":[{"name":"School of Dentistry Chung Shan Medical University Taichung 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Medical University Taipei Taiwan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6272-4835","authenticated-orcid":false,"given":"Lo\u2010Lin","family":"Tsai","sequence":"additional","affiliation":[{"name":"School of Dentistry College of Oral Medicine Taipei Medical University Taipei Taiwan"},{"name":"Division of Oral and Maxillofacial Surgery Department of Dentistry Wan Fang Hospital Taipei Medical University Taipei 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