{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T20:06:41Z","timestamp":1789589201107,"version":"build-2803163510"},"reference-count":13,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2019,8,4]],"date-time":"2019-08-04T00:00:00Z","timestamp":1564876800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004901","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de Minas Gerais","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004901","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Oral Pathology Medicine"],"published-print":{"date-parts":[[2019,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Pyogenic granuloma (PG) is a benign nodular lesion with a prominent vascular component which may affect different sites. Recently, <jats:italic>BRAF<\/jats:italic>,<jats:italic> KRAS<\/jats:italic>,<jats:italic> HRAS<\/jats:italic>,<jats:italic> NRAS, GNA11,<\/jats:italic> and <jats:italic>GNA14<\/jats:italic> mutations were reported on PGs of the skin. The present study assessed the role of the MAPK\/ERK pathway in oral PG pathogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mutations in hotspot regions of genes involved in the MAPK\/ERK pathway activation were investigated by Sanger sequencing. The expression of phospho\u2010ERK1\/2 was evaluated by immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Oral PGs did not show mutations in the sequenced regions of the genes <jats:italic>BRAF<\/jats:italic>,<jats:italic> KRAS<\/jats:italic>,<jats:italic> HRAS<\/jats:italic>,<jats:italic> NRAS, GNA11,<\/jats:italic> or <jats:italic>GNA14<\/jats:italic>. Our results also showed activation of the MAPK\/ERK pathway demonstrated by phospho\u2010ERK1\/2 immunohistochemical positivity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Although oral PG shows MAPK\/ERK pathway activation, the driver molecular event remains to be elucidated.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jop.12922","type":"journal-article","created":{"date-parts":[[2019,7,16]],"date-time":"2019-07-16T17:57:35Z","timestamp":1563299855000},"page":"906-910","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Oral pyogenic granulomas show MAPK\/ERK signaling pathway activation, which occurs independently of <i>BRAF<\/i>,<i> KRAS<\/i>,<i> HRAS<\/i>,<i> NRAS, GNA11,<\/i> and <i>GNA14<\/i> mutations"],"prefix":"10.1111","volume":"48","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1632-1533","authenticated-orcid":false,"given":"Tha\u00eds dos Santos Fontes","family":"Pereira","sequence":"first","affiliation":[{"name":"Department of Oral Surgery and Pathology, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Larissa Stefhanne Damasceno","family":"de Amorim","sequence":"additional","affiliation":[{"name":"Department of Oral Surgery and Pathology, School of Dentistry Universidade Federal de Minas Gerais Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6714-3124","authenticated-orcid":false,"given":"N\u00fabia Braga","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Pathology, Biological Sciences Institute Universidade Federal de Minas Gerais Belo Horizonte Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00e9ssica Gardone","family":"Vit\u00f3rio","sequence":"additional","affiliation":[{"name":"Department of Oral 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