{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T16:06:13Z","timestamp":1792166773639,"version":"4.4.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2019,2,19]],"date-time":"2019-02-19T00:00:00Z","timestamp":1550534400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81001206"],"award-info":[{"award-number":["81001206"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:sec><jats:title>Background<\/jats:title><jats:p>Yes\u2010associated protein (<jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content>) is a candidate oncogene in various cancers including oral squamous cell carcinoma (<jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content>). Our previous study demonstrated that <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010alpha could inhibit cell proliferation and invasion by <jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content> phosphorylation in <jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content>. However, the role of <jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content> is not yet clear. The objective of the present study was to elucidate the function of <jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content> in promoting migration in <jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content> and to explore the possible mechanism with a novel <jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content> inhibitor <jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>3.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 68 <jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content> patients were enrolled, and the expression levels of <jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content> were investigated in tissue specimens by immunohistochemical staining. The inhibitory effects of <jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>3, a novel inhibitor of <jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content>, were demonstrated by immunofluorescence, Western blotting, and transwell assays. A human <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> motility array was performed to screen the changes in the gene expression profiles of the cells. In addition, sh<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> interference, <jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content> re\u2010expression, and <jats:styled-content style=\"fixed-case\">WAVE<\/jats:styled-content>1 overexpression plasmids were used to detect the regulatory mechanism of <jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content> and its relationship with cell migration.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Yes\u2010associated protein nuclear expression levels were associated with metastasis and 5\u2010year overall survival rate. <jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>3 exhibited potent inhibitory effects on <jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content> migration. <jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content> knockdown significantly suppressed tumor cell migration in <jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content>. These effects were rescued when <jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content> was re\u2010expressed and during <jats:styled-content style=\"fixed-case\">WAVE<\/jats:styled-content>1 overexpression in <jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content>\u2010sh<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> stable cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The present study revealed that <jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content> was associated with cell migration and that this process was regulated by <jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">WAVE<\/jats:styled-content>1. We also demonstrated that <jats:styled-content style=\"fixed-case\">CA<\/jats:styled-content>3 exhibited marked inhibitory effects on <jats:styled-content style=\"fixed-case\">YAP<\/jats:styled-content> expression and that it could be considered a potential therapeutic target for the treatment of <jats:styled-content style=\"fixed-case\">OSCC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12833","type":"journal-article","created":{"date-parts":[[2019,1,30]],"date-time":"2019-01-30T04:25:13Z","timestamp":1548822313000},"page":"290-298","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Yes\u2010associated protein promotes cell migration via activating Wiskott\u2010Aldrich syndrome protein family member 1 in oral squamous cell carcinoma"],"prefix":"10.1111","volume":"48","author":[{"given":"Lei","family":"Qi","sequence":"first","affiliation":[{"name":"Department of Oral and Cranio\u2010maxillofacial 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