{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,2]],"date-time":"2025-07-02T13:32:42Z","timestamp":1751463162859,"version":"3.37.3"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,30]],"date-time":"2018-12-30T00:00:00Z","timestamp":1546128000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81600899","81702695"],"award-info":[{"award-number":["81600899","81702695"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>The chemokine receptor 4 (<jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4) plays an important role in tumor progression. Overexpressed <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 is associated with a poor prognosis of patient with head and neck squamous cell carcinomas. However, the correlation between <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 and chemotherapy resistance in tongue squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">TSCC<\/jats:styled-content>) remains obscure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Stable cisplatin\u2010resistant <jats:styled-content style=\"fixed-case\">CAL<\/jats:styled-content>27 <jats:styled-content style=\"fixed-case\">CDDP<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>25 <jats:styled-content style=\"fixed-case\">CDDP<\/jats:styled-content> cells were established and identified by <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>8 assay, and the <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 expression was detected using <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and Western blot. <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4\u2010si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was transfected into <jats:styled-content style=\"fixed-case\">TSCC CDDP<\/jats:styled-content> cells, whose transfect efficiency was examined. Cisplatin sensitivity was further detected, as well as several proliferation and apoptosis\u2010related proteins.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">CAL<\/jats:styled-content>27 <jats:styled-content style=\"fixed-case\">CDDP<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>25 <jats:styled-content style=\"fixed-case\">CDDP<\/jats:styled-content> cells were successfully established, which exhibited significantly higher cell viability and less apoptosis under cisplatin stimulation than that of parental cells. <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 expression was increased in <jats:styled-content style=\"fixed-case\">TSCC CDDP<\/jats:styled-content> cells. After transfection of <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4\u2010si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>, the expression of <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 was reduced by 73% and 78% in <jats:styled-content style=\"fixed-case\">CAL<\/jats:styled-content>27 <jats:styled-content style=\"fixed-case\">CDDP<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SCC<\/jats:styled-content>25 <jats:styled-content style=\"fixed-case\">CDDP<\/jats:styled-content> cells, respectively. <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>8 assay and flow cytometry assay revealed that the proliferative capacity under cisplatin stimulation significantly decreased after <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 silencing. Moreover, increased <jats:styled-content style=\"fixed-case\">TSCC CDDP<\/jats:styled-content> cells were arrested in the G0\/G1 phase after knockdown of <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4. Compared with negative control group, the expression of cyclin D1 and p\u2010<jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content> decreased, while that of p\u2010caspase\u20103 and Bax significantly increased.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Silencing <jats:styled-content style=\"fixed-case\">CXCR<\/jats:styled-content>4 may evidently inhibit proliferation, induce apoptosis and enhance cisplatin sensitivity of <jats:styled-content style=\"fixed-case\">TSCC CDDP<\/jats:styled-content> cells by reduced cyclin D1 and p\u2010<jats:styled-content style=\"fixed-case\">AKT<\/jats:styled-content>, and increased p\u2010caspase\u20103 and Bax.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12813","type":"journal-article","created":{"date-parts":[[2018,12,16]],"date-time":"2018-12-16T10:58:51Z","timestamp":1544957931000},"page":"122-128","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["<scp>CXCR<\/scp>4 enhances cisplatin resistance of human tongue squamous cell carcinoma"],"prefix":"10.1111","volume":"48","author":[{"given":"Xiu\u2010Mei","family":"Zhuang","sequence":"first","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Sun Yat\u2010Sen Memorial Hospital Sun Yat\u2010Sen University Guangzhou China"},{"name":"Department of Stomatology Sun Yat\u2010sen Memorial Hospital Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4022-8857","authenticated-orcid":false,"given":"Bin","family":"Zhou","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Malignant Tumor Epigenetics and Gene Regulation Sun Yat\u2010Sen Memorial Hospital Sun Yat\u2010Sen University Guangzhou China"},{"name":"Department of Stomatology Sun Yat\u2010sen Memorial Hospital Sun Yat\u2010sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,12,30]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2011.263"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2013.770"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2013.01.053"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2012.06.010"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/jop.12736"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.oraloncology.2015.08.017"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2017.363"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-15-2888"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.2012.105"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.2741"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.5483\/BMBRep.2014.47.1.069"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gene.2016.08.043"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.37"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccell.2015.05.003"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1220580110"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1186\/1741-7015-10-85"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-12-1092"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-07-4717"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-3803-6"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.3892\/ol.2016.4838"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2013.448"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.03.001"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms3544"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2014.07.025"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2015.01.014"}],"container-title":["Journal of Oral Pathology &amp; 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